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AmpliTaq in Biological Technology

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

...V irradiation, an aliquot from a master solution mix, containing amplitaq enzyme, appropriate primers, reaction buffer, and dNTPs was ad... * PCR is covered by U.S. Patents issued to Cetus Corporation. amplitaq is a registered trademark of Cetus Corporation. NuSieve is a reg...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

...fold with water), 200 nM of molecular beacon for GAD67 or 100 nM for β-actin, 500 nM of forward and reverse primers, 1 U of amplitaq Gold DNA polymerase (Applied Biosystems), 250 M of each dNTP, 4 mM MgCl 2 , 50 mM KCl, and 10 mM Tris-HCl (pH 8.3). The thermal ...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

... The ABI PRISM BigDye terminator v 3.0 cycle sequencing kit with amplitaq DNA polymerase, FS was purchased from Applied Biosystems. The Ta... and fluorescence, Nat Biotechnol 17, 804807 (1999) amplitaq and TaqMan are trademarks of Roche Molecular Systems, Inc. One S...

Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix

...a 120 base pair fragment of the Canine Pancreatic Lipase Gene (Mickel et al., 1989). Amplification was carried out using 2 Units amplitaq DNA polymerase (Perkin-Elmer) mixed with an equal volume of TaqStart antibody (Clontech) per reaction in a total reaction volume...

Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System

...re listed in Table 1. SGI was from Molecular Probes (S-7567). MasterAmp PCR premix F was from Epicentre Technologies (MHF-925F). amplitaq was from Applied Biosystems (4338856). Reaction components were assembled in white-shell/white-well Hard-Shell microplates (MJ Research ...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

... system. PCR amplification of the mixture of 16s rRNA hypervariable regions was performed using the GeneAmp PCR Reagent Kit with amplitaq Gold DNA polymerase (Applied Biosystems, Foster City, CA). A 50 ng aliquot of target DNA was suspended in 100 l of reaction buff...

Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

...0 mM KCl, 1.5 mM MgCl 2 , 0.001% gelantine), 200 M each dNTP, 0.5 M each primer, 0.3 mg/ml bovine serum albumin (BSA), and 2.5 U amplitaq DNA polymerase (Perkin-Elmer Corp.), 10 l DNA solution. The temperature cycle for the PCR was 60 seconds of denaturation at 94 C...

Pfu DNA Polymerase as a Hot Start Alternative

...on with various hot start techniques or amplitaq Gold DNA polymerase, which is a heat-activated version of amplitaq enzyme. The results from amplifying a 2...cific antibody or magnesium wax beads), amplitaq Gold DNA polymerase or Pfu DNA polymera...

Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

...human genomic DNA, 500 nM molecular beacon A or G, 400 nM of each primer, 0.2 mM dATP, 0.2 mM dCTP, 0.2 mM dGTP, 0.4 mM dUTP, 3.5 mM MgCl 2 , 2.5 U of amplitaq Gold DNA polymerase (Applied Biosystems), and 1 U of uracil-N-glycosylase (UNG). Reactions were performed in triplicate in PE Applied Biosystems Gen...
AmpliTaq in Biological Products

GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

Description:...2 ribouracil residues in RNA and dUTP itself are not substrates for UNG.3 amplitaq and amplitaq Gold DNA polymerase, and the other components of the PCR reaction mixture r...
Company:Applied Biosystems

AmpliTaq DNA Polymerase, LD with Buffer I from Applied Biosystems

Description:...nits/L; low DNA contamination; 1 tube of 250 units enzyme/tube and Buffer I amplitaq DNA Polymerase, LD (Low DNA), is the same enzyme as amplitaq DNA Polymerase but is further purified through a proprietary separation pro...
Company:Applied Biosystems

AmpliTaq DNA Polymerase, 1,000 units with Buffer I from Applied Biosystems

Description:... amplitaq DNA Polymerase is the most thoroughly characterize...d to guarantee reproducible results. amplitaq DNA Polymerase is a 94 kDa ultra-pure, gelatin-fre... lot-to-lot. The thermal activity profile of amplitaq DNA Polymerase is ideal for PCR applications becau...
Company:Applied Biosystems

AmpliTaq Gold DNA Polymerase from Applied Biosystems

Description:... amplitaq Gold® DNA Polymerase is a chemically modified ...d in the formation of unintended products. Because amplitaq Gold® DNA Polymerase is a chemical hot start e...e, there is no worry of biological contamination. amplitaq Gold® DNA enzyme's chemical hot-start capabili...
Company:Applied Biosystems
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