TriLink Awarded a Phase II Grant for a Second Hot Start PCR Project

...se I studies demonstrated that TriLink's unique dNTP modifications enhanced PCR performance by increasing amplicon formation while reducing off-target amplicon formation. In the second phase of the program, TriLink will look to further expand upon Phase I studies to develop novel solutions for endpoint PCR, r...

Amplicon in Medical Products

Dynal RELI™ SSO

Description:... PCR amplification with biotinylated primers that flank the specific target region of an HLA locus. This is followed by hybridisation of the resulting amplicon to an array of Sequence Specific Oligonucleotide probes immobilised on a nylon membrane. The immobilisation of these probes is referred to as a Revers...
Company:Dynal Biotech, LLC

SmartCycler 8000 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) by u...
Company:Cepheid

SmartCycler® 8000 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) ...
Company:Cepheid

SmartCycler 9600 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) ...
Company:Cepheid

SmartCycler 6400 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) by u...
Company:Cepheid

SmartCycler 3200 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) by u...
Company:Cepheid

SmartCycler® 3200 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) ...
Company:Cepheid

SmartCycler 4800 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) by u...
Company:Cepheid

SmartCycler® 6400 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) ...
Company:Cepheid

SmartCycler 1600 System

Description:...rietary, sealable reaction tubes that are designed to optimize rapid thermal transfer and optical sensitivity, as well as to reduce the possibility of amplicon contamination. Four-channel optics The SmartCycler System enables the detection of several targets within a single sample (multiplex assays) ...
Company:Cepheid

Amplicon in Biological News

New method developed to identify genetic determinants of Alzheimer's disease

... which was performed under partially denaturing conditions as determined by profiling the mixture of a tested sample and a homozygous standard control amplicon at the given ratio. In almost 300 samples detected, the accuracy of the assay reached 100%. Dr. Tian-Ming Gao, Head of the Neurobiology Department...

Amplicon in Biological Technology

Amplification Efficiency of TaqMan Gene Expression Assays

...ne. Design parameters such as %GC content, Tm, and amplicon length were optimized to ensure that all of the Ta...tions, for example: base composition (26% 79% GC), amplicon length (53 bp186 bp), and secondary structural fea...gure 3. Our assay design pipeline ensures that the amplicon sizes are the shortest possible, and avoids design...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...0-0.999). RT-PCR products from the serially-diluted standards and samples were analyzed by gel electrophoresis to confirm the presence of the specific amplicon (Fig. 3B). To show that LCM enriches macrophage-specific transcripts, RNA was extracted either from whole sections (analogous to homogenized tissue) o...

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

...d using the GS Gene Linker UV chamber to destroy contaminating amplicon or carry-over molecules in microcentrifuge tubes prior to amplif...f distilled water. All of the tubes for the amplification of the amplicon DNA contained 20 ng of amplified β-actin product suspended ...

Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye

...g quantity vs. C(t) cycle for the 100bp amplicon is shown in Figure 1b. The regression coefficient ... amplicon, indicating amplification of the amplicon of interest. Anomalies due to contamina... indicates exclusive amplification of the -actin amplicon as seen in Figure 4. Second, gel electr...

Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System

...cle was set approximately 3C below the T m of the amplicon to minimize any signal from non-specifi... the following primers amplified a 491bp amplicon from the first round PCR product: forwa... primer set was used to amplify a 200bp amplicon from the pUC plasmid in both the primary- ...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

...ed to verify the presence of the lectin amplicon (in all soy) and the EPSPS amplicon (GMO soy specific). ...ur samples failed to generate the epsps amplicon due to an inhibition of the PCR by some component ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... The first example for calculating the temperature range is a 192 bp amplicon from the p53 gene that contains a 40 bp GC-clamp located at the ...les are seen (Figure 4). The second example is a 281 bp amplicon from the -thalassemia gene that contains a 40 bp GC-clamp at the...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

...e, (GC clamp), to the 3' or 5' end of an amplicon results in a single melting domain acros...ile achievable with a GC-clamp where the amplicon melts within a single domain. ... melting temperature. The 3' GC-clamped amplicon contained an eight degree variation at ...

SeeSNP Propietary Analysis Advantages

...ank and source settings to identify regions of the amplicon that produce discordant genotypes in the... The results are reported for the entire amplicon (all) or just the exonic regions of the ... in amino acid changes; 2) remapping the amplicon SNP coordinates to the base coordinate o...

Designing a Successful qRT-PCR Experiment

...s that overlay an intron-exon junction to eliminate signal from genomic DNA contamination. For best results, amplicon lengths of 80 to 200 bp are recommended. Primer and Probe Concentrations vs. Target A...

Amplicon in Biological Products

Universal Amplicon Detection Assay - Colorimetric from CHEMICON

Description: The Light Diagnostics Universal Amplicon Detection Assay (ADA) is intended for use in the qualitative detection and quantitation of DNA generated by in vitro nucleic acid amplification. For research use only; not to be used in diagnostic or therapeutic procedures....
Company:CHEMICON

XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON

Description:...ied product (amplicon). A portion of the resultant amplicon is added directly to the capture plate (included i... separate the two DNA strands. The single-stranded amplicon is captured to the plate via hybridization with a ...he light is directly proportional to the amount of amplicon present in the sample. Components: Denatur...
Company:CHEMICON

XpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™ from CHEMICON

Description:...ion product (amplicon). A portion of the resultant amplicon is added directly to the capture plate and denatur...eparating the two DNA strands. The single-stranded amplicon is captured to the plate via hybridization with a ...he color is directly proportional to the amount of amplicon present in the sample. Components: Denatur...
Company:CHEMICON

Director Universal PCR System from Sigma-Aldrich

Description:...ation) can be completed in one day. The typical cloning efficiency using this method is greater than 80%. Features and Benefits: Universal - PCR amplicon can be cloned into any expression vector High Cloning Efficiency - Typically>80% High Expression Efficiency - Typically>66% High Fidelity - Long...
Company:Sigma-Aldrich