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Allelic in Medical News

NYU School of Medicine pathology researchers solve another mystery in B lymphocyte development

...ergoes recombination at a time (a process known as allelic exclusion). In "RAG-1 and ATM Coordinate Monoal...combination of individual alleles to help maintain allelic exclusion. In this sense homologous pairing of Ig ...tion, homologous pairing of Ig loci contributes to allelic exclusion by ensuring that only one allele is targ...

Does 1q31.1-32.1 harbor a tumor suppressor gene related to Chinese SCC patients?

...noma by means of microsatellite markers has become an effective way to find allelic deletion regions and then to find candidate tumor suppressor genes. In a pr...the region might harbor the putative tumor suppressor gene(s). However, the allelic deletion region contained so many genes that it was inconvenient to continu...
Allelic in Medical Products

Applied Biosystems 7500 Real-Time PCR System

Description:... quantitative PCR has many applications including the precise measurement of changes in gene expression, pathogen detection, and post-PCR detection in allelic discrimination (SNPs) assays. It is also ideal for plus/minus assays to confirm the presence or absence of a pathogen, transgene, or other specific ta...
Company:Applied Biosystems

DNA Engine Opticon 2 Continuous Fluorescence Detection System

Description:...st channel, and a range of fluorophores in the second channel including TET, HEX, VIC, and TAMRA for a multitude of applications such as RT-qPCR and allelic discrimination. DNA Engine thermal cycler offers precision thermal control and a temperature gradient feature permits simultaneous incubation at 12 di...
Company:Bio-Rad (formerly MJ Research)

ABI Prism 7000

Description:...quantitative PCR applications include gene expression and pathogen detection. Post-PCR detection is also available for non-quantitative assays such as allelic discrimination (SNP detection) and plus/minus assays....
Company:Abbott Diagnostics

ABI PRISM 7000 Sequence Detection System

Description:...quantitative PCR applications include gene expression and pathogen detection. Post-PCR detection is also available for non-quantitative assays such as allelic discrimination (SNP detection) and plus/minus assays....
Company:Applied Biosystems
Allelic in Biological News

December 2008 highlights from Biology of Reproduction

...d effects can plague researchers studying developmental processes in mammalian organisms. However, because such effects are due to differences in many allelic combinations, they are also key indicators of regulatory complexity. In a paper on p. 1038, Park et al. report genetic background effects on the pheno...

Story tips from the US Department of Energy's Oak Ridge National Laboratory, September 2007

...l findings, said Grisel, associate professor of psychology and neuroscience. There is no significant correlation between the drinking patterns and the allelic status of the taste receptor on Chromosome 2, and many strains of mice voluntarily consumed enough alcohol to become dependent. By studying self-admin...

New discovery: Molecular variation in one gene affects the growth of natural populations

... of the Glanville fritillary butterfly (Melitaea cinxia) is affected by the allelic composition of the phosphoglucose isomerase (Pgi) enzyme. The result challenges previous views according to which allelic variation in populations, and possible consequent differences in individua...

Housecat-sized Siberian tiger cubs get collared

...ir association with DNA markers. The success of disease-association studies is dependent upon several characteristics of the DNA markers, including allelic frequency and genomic coverage. In some cases, a particular variant at one locus is perfectly associated with a specific variant at another locus; in...

Studies on human genome variation provide insight into disease

...ir association with DNA markers. The success of disease-association studies is dependent upon several characteristics of the DNA markers, including allelic frequency and genomic coverage. In some cases, a particular variant at one locus is perfectly associated with a specific variant at another locus; in...
Allelic in Biological Technology

The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B

Luis A Ugozzoli, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Introduction Real-Time PCR PCR has been greatly refined since its advent 16 years ago. Several improvements in the technology and assoc...

GenomiPhi amplification of FFPE tissue DNA for array CGH

... spotted with cloned DNA sequences, such as bacterial artificial chromosome (BAC) arrays, it has become possible to obtain whole-genome information on allelic differences between samples from normal vs diseased tissues (2, 3). CGH experiments can be performed with DNA purified from cell culture or generated ...

Oragene is compatible with TaqMan SNP genotyping

...cher at the 3 end. The reaction components for the allelic discrimination reactions were set up ac...mmended by the manufacturer. allelic discrimination plots Figure 1 shows the allelic discrimination plot for the thymidylate synthetase...

Oragene and Whole Genome Amplification with GenomiPhi

... Figure 2 shows a typical PCR result for WGA and unamplified DNA samples. SNP genotyping Figure 3 shows an allelic discrimination plot for the thymidylate synthetase assay. The plot is presented as the signal (average fluorescence between cycl...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

...uencing of common haplotypes. The use of an outgroup reference standard as a heteroduplex generator greatly improves detection of allelic variants, many of which will have homoduplex molecules with similar electrophoretic mobility. For the oyster 16S product, severa...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

... allele who would be poor metabolizers of many clinical drugs.5 Materials and Methods The Human CYP2D6*4 allelic Discrimination Kit (4312555) and the TaqMan 2X Universal PCR Master Mix (4304437) were obtained from Applied Biosystems. The gen...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

...migrated to similar positions and were consequently binned into 5 allelic groups. For the homoduplex A1 allele fragment...st 70 nt (distal to the GC-clamp) also had apparent influence on allelic migration. Prior to any allele dissociation, alleles s...

Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori

...s a marker for a pathogenicity island, and the allelic variants of the iceA gene. The vacA gene is pres... be distinguished into m1 or m2 subtypes2. The allelic variants of the vacA gene that were detected by ... 8 secretion by gastric epithelial cells3. The allelic variants of the iceA gene are distinguished into...

Pwo DNA Polymerase

... for: cloning of PCR products study of allelic polymorphism in individual RNA transcripts (1, 2) characterisation of the allelic stage of single cells (3) or single DNA molecules (4, 5) ...

Mx4000 Multiplex Quantitative PCR System*,PPP

...nd emission detection system. Applications include allelic discrimination, plate reads, melting cu...efore, such as real-time and plate-read allelic discrimination, real-time QPCR, qualitative detect... The software also features the ability to make allelic discrimination calls by endpoint fluorescence va...
Allelic in Biological Products

DNA Engine Opticon 2 Continuous Fluorescence Detection System from Bio-Rad

Description:...st channel, and a range of fluorophores in the second channel including TET, HEX, VIC, and TAMRA for a multitude of applications such as RT-qPCR and allelic discrimination. DNA Engine thermal cycler offers precision thermal control and a temperature gradient feature permits simultaneous incubation at 12 di...
Company:Bio-Rad
Allelic in Biological Definition

Major histocompatibility complex

...r biology of MHC proteins 3 MHC evolution and allelic diversity 4 See also Introduction ...olutionary biologists, due to the high levels of allelic diversity found within many of its genes. Indeed...e to activation of B cells. MHC evolution and allelic diversity MHC gene families are found in essenti...

Meiosis

...ach gamete carries a sole allele for a gene locus. In addition, the orientation of the metaphase I/II equatorial lining-up is random, resulting in new allelic recombinationis. Independent assortment forms the basis of Mendel 's second law . Lastly, chiasmata causes genetic breaks and the establishment of n...
Allelic in Biological Dictionary

Multi-locus probe

...r tandem repeat probe . ... 3. DNA fingerprinting with a single- locus probe . ... 4. Can be multi -copy locus (i.e., southern blotting) where allelic state ... Probe with labeled. OH 7. Results. 1. Multi - locus ... single- locus Probe RFLP, Multi - locus Probe RFLP, and PCR. 26. Id. a...

Monogenic

...ient who ... monogenic adj. Of or relating to monogenesis; monogenetic. Relating to monogenism. Of or regulated by one gene or one of a pair of allelic genes Monogenic Look up monogenic in Wiktionary , the free dictionary. ... Explain the difference beteen monogenic and polygenic inheritan...

Maternal effect

...a considerable portion of ... linked, maternal effect genes can also facilitate the evol ... wimp is a dominant maternal - effect mutation, allelic to mutations in the second ... The wimp maternal effect only interacts with mutations whose defects are ... The original evidence for gradients...

Linkage disequilibrium

...librium = population wide measure of interlocus allelic associations ... The extent and distribution of...pretation of Linkage Disequilibrium ... ... allelic association (also referred to as linkage disequ...oefficient of disequilibrium . Adjust the initial allelic frequencies and the two-locus fitness values to ...

Intragenic

...nkage ... very informative locally, and intragenic patterns of ... Epistasis is the interaction between genes. ... Intragenic complementation, allelic complementation, or interallelic ... Causes of intragenic complementation include: ... Here we examine the role of the intragenic DMR2 by ge...

Heterozygosity

...tic variability. ... The expected heterozygosity following a bottleneck lasting a single generation ... How does this affect heterozygosity and allelic diversity? ... heterozygosity synonyms, heterozygosity antonyms. Information about heterozygosity in the free ... heterozygosity - the stat...

Genetic drift

... Genetic drift or allelic drift is the change in the relative frequency with which a gene variant (allele) occurs in a population due to random sampling and chance: the ...

Gene frequency

...pies of a particular allele divided by the number of copies of all alleles at the genetic place (locus) in a population. It... Genetic drift or allelic drift is the change in the relative frequency with which a gene variant (allele) occurs in a population due to random sampling and chance: the ...

Gene conversion

...f the gene or if the transferred material ... Two articles regarding theories of rapid evolution through biased gene conversion and loss of allelic variation, leading to the winnowing of the bushy hominid tree. These genes undergo a process of gene conversion in which an internal segment...

Expressivity

...a genetic disease. For example, ... Therefore, when the P allele is present it expresses variable expressivity . Expressivity - variation in allelic expression when the allele is penetrant. ... that make up music performance, the ones to do with expressivity are often regarded as the most ...
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