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An Epitope Tagging Vector for Gene Expression in Mammalian Cells

Stratagene The pCMV-Tag1 vector is an epitope tagging vector designed for geneexpression in mammalian cells. A target gene inserted into the pCMV-Tag1 vectorca...

Epitope-Tagging Vectors for Functional Analysis in Yeast

Stratagene Stratagene has developed a series of epitope-tagging vectors to expressand functionally analyze eukaryotic genes in the budding yeast Saccharomyce...

Oil-Free PCR Using the Hot Top Assembly

96 temperaturecyclers Michelle Cayouette Jane Moores Michael Steege Jason Capodanno Michael Rahilly Stratagene Cloning Systems, Inc. 96 temperature cyclers, researchers can begin...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

Epitope Tagging in E. coli Katherine Felts Denise Wyborski John Bauer PeterVaillancourt Stratagene LIC cloning kit...

Intracytoplasmic Spermatocyte Injection (ICSI)

The number of infertile couples has increased in recent years and is now estimated at 1520% of all couples worldwide. Since its introduction into reproductive medicine in 1978 , in vitro fertilization has been considered a successful method of treating tubal ste...

Chemical Stability of the Brinkmann Bottletop Dispenser and the Brinkmann Bottletop Buret

Bottletop Dispenser and the Brinkmann Bottletop Buret Bottletop dispensers are used in the lab for dispensing a wide range of different solvents from glass or stainless steel containers. These dispensers have to meet various requirements. For example, they must not give...

HotMasterAn innovative Hot Start/Cold Stop technology for better PCR* results

George Halley, Eppendorf 5 Prime, Inc., Boulder, CO, USA Vincent Prezioso, Brinkmann Instruments, BioSytems Application Lab, Westbury, NY Hot Start DNA polymerases have become very popular because of their conven...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently count...

E-business consortium evaluates 12 desktop search engines

Can't find your files? Desktop search applications bring some of the same technology used by Internet search engines to your hard drive, but the market is getting more crowded. The University of Wisconsin-Madison evaluated a dozen desktop search tools for usability, accuracy, versatility, efficiency, security and enterprise readine...

Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

P by Cerenkov counting, a well-known measurement technique that has the...

P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

Introduction This Application Report is part of a series documentingculture growth in the BioFlo 110. With appropriatevessels and control modules, the BioFlo 110 can efficientlygrow yeast and bacteria, as well as mammalian,plant cells and insect cells. Pichia pastoris:...

CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

This Application Report is one in a series documentingsimple protocols for culturing cells in a BioFlo 110 benchtop fermentor/bioreactor system. Themodular BioFlo 110 is offered with an extensivechoice of vessels, impellers and control modules toenable growth of a wide variety of c...

E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor

Introduction This Application Report is part of a series documenting culture growth in the BioFlo 110. With appropriatevessels and control modules, the BioFlo 110 can be used to efficiently grow mammalian cells, plant cells,insect cells, yeast, and bacteria. For this report, a standard 7.5L Bio...

Insect Cell Culture Using a BioFlo 110 Benchtop Bioreactor

Introduction This Application Report is one in a series documentingsimple protocols for culturing cells in a BioFlo 110benchtop fermentor/bioreactor system. The modularBioFlo 110 is offered with a variety of vessels, impellersand control modules to enable growth of a wide varietyof cultures,...

Hybridoma Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

This Application Report is one in a series documentingsimple protocols for culturing cells in a BioFlo 110benchtop fermentor/bioreactor system. The modularBioFlo 110 is offered with an extensive choice of vessels,impellers and control modules to enable growth ofa wide variety of c...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

Over the last several decades, geneticists have learned how to manipulate DNA to identify,excise, move and place genes into a variety of organisms that are quite different genetically fromthe source organism. A major use for many of these recombinant organisms is to produceprot...

Fundamentals of Fermentation: Techniques For Benchtop Fermentors

Part I - E. col Prepared by the R & D Lab, New Brunswick Scientific Co., Inc. The following document outlines step-by-stepprocedures for carrying out a benchtop fermentation,particularly when using an NBS fermentor. Provided in aquestion and answer format, this article cov...

Visions: Broderick says Wisconsin should be in top third in venture capital

re...

Web-based reporting credited with state's top healthcare ranking

Web-based reporting credited with state's top healthcare ranking The AHRQ's data sho...

Barriers will not stop convergence of medical technologies

During residency, which is not the free-for-all carnival that Gray's Anatomy would have us believe, one of the most important judgment calls that is learned is whether the patien...

Midwest offers non-stop flight to North Carolina research park

The ai...

WMEP wins top economic development award

The award, presented at th...

It takes more than money to reach the top

Major search engines continue to update their pay-per click (PPC) infrastructures to increase the...

NIH '05 funding: Midwest has two states in Top 10

(NIH) annual list of spending in grant money by states. The NIH's annual budget, some $28 billion, not only funds research activities in its own institutions in the Washington, D.C./Maryland area, such as the <a href="http://www.cancer...

UW among top biotech patent producers

The study, which included nearly 500 universities worldwide and more than 200 in the United States, was authored by the <a...

Florida schools employ Johnson Controls technology to stop sex predators

Johnson Controls, known more for its attempts to develop hybrid car batteries and energy-efficient building systems, said the security sys...

The top 10 misconceptions of life-science patents

The following is a list of some of the most common and most important misconceptions, surrounded by reality. Like most legal analysis, there are exceptions to any rule or guideline. I'm ho...

Are top performers born or made?

by "Freakanomics" authors Stephen J. Dubner and Steven D. Levitt discuss...

NameProtect releases annual list of top trademarkers

The report provides the busin...

Cisco names Berbee a top partner of 2005

The award recognizes revenue growth and customer satisfaction related to Berbee's sales and support of Cisco telephony products. Pat Scheckel, vice president of Berbee's Cisco practice, said it's the f...

UW-Madison has 7 Sloan research fellows, top in nation

The University of Wisconsin-Madison leads the nation in the number of Sloan Foundation Fellowships in Science and Technology awarded in 2006. UW-Madison's total this year is up to seven, from the two Sloans awarded in 2005. The foundation bestows the awards on young researchers in the fields of chemistry, computer science, economics, mathematics, molecular biology, neuroscience and physi...

Biotech leads list of top U.S. companies to work for

But there are other ways to look at and value the impact of our industry....

Windows exploit worsens as stop-gap measures fill in for official patch

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Computerworld top 100 includes three Wisconsin CIOs

Three Wisconsin chief information officers made the Computerworld magazine's top-100 list this year. They were: Carlton Harper, CIO of , in Racine. Modine specializes in thermal management systems such as cooling for a new soccer stadium in London and a carbon-dioxide-cooled Jeep, and has been active in fuel-cell development.<b...

Madison top-ranked In Digital Cities Survey

The city's Web portal, <a href="...

Top Retailers Choose RedPrairie

, a supply chain technology solutions provider that enables business process transformation, announced today that 55 percent of the top 20 retailers use RedPrairie DLx Labor Workforce Performance Management to optimize their supply chain performance. The retailers including Sears, TJX and Wal-Mart....

New guide ranks UW-Madison tops in research

In September, magazine debuted a college guide that emphasizes outcomes of university work such as public servi...

UW researcher named top innovator

, recognized Blackwell as one of its top 35 innovators under 35 years of ag...

TDS takes top spot in region in customer satisfaction rankings

TDS results in the represent a combined score of...

Top 20 big pharmas represent majority of world pharma market

Though the number of drug blockbusters is on the rise as key Big Pharma companies increasingly globalize their product launches with massive sales forces and globalized pharmaceutica...