Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
...V irradiation, an aliquot from a master solution mix, containing
enzyme, appropriate primers, reaction buffer, and dNTPs was
* PCR is covered by U.S. Patents issued to Cetus Corporation. amplitaq
is a registered trademark of Cetus Corporation. NuSieve is a reg...
Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
...fold with water), 200 nM of molecular
beacon for GAD67 or 100 nM for β-actin, 500 nM of forward and reverse
primers, 1 U of amplitaq
Gold DNA polymerase (Applied Biosystems), 250
M of each dNTP, 4 mM MgCl 2 , 50 mM KCl, and 10 mM Tris-HCl (pH 8.3). The
Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
... The ABI PRISM BigDye terminator v 3.0
cycle sequencing kit with amplitaq
DNA polymerase, FS was purchased from
Applied Biosystems. The Ta... and fluorescence, Nat Biotechnol 17, 804807 (1999)
and TaqMan are trademarks of Roche Molecular Systems, Inc. One
Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
...a 120 base pair
fragment of the Canine Pancreatic Lipase Gene (Mickel et al., 1989). Amplification
was carried out using 2 Units amplitaq
DNA polymerase (Perkin-Elmer) mixed
with an equal volume of TaqStart antibody (Clontech) per reaction in a
total reaction volume...
Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
...re listed in Table 1. SGI was from
Molecular Probes (S-7567). MasterAmp PCR premix F was from Epicentre Technologies
was from Applied Biosystems (4338856). Reaction components
were assembled in white-shell/white-well Hard-Shell microplates (MJ Research
Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A
PCR amplification of the mixture of 16s rRNA hypervariable regions was
performed using the GeneAmp PCR Reagent Kit with amplitaq
Gold DNA polymerase
(Applied Biosystems, Foster City, CA). A 50 ng aliquot of target DNA was
suspended in 100 l of reaction buff...
Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
...0 mM KCl, 1.5 mM MgCl 2 , 0.001% gelantine), 200 M each dNTP, 0.5
M each primer, 0.3 mg/ml bovine serum albumin (BSA), and 2.5 U
DNA polymerase (Perkin-Elmer Corp.), 10 l DNA solution.
The temperature cycle for the PCR was 60 seconds of denaturation at 94
Pfu DNA Polymerase as a Hot Start Alternative
with various hot start techniques or amplitaq
DNA polymerase, which is a heat-activated version of amplitaq
The results from amplifying a 2...cific
antibody or magnesium wax beads), amplitaq
Gold DNA polymerase
or Pfu DNA polymera...
Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
...human genomic DNA, 500 nM molecular beacon A or G, 400 nM of each primer, 0.2 mM dATP, 0.2 mM dCTP, 0.2 mM dGTP, 0.4 mM dUTP, 3.5 mM MgCl 2 , 2.5 U of amplitaq
Gold DNA polymerase (Applied Biosystems), and 1 U of uracil-N-glycosylase (UNG). Reactions were performed in triplicate in PE Applied Biosystems Gen...
GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems
Description:...2 ribouracil residues in RNA and dUTP itself are not substrates for UNG.3
Gold DNA polymerase, and the other components of the PCR reaction mixture r...
AmpliTaq DNA Polymerase, LD with Buffer I from Applied Biosystems
Description:...nits/L; low DNA contamination; 1 tube of 250 units enzyme/tube and Buffer I amplitaq
DNA Polymerase, LD (Low DNA), is the same enzyme as amplitaq
DNA Polymerase but is further purified through a proprietary separation pro...
AmpliTaq DNA Polymerase, 1,000 units with Buffer I from Applied Biosystems
DNA Polymerase is the most thoroughly characterize...d to guarantee reproducible results.
DNA Polymerase is a 94 kDa ultra-pure, gelatin-fre... lot-to-lot.
The thermal activity profile of amplitaq
DNA Polymerase is ideal for PCR applications becau...
AmpliTaq Gold DNA Polymerase from Applied Biosystems
Gold® DNA Polymerase is a chemically modified ...d in the formation of unintended products. Because amplitaq
Gold® DNA Polymerase is a chemical hot start e...e, there is no worry of biological contamination. amplitaq
Gold® DNA enzyme's chemical hot-start capabili...