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AGAROSE in Medical News

Scientists Build a Better Petri Dish

...n, say the biomedical engineers at Brown University in Providence, R.I., who invented the new dish. The dish is made from a sugary substance called agarose -- long used in laboratories -- that allows cells to self-assemble naturally and form "microtissues," the scientists explained in an article published...
AGAROSE in Medical Products

HYDRAGEL K20 System Manual Agarose Gel Electrophoresis

Description: Hydragel K20 is Sebia's quality line of manual agarose gel electrophoresis products. Samples are applied utilizing the same patented applicator combs that is used for sample application on the HYDRASYS semi-automated electrophoresis system....
Company:Sebia Electrophoresis

HYDRASYS Semi-Automated Agarose Gel Electrophoresis System

Description: Sebia's semi-automated agarose gel electrophoresis system is designed to automate the many tedious steps involved in classic electrophoresis and immunofixation testing. The HYDRASYS system carries out all phases of electrophoresis from sample application to migration to incubation to staining, des...
Company:Sebia Electrophoresis

E-Gel®96 High-Throughput Agarose Electrophoresis System

Description: Turn routine agarose gel electrophoresis into an automated, high-throughput operation with the E-Gel 96 system. This bufferless, pre-cast system is ideal for analyzing multiple PCR products, plasmid preparations, restriction digests, and more. Fully automated, robot-compatible, and ready to put int...
Company:Invitrogen

Mini EZRun Agarose Gel Horizontal Electrophoresis System

Description: Our patented horizontal electrophoresis unit for agarose gel is made of high quality PMMA. It features an unique double-door design for convenience and an electrode protection device.Combs, gel tray and cables are all included gel size : 6.5x6 (cm), 200 ml comb: 2 (9-well) &...
Company:Madell Technology Corporation

UltraPure™ Agarose 1000

Description: UltraPure™ Agarose 1000 is specifically formulated for high resolution of small DNA RNA and PCR fragments <1000 bp. This standard melting temperature agarose can resolve DNA fragments with as little as 10 bp difference in size. UltraPure™ Agarose 1000 gels exhibit a strong gel structur...
Company:Invitrogen

LB Agarose Plates

Description: 1.0% Tryptone, 0.5% yeast extract, 0.5% Sodium Chloride, 1.5% agarose....
Company:Teknova, Inc.

LB Top Agarose 100ml Bottle

Description: 1.0% Tryptone, 0.5% yeast extract, 0.5% Sodium Chloride, 0.6% agarose....
Company:Teknova, Inc.

UltraClean™ MS-8 Agarose

Description: UltraClean MS-8 Agarose is an agarose for molecular screening that improves resolution of small DNA fragments and PCR products. Its main features are: High resolution of PCR and DNA fragments with low base pairs. Improved clarity of the gel, enhancing visibility. Better handling than competit...
Company:Mo Bio Laboratories, Inc.

UltraClean™ Forensic Agarose

Description: UltraClean Forensic Agarose is a premium agarose used for isolating DNA fragments larger than 1000bp. This agarose produces clear sharp bands, with no smearing and no background. Each lot of UltraClean Forensic Agarose is tested and certified to be free of DNase activity, RNase activity and DNA c...
Company:Mo Bio Laboratories, Inc.

UltraClean™ Agarose

Description: UltraClean Agarose, Molecular Biology Grade, is used for the electrophoretic separation of nucleic acids. It can be used to isolate DNA fragments from 0.2 to 40 kb. Each lot of UltraClean Agarose is tested and certified to be free of DNase and RNase activity....
Company:Mo Bio Laboratories, Inc.

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AGAROSE in Biological News

DNA fingerprinting simplified

... KNOXVILLE, TN agarose gel electrophoresis" Most teenagers wouldnt have a...ntly used tools in biochemistry and biotechnology, agarose gel electrophoresis is a common forensic technique... molecules through a gelatinous substance known as agarose by using an electric field. Andrew, in collabor...
AGAROSE in Biological Technology

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

Introduction The transfer efficiency of large (>100 kb) DNAs from agarose gels to membrane is poor unless the DNAs are nicked prior to transfer. Although this has traditionally been accomplished with HCl depurination, UV irradiation ...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acid purification kits Jeff Braman Scott Basehore Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, which provide reliable isolation of high-purity miniprep plasmid,...

Eniva VIBE(R) Nutraceutical Tested at Linus Pauling Micronutrient Research Institute and Found to Possess DNA Protective Properties

... is widely used to assess DNA damage in cancer research, environmental toxicology and radiation biology. After mutagen exposure, cells are embedded in agarose gel on a microscope slide, lysed, electrophoresed, and then stained with fluorescent DNA binding dye. Damaged DNA migrates during the process, forming...

Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

...horter fragments, which can confound experimental results. Thus, it is important to test the RNA integrity. This has been traditionally performed by agarose gel electrophoresis and staining the RNA either with ethidium bromide or SYBR Green dye. In the past, the ratio between the ribosomal bands (28S:18S) ...

QIAGEN Fast Cycling PCR Kit

... Cycling Dye that enables direct loading of the reaction onto an agarose gel without the need to add a gel-loading buffer (Figure 5). Cor...ilitate estimation of DNA migration distance and optimization of agarose gel run time. Fast...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

... Buffer using positive pressure. Results agarose Gel Analysis 96 DNA samples are isolated in quadruplicate from q...Perfectprep 96 kit. A volume of 10 μL from 24 samples were run on a 1% agarose gel containing ethidium bromide (Figure 2). Figure 2: BAC DNA iso...

Amplification Efficiency of TaqMan Gene Expression Assays

... in the form of PCR product to enable the generation of a broad template dilution range. A total of 5 μL of PCR product was run on a 4% agarose gel (NuSieve / SeaKem ) with size and quantitation standards (25 bp ladder from Invitrogen) to estimate the amount of product generated. The remaini...

SNPs Powerful Tools for Association Studies

...fication with locus-specific primers. The amplification products, usually designed to be 100 bp or longer, are then separated on a polymer gel such as agarose or polyacrylamide. Each nucleotide repeat of different length at a locus is called an allele, and some loci can have more than ten alleles. The inform...

Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

...itag containing the sequence information of two independent cDNA tags is punctuated by the anchoring site.* 3.Cloned inserts are verified by agarose gel and sequenced. SAGE analysis tools then analyze these sequences. Finally, a comparison of the sequence data determines variations in expression pa...

Simple Approaches for Optimization of RT-PCR

...lock. Upon completion of thermal cycler program, transfer tubes to ice. 7. Analysis Analyze sample (typically 1 to 10 μl aliquots) by agarose gel electrophoresis with staining by ethidium bromide. Visualize PCR product in gel with a UV transilluminator or fluorescence imager. ...
AGAROSE in Biological Products

Rabbit Anti-DDDDK Tag Antibody, Agarose Conjugated from MBL International

Description: Anti-DDDDK-Tag (Enterokinase Cleavage Site), Agarose Conjugate Research Focus: tag Storage: 4C Shipping Temperature: 4C...
Company:MBL International

Glutathione Agarose Beads from BD Biosciences Pharmingen

Description: Glutathione Agarose Beads 10 ml...
Company:BD Biosciences Pharmingen

Goat Anti-T7 tag Polyclonal Antibody, Agarose Conjugated from GeneTex

Description: Goat polyclonal to T7 tag (Agarose) Immunogen: Goats were immunized with MASMTGGQQMG (T7) conjugated to KLH.After multiple immunizations in Freund's adjuvant, serum was collected....
Company:GeneTex

Goat Anti-T7 Polyclonal Antibody, Agarose Conjugated from GeneTex

Description: Polyclonal Anti-T7 [Agarose Immobilized] Goat Immunogen: Goats were immunized with T7 cleavage site (MASMTGGQQMG). Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Goat anti- human T7 affinity purified antibodies were coupled to agarose beads usi...
Company:GeneTex

UltraClean Agarose from Mo Bio Laboratories, Inc.

Description: Molecular Biology grade. Ideally suited for DNA and RNA separation and recovery. High gel strength. Gelling temp. 36C. Melting temp. 88C. EEO: 0.05-0.13 Resolution 0.2-40 kb; Rnase/Dnase: none detected....
Company:Mo Bio Laboratories, Inc.

UltraClean Low Melt Sieve Agarose from Mo Bio Laboratories, Inc.

Description: Ideally suited for resolving DNA and RNA 10-1000 bases especially PCR products ranging from 100-1000 bp. Gelling temp. (4%) 27C Melting temperature 65C. EEO: 0.10 Rnase/Dnase: none detected...
Company:Mo Bio Laboratories, Inc.

UltraClean Low Melt Agarose from Mo Bio Laboratories, Inc.

Description: Low melting point. Ideally suited for in gel enzymatic reactions. Gelling temp. 24-28C Melting temp. 65C. EEO 0.12 Resolution 0.5-20 kb; Rnase/Dnase: none detected....
Company:Mo Bio Laboratories, Inc.

