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ACN in Biological Technology

Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80

... peptides by HILIC, the effect of acid and acn concentration were examined. It is comm... The effect of acn concentration on peptide retention was also examin...n of all peptides is possible above an 85% initial acn concentration. Furthermore, recovery of...

Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum

...250 mm, 5 μm Flow rate: 300 μl/min Mobile phase: A-acetonitrile, B-2 mM ammonium acetate Gradient: 5-60% acn in 10 min and hold for 2 min Injection: 10 μl Autosampler: PE series 200 MS Conditions API 2000TM LC/MS/MS Sy...

Fast Multi-Residue Pesticide Analysis in Soil and Vegetable Samples

... 5 m, (250 x 2.1 mm). Elution is performed in gradient mode (Solvent A: water + 10 mM ammoniumformiate + 0.1% formic acid; Solvent B: acn + 0.1% formic acid). Liquid flow rate is 200 l.min.-1 Injection volume is 10 l. Mass spectrometry is performed on an API...

Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry

...olumn (Thermo Hypersil-Keystone) at a flow rate of 700 nL/min with a linear gradient of 0.7%/min from 3% Solvent B (100% acn with 0.1% aqueous formic acid) to 45% B followed by a linear gradient of 1%/min to 80% B. Solvent A was 0.1% aqueous formic acid. ...
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