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Acetyltransferase in Medical News

New chemical can kill latent tuberculosis bacteria

... may have also been passed on to others. In their experiments, the Weill Cornell researchers focused on a bacterial enzyme called dihydrolipoamide acetyltransferase (DlaT). "DlaT's main job is to help M. tuberculosis get energy from nutrients. But when the bacterium is under stress, it also uses the enzyme to defe...

The structure of resistance

... this defense mechanism. Frdric Dardel and colleagues crystallized both the narrow and broad-spectrum resistance forms of the antibiotic-modifying acetyltransferase enzyme. Their report reveals that the enzyme has a flexible active site that can evolve to accommodate new antibiotics, allowing the bacteria to break...

JCI table of contents: Feb. 21, 2008

...tion, and GATA4 acetylation and DNA-binding activity through blocking p300-HAT activity. TITLE: The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats AUTHOR CONTACT: Koji Hasegawa Kyoto Medical Center, National Hospital Organization, Kyoto, Japan. Ph...

Scientists solve structure of gene regulator that plays key role in cancer

...ty to involve other transcription factors in regulating gene expression. About 10 years ago, when scientists discovered that p300/CBP also had histone acetyltransferase activity, Marmorstein and his group began work to determine its three-dimensional structure. But solving that structure was no easy feat. The rese...

Cancer stem cell marker also drives transcription in normal cells

...tion factors, or indirectly, by influencing chromatin. For example, histone acetyltransferase (HAT) complexes are thought to activate gene expression by modifying chroma...ne-tuning of transcriptional regulation by revealing that the hSAGA histone acetyltransferase complex is also capable of histone deubiquitylation. The findings provide c...
Acetyltransferase in Biological News

Workman Lab characterizes novel regulator of chromosome function

... Workman Lab has shed new light on a novel histone acetyltransferase protein complex called ATAC. Acetyltransferases ar...on of the journal. ATAC is unique as the only acetyltransferase protein complex that contains two distinct acetyltransferase enzymes; one that generally activates processes li...
Acetyltransferase in Biological Technology

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

...rs to drive high levels of mRNA and protein expression, vectors containing either a firefly luciferase cDNA or a chloramphenicol acetyltransferase (CAT) cDNA were prepared and transfected into HeLa cells. The close correlation between luciferase or CAT gene transcript levels...

Transfection Reagent Selector Kit Handbook

... Chloramphenicol acetyltransferase ...orters Chloramphenicol acetyltransferase The prokaryotic enzyme chloramphenicol acetyltransferase (CAT) is commonly used as a reporter. Thi...

TransMessenger Transfection Reagent Handbook

...imal expression levels may be obtained earlier following transfection with RNA. Cells transfected with a chloramphenicol acetyltransferase (CAT) reporter RNA are typically incubated for approximately 24 h post-transfection to obtain maximal levels of protein ...

Genetic Reporter Systems

...ptimize electroporation parameters. Chloramphenicol acetyltransferase (CAT) Chloramphenicol acetyltransferase (CAT) This enzyme comes from microorganisms and catalyzes the tr...

Genetic Reporter Systems

...ptimize electroporation parameters. Chloramphenicol acetyltransferase (CAT) Chloramphenicol acetyltransferase (CAT) This enzyme comes from microorganisms and catalyzes the tr...
Acetyltransferase in Biological Products

Chloramphenicol Acetyltransferase Fluorometric (BODIPY) Assay Kit, FAST CAT from Molecular Probes (Invitrogen)

Description: See product name for description....
Company:Molecular Probes (Invitrogen)

Chloramphenicol Acetyltransferase Fluorometric (TMR) Assay Kit, FAST CAT from Molecular Probes (Invitrogen)

Description: See product name for description....
Company:Molecular Probes (Invitrogen)

Mouse Anti-Chloramphenicol Acetyltransferase Monoclonal Antibody, Unconjugated, Clone CAT-1 from Abcam

Description: Mouse monoclonal [CAT-1] to Chloramphenicol Acetyltransferase ( Abpromise for all tested applications)....
Company:Abcam

Mouse Anti-Chloramphenicol Acetyltransferase Monoclonal Antibody, Unconjugated from Novus Biologicals

Description: Mouse monoclonal [CAT-2] to Chloramphenicol Acetyltransferase...
Company:Novus Biologicals

Luciferase Reporter Assay Kit from MBL International

Description:...orter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescenc...
Company:MBL International

Endoplasmic Reticulum Isolation Kit from Sigma-Aldrich

Description:...culture. The isolated ER are useful for protein synthesis, degradation and metabolic pathway analysis. Metabolic abnornalities of ER include carnitine acetyltransferase deficiency in children, cystic fibrosis and many more ER protein retention discorders. Suitable for up to 50g of tissue. Features and Benefits: Spe...
Company:Sigma-Aldrich
Acetyltransferase in Biological Definition

Coenzyme A

...ermore, it contributes the acetyl group to acetylcholine ; the addition of the acetyl group to choline a reaction that is catalysed by choline acetyltransferase . Its main task is conveying the carbon atoms within the acetyl group to the citric acid cycle to be oxidized for energy production. The c...
Acetyltransferase in Biological Dictionary

Intergenic region

...ce ... variation of H3K9Ac enrichment in intergenic regions is the most prominent in ... ... intergenic region fused to a chloramphenicol acetyltransferase (CAT) reporter ... However, the common 740 bp intergenic region showed promoter activity in DDX13 ... ... a way in which intergenic region...

CAT assay

...rovides two methods for monitoring chloramphenicol acetyltransferase activity. ... two alternative methods in the CAT ... (Cm) 10 ┬ÁM ... CAT Assay - Chloramphenicol acetyltransferase ... To perform the enzyme assay , you must firs...aker Si250, Cat #7000-04) ... chloramphenicol acetyltransferase ( CAT Radiometric Assay Kit. Promega. Inquire ....
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