UltraPure Agarose from Invitrogen

Description: ...
Company:Invitrogen

BacPlaque Agarose from Novagen

Description: Agarose overlays are commonly used in baculovirus plaque assays. Many commercial "DNA grade" agaroses either contain contaminants that are toxic to insect cells or lack the physical characteristics required to obtain good overlays. Novagen's BacPlaque Agarose is pre-qualified in plaque assays and t...
Company:Novagen

AgarPlaque Plus Agarose from BD Biosciences Pharmingen

Description: AgarPlaque Plus Agarose 50 grams...
Company:BD Biosciences Pharmingen

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AGAROSE in Biological Definition

Affinity chromatography

...usion-protein, and possibly antibodies against the GST tag as well. The protein can then be covalently coupled to a solid medium such as sepharose or agarose (these are commercially available). For thoroughness the GST protein and the GST-fusion protein can each be coupled separately. The serum is initially...

Gel electrophoresis

...ids (greater than a few hundred bases ), the preferred matrix is purified agarose . In both cases, the gel forms a solid but porous matrix that looks and fe...istry : Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis . See the " Chain termination method " page for an exam...

Gel electrophoresis

...ids (greater than a few hundred bases ), the preferred matrix is purified agarose . In both cases, the gel forms a solid but porous matrix that looks and fe...istry : Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis . See the " Chain termination method " page for an exam...

Genetic fingerprinting

...o fragments which are separated into bands during agarose gel electrophoresis . Next, the bands of DNA are ...ia a technique called Southern blotting from the agarose gel to a nylon membrane. This is treated with a ra...ical genetic samples with some imperfection in the agarose gel. However, in this case, the laboratory worker...

Molecular biology

...at DNA, RNA, and proteins can all be separated using an electric field. In agarose gel electrophoresis , DNA and RNA can be separated based on size by running the DNA through an agarose gel. Proteins can be separated based on size using an SDS-PAGE gel. Protein...

Polymerase chain reaction

...Figure 3 : PCR product compared with DNA ladder in agarose gel. DNA ladder (lane 1), the PCR product in low c...e PCR product can be identified by its size using agarose gel electrophoresis . agarose gel electrophoresis is a procedure that consists o...

Restriction fragment length polymorphism

...hich only cut where there are specific DNA sequences recognized by the enzymes. The restriction fragments are then separated according to length by agarose gel electrophoresis . The resulting gel may be enhanced by Southern blotting . Result The distance between the locations cut by restriction en...
AGAROSE in Biological Dictionary

Streptavidin

...finity for biotin and is used ... structure of a streptavidin monomer is composed of ... Streptavidin biotin-binding protein immobilized to agarose beads, UltraLink ... Streptavidin generally has less nonspecific binding than avidin (from chicken ... Streptavidin products that produce hig...

Southern blotting

...ern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size-separated DNA to a filter... The Southern blot is used fo...

Southern blot

...thern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size-separated DNA to a filter... Professor Sir Edwin Mellor Southe...

RFLP

...al with sickle cell anemia. ... By performing RFLP analysis we obtain a diversity estimate for a particular ... Prepare a 3% NuSieve (Cambrex) agarose gel for RFLP . ... Perform PCR with Beckman D4-labeled (5'-end) forward 18S rDNA primer and ... Custom D4-labeled primers are ordered from Prol...

Recombinant clone

... AW39R ... Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - DAM1570830 ... Anti-Phosphotyrosine, recombinant clone 4G10®, agarose conjugate. 1 mg. 16-105 ... Anti-Phosphotyrosine, recombinant clone 4G10®, biotin conjugate. 100 µg. 16-184 ... Lecture 54: Recombinant DNA...

Polymorphic loci

... Comparison of agarose type (non- polymorphic loci ) ... These primers amplify polymorphic loci , and alleles of different sizes can be observed. ... Many polymor...

PFGE

...class 2 integron was present in all 14 PFGE type B isolates. ... Mount in gel forming stand and carefully pour 150 ml of 0.8% PFGE certified agarose in 0.5x TBE. ... Gel Preparation (for use with the Bio-Rad CHEF/ PFGE unit) ... posted to typing typhoid typhi plasmid pfge mdr kenya africa b...

Native Gel

... Collagen Gel at mySimon. Smart Shopping at mySimon. Since this is a native gel you are more likely to see a smear of RNA and/or ... Native agarose gel electrophoresis may be sufficient to judge the integrity and ... Native Remedies Clearskin-a Gel 25g and a full online catalog of body ...

Ligase

... The T4 DNA Ligase catalyzes the formation of a phosphodiester bond between ... The T4 DNA Ligase binding to DNA may result in a band shift in agarose gels. ... The T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular ... Uhlenbeck, O.C., Gumport, R.I., T4 RNA ligase , The Enzyme...

Immunoprecipitation

.... Immunoprecipitation and Antibody-Antigen precipitation - Success in an ... Catch and Release Reversible Immunoprecipitation system. Protein G agarose Fast Flow ... Methods, protocols, techniques, tutorials and other information for biomedical students and researchers from the Laboratory of Prof. ...
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