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Mouse Tail Genomic DNA Isolation Protocol(1)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. ...

Mouse Tail Genomic DNA Isolation Protocol(1)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. ...

ZyGEM Releases Application Note Showing its DNA Extraction Kits Rapidly Produce High Yields of Quality DNA

...h application. In the application note, ZyGEM researchers describe their experience extracting DNA and determining precise genotypes from mouse tail samples. Researchers performed extractions comparing ZyGEM's prep GEM(TM) Tissue kit with two other commercially available kits with similar protoco...

Berkley Life Sciences Introduces LS Prime: A Revolutionary New Primary Liability Policy for the Life Science Industry

...nses; -- Affirmed punitive damages coverage where allowed by law; -- Blanket additional insured options to suit customer preferences; -- Various tail coverage features that are optional, including: -- Extended Coverage - extends coverage period and reporting period -...

Microscopic 'clutch' puts flagellum in neutral

...ine which hypothesis was correct, the scientists decided it best to let the tail wag the dog. They attached the tail end of the flagellum to a glass slide and examined the movement of the enti...

Genetic pathway critical to disease, aging found

...rry genetic information from the nucleus of a cell to the cytoplasm where proteins are made. Star-PAP is responsible for adding a critical biochemical tail onto messenger RNA. The tail, in kite-like fashion, is necessary for the stability of the messenger RNA molecules, can turn them on and off, and thus ...

Tethered to chip, energy supply that drives sperm could power 'nanobot'

...Veterinary Science looked at the early steps in the glycolysis pathway to see if they could move it from the thin fibrous sheath that covers the sperm tail to a solid inorganic substitutea nickel-NTA (nitrilotriacetic acid) chip. First, the researchers replaced the sperm-specific targeting domain of h...

Researchers report major advance in gene therapy technique

...e artery feeds a whole limb, notes Wolff. In a related procedure, they experienced excellent results with high-pressure injection of genes into the tail veins of rodents, a technique that yielded extensive gene expression in the animals livers. We think the genes traveled from the capillaries throug...

Wake up and smell the networking coffee

... the rounds and at least occasionally show up at a networking gathering: your professional connectedness. When I was a corporate HR person from the tail end of the 1970s through the end of the 1990s, I didnt understand networking. I didnt do it. I started an HR council for the American Electronics A...

Bold prediction: Web 2.0 goes away in 2007

...gets, and mash-up machines will enable non-technically astute individuals to assemble their own applications. Signal surpasses noise The long tail has made for a very noisy, often frustrating, and usually redundant information environment. Amid the overwhelming atmosphere, individuals will hone t...

Data centers: Blowing smoke and raising red flags

...locations having just a portion of their overall capabilities. Do they have full connectivity to every data center, or do certain ones only have a tail circuit from a larger pipe? I cannot answer that either way because I did not see what they had in their facility. But based on their guarded PR perso...

What do CIOs want? Fusion2007 speakers weigh in

...takes can occur anywhere along the solution delivery cycle, and the original business purpose can be lost in the shuffle. Most IT projects are the tail end of a larger business process initiative, Davidson explained. If you look at it that way, then that partnership will always exist because there is ...

CIO Leadership Series: Rick Davidson, Manpower

...gage model, the probability is high that a company will develop something unworkable. It really is a partnership, and most IT investments are just the tail of a larger business-process initiative, Davidson noted. If you always look at it that way, then that partnership will always exist because there is a...

Who is minding the Innovation Gap?

... novel drugs and products reaching the market continues to decline. Some call it the sparse pipeline problem, but that is only one manifestation - the tail end - of the innovation gap. A few definitions are helpful. According to the National Institute of Standards and Technology (NIST), which has pub...

Internet-enabled niche markets take center stage

...derstand the power of what Anderson calls the long tail of niche-market products. The one thing that's ...istribution. It's the culture of the Internet. tail gunners The term long tail refers specifically to the segment of a demand cur...

Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography

...peak start definition. High quality instrumentation and columns are the necessary prerequisite for correct interpretation of tailing peaks the peak tail can be either low molecular polymer or instrumental artefact. The determined Mn values can differ by several hunderts % for the possible interpretat...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

...6.2 mm) at 2.0/1.0 μm optical/pixel resolution, showing the entire saggital section with the anterior (head) end on the right and the (posterior) tail end on the left side. Figure 15 is a fluorescence image of a 20 by 30 mm area of the whole mouse section, displaying the thoraco-abdominal region of t...

Direct Counting of Millipore MultiScreen Filtration Plates.

.... For this assay, we also chose the MultiScreen HV plate (Millipore Cat. No. SA2M060E2). The plate was sterilized and treated with 25% Rat tail Collagen, Type 1 (Collaborative Biomedical Products , Bedford, Massachusetts, #40236) to improve cell growth. 3T3 cells (ATCC, Rockville, Maryland) w...

Analysis of Blood Serum on the Liberty Series II ICP-AES with the axially-viewed plasma

...diluted by a factor of 20. The peak to the left of Cu (327.396 nm) is Sc and causes no problems as it is completely resolved. The tail of the OH peak does contribute slightly to the signal of the Cu (327.396 nm) but this has been successfully corrected using polynomial plotted backgro...

Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

...was investigated. Six mice received one pCMV-S vaccination each by gene gun at weeks 0 and 2, and then antisera were obtained by tail bleeds at weeks 4, 6, 9, and 12 after vaccination. ELISA to Show Antibody Response to pCMV-S DNA Vaccination ELISA ...

RNA Amplification for Array Analysis

... utilizes an oligo(dT) primer containing the T7 RNA polymerase promoter for synthesis of first strand cDNA. The poly(A) tail at the end of mRNA sequences serves as the substrate for the binding of these primers. Since mRNA typically ...

Rapidly Synthesize Large Amounts of Capped RNA

...s. The mMESSAGE mMACHINE T7 Ultra Kit includes reagents for both transcription and the addition of a poly(A) tail to capped transcripts. back to top Ordering Information ...

Highly Sensitive microRNA Array Performance

...e. This strategy maximizes signal from each miRNA without jeopardizing hybridization due to labeling. The 3' tail that is added is a mixture of standard and amine-modified nucleotides. Fluorescent dyes or other detectable ...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

... the miRNA fraction is recovered. Step 3 - The miRNA sample is tailed by poly(A) polymerase. The tail includes an amine-modified nucleotide that reacts with activated Cy3 or Cy5 to fluorescently label the miRNA...

Improving RNA Amplification

...s using the Affymetrix protocol. Additionally, arrays with cRNA from 200 ng total RNA have more under-expressed genes (a broader tail on the left of each plot) for all times measured. This range of differences appears to be consistent with that also seen in Affy...

High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

...rs) were treated with either isoproterenol (agonist) or propranolol (antagonist). The enhanced 2AR has a modified C-terminal tail to increase its affinity for beta-arrestin, resulting in fluorescent vesicle formation. Images were acquired on an ImageXpres...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

...olytic enzyme pepsin. The F(ab) regions comprise the arms of the antibody, which are critical for antigen binding. The Fc region comprises the tail of the antibody and plays a role in immune response. Cells carry antigens (lipids, proteins or carbohydrates) which can be ...

Choice of reverse transcription primers

... are three types of primers that may be used for reverse transcription: Oligo(dT)1218, which binds to the endogenous poly(A) + tail at the 3' end of mammalian mRNA. This primer often produces a full-length cDNA. Random hexanucleotides,...

TransMessenger Transfection Reagent Handbook

...iption reaction. A poly-A tail, a stretch of adenylate residues added 3' to the mRNA coding sequence, also stabilizes mRNA. A poly-A tail can be incorporated into an in vitro-transcribed RNA by using a relevant DNA template or added post-transcriptionally using a poly-A polymera...

The use of TaqMaster PCR Enhancer

... source most commonly used for genotyping is mouse tail (1), however, ear, toe, hair, and blood..., genomic DNA isolation procedures from tail slices involve long incubations (one ho...resence of a TGF alpha transgene. Mouse tail DNA (from last 2 mm of tail) was isolated by Prote...

Intracytoplasmic Spermatocyte Injection (ICSI)

...ment of the transfer capillary across the tail, or by pressing the tail of the sperm cell against the bottom of the dish until it stops moving. The sperm cell is aspirated, tail first into the transfer capillary by rotating the knob of the Cell...

The GeneZoo or the GeneJungle?

.... Our algorithm would place splice variants into different clusters; however since we only use the sequence contiguous to the poly-A tail to perform the clustering, splicing is not a significant factor. We could choose to ignore splice variants when clustering (Figure ...

Fractionating DNA Fragments Generated by Differential Display PCR

...ulations to be compared using an oligo(dT) primer to the poly(A) tail of the mRNA. PCR amplification is then performed using an anchored primer to the poly(A) tail and an arbitrary 5 primer. The PCR amplification produces DNA ...

Easily Amplify Genomic DNA with Long-Distance PCR

... blotting, and does not require purified DNA or the use of radioactive materials. Genomic DNA for PCR screening of mice is generally prepared from tail biopsies 2 or blood samples 3 ; however, the routine procedure of clipping the ear or toe to mark the mice 4 provides enough tissue to extract D...
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