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tagged in Biological Technology

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Introduction Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins ...

New Report Just Published: World Endoscopy Systems Market Report

...odenoscopy / Gastroscopy / Upper Endoscopy II-28 Small Bowel Radiograph II-28 Barium Follow Through Technique II-28 Enteroclysis II-28 tagged Red Blood Cells II-28 Angiography II-28 Virtual Endoscopy II-28 Arthroscopy II-29 Neuroendoscopy II-29 Urology II-29 Cystoscop...

Frost & Sullivan Recognizes Cisbio Bioassays for Technology Innovation

...struct can be easily generated where the SNAP or CLIP tag is attached to the N-terminus of the GPCR. Thus, a fusion protein comprised of the SNAP/CLIP tagged GPCR is expressed on the cell surface. "Given that enzyme substrate reactions have the highest order of specificity associated with them, these r...

Pulmo BioTech Inc. Announces Paper to be Presented at Symposium

...and efficacy of its products could allow it to dominate that market. About PulmoBind PulmoBind uses an intravenously delivered radionuclide tagged molecule which specifically bonds to the inner walls of the circulatory system in the lungs, and by the use of an external Gamma Camera allows an imag...

OriGene Technologies, Inc. Establishes High Throughput Monoclonal Antibody Production Outsourcing Center in China

...s tissue qPCR arrays for biomarker discovery and validation. OriGene also provides a broad range of antibody validation products including genome-wide tagged antigen standards and extensive IHC slides derived from our tissue collection. OriGene is committed to its mission to be "Your Gene Company", in suppl...

Easy assembly of electronic biological chips

...method that incorporates a mixture of biologically tagged nanowires onto integrated circuit chips, according...The Penn State researchers inject a mixture of the tagged nanowires and a liquid over the top of the chip. T...then move the electric field and position the next tagged nanowires. In this proof-of-concept experiment, th...

Pulmo BioTech Announces an Increase in its Stake in PulmoScience

...safety and efficacy of its products could allow it to dominate that market. About PulmoBind PulmoBind uses an intravenously delivered radionuclide tagged molecule which specifically bonds to the inner walls of the circulatory system in the lungs, and by the use of an external Gamma Camera allows an imag...

Pulmo BioTech Announces Appointment of Chief Financial Officer

...safety and efficacy of its products could allow it to dominate that market. About PulmoBind PulmoBind uses an intravenously delivered radionuclide tagged molecule which specifically bonds to the inner walls of the circulatory system in the lungs, and by the use of an external Gamma Camera allows an imag...

DNA sensors found to be an effective artificial nose

...hn Kauer and colleagues demonstrate a previously unreported property of deoxyribonucleic acid. The researchers show that single-stranded DNA molecules tagged with a fluorescent reporter and dried onto solid surfaces can respond to vapor phase odor pulses in a sequence-selective manner. In the context of ...

Tethered to chip, energy supply that drives sperm could power 'nanobot'

...kinase, the first enzyme of glycolysis, with a tag that binds to a special gold surface. Even when tethered, the enzyme remained functional. Next they tagged the second enzyme in the pathway, glucose-