RNA Amplification Just Got Better: MessageAmp II

... II. A comparison of ArrayScript RT (AS; 200 U), SuperScript II (SSII; 200 U); wild type M-MLV RT (200 U), and wild type amv RT (10 U) in MessageAmp II conditions demonstrates that ArrayScript outperforms other commercial RTs. 90 ng HeLa-S3 total RNA (2...

Recombinant HIV Reverse Transcriptase

... More thermostable than amv RT and M-MLV RT Excellen...everse transcriptase (RT) and amv RT, Ambion's HIV RT synthesizes cDNA from single-s...hermostable than M-MLV RT and amv RT HIV RT performs as wel...

Better RNA Amplification

... aRNA Yield. A comparison of ArrayScript RT (AS; 200 U), Competitor RT (Comp; 200 U); wild type M-MLV RT (200 U), and wild type amv RT (10 U) using MessageAmp II conditions demonstrates that ArrayScript outperforms other commercial RTs. 90 ng HeLa-S3 total RNA...

ArrayScript RT: Maximize Yields of Full-length cDNA

...sageAmp II. A comparison of ArrayScript RT (AS; 200 U), SuperScript II (SSII; 200 U); wild type M-MLV RT (200 U), and wild type amv RT (10 U) in MessageAmp II conditions demonstrates that ArrayScript outperforms other commercial RTs. 90 ng HeLa-S3 total RNA (2...

Protector RNase Inhibitor Enhance the Protection of RNA against Degradation

...tageous when performing reverse transcription reactions at elevated temperatures to overcome secondary structure in RNA (e.g., in combination with amv Reverse Transcriptase to generate full length transcripts). Choose an RNase Inhibitor that wont interfere with your ...

Product overview

...condary structure in RNA (e.g. in combination with amv Reverse Transcriptase to generate ful...xploits the superior thermal stability of amv reverse transcriptase for the cDNA synthesis step to reduce secondary structure. amv also provides better processivity and ful...

Reverse transcriptases suitable for two-step RT-PCRd

... + + + + + amv Reverse Transcriptase First Strand cDNA Synthesis Kit for RT-PCR (AMV) ...

Choice of RT-PCR Enzymes

...H activity In contrast to Tth DNA Polymerase, amv and M-MuLV Reverse Transcriptases have RNase H activity. This ac...NA. M-MuLV Reverse Transcriptase has lower RNase H activity than amv Reverse Transcriptase. Mutated enzymes without RNase H activity ...

Properties of Reverse Transcriptases

...p to 14kb 42C (up to 50C) 50 Mg 2+ - - yes amv Reverse Transcriptase up to 12kb 42C (up to 60C) 40 Mg 2+ ++ - ye...

Suggested applications for reverse transcriptases

... +++ ++ n.d. amv Reverse Transcriptase c ++ + ++ ...in the U.S. c amv Reverse Transcriptase is also included in the Titan One Tube...

AMV in Biological Products

GeneRacer Kit with AMV RT and TOPO TA Cloning kit for Sequencing from Invitrogen

Description: GeneRacer is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5' and 3' cDNA ends. With the GeneRacer Kit you can:...
Company:Invitrogen

Rnase-free Ribonuclease Inhibitor from Chimerx

Description:...ee Ribonuclease Inhibitor that is effective over a broad range of temperatures and pH's. This protein does not inhibit SP6, T7, or T3 RNA polymerase, amv and M-MLV reverse transcriptase or Taq DNA polymerase. For bulk purchases contact lloconne@molbiores.com....
Company:Chimerx

GeneChoice RNA Safe Recombinant Ribonuclease Inhibitor from GeneChoice, Inc.

Description:...oes not interfere with the performance of other enzymes in the reaction. • RNA Safe is most effective between pH 5.5 and 8.0. It does not inhibit amv or MMLV reverse transcriptases; SP6, T3, and T7 RNA polymerases; Taq DNA polymerase; RNase H; RNase T1; or S1 Nuclease. • Efficient inhibition o...
Company:GeneChoice, Inc.

M-MLV Reverse Transcriptase from USB Corp.

Description:... RNA:DNA hybrids(1). Full-length copies of large mRNAs, >10 kb, may be synthesized. M-MLV Reverse Transcriptase has a much lower RNase H activity than amv Reverse Transcriptase resulting in high yields of full length cDNA(2). This makes M-MLV Reverse Transcriptase very useful in cDNA synthesis and RT-PCR...
Company:USB Corp.

Reverse-iT One-Step Kit, ReddyMix from ABgene

Description:...e used at temperatures up to 57°C, avoiding problems caused by secondary structure in the RNA template. It also shows a better dynamic range than amv or MMuLV. This means that increases in template concentration give rise to corresponding increases in product generated over a wider range of starting...
Company:ABgene

ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene

Description:...tented blend of RTases combining the advantages of amv and MMuLV RTases to give unparalleled performance....QRTase Blend has greater dynamic range than either amv or MMuLV. This leads to greater accuracy in QRT-PC...e QRT-PCR kits contain a unique blend of MMuLV and amv RTases in a buffer designed for synergy with subse...
Company:ABgene

AMV in Biological Dictionary

Reverse transcriptase

...ve subdomains, fingers (green), palm (yellow) ... Enzyme Storage Buffer: amv Reverse Transcriptase (AMV-RT) is supplied in 200mM ... units of amv Reverse Transcriptase per microgram of template, an oligo(dT) ... En...

Coat protein

...comprises a minor fraction of the phage coat and is ... The faster-migrating major coat protein that has a molecular weight of about 6, ... amv coat protein -replicase interactions ... The conformations of the major coat protein of a filamentous bacteriophage can ... The alpha carb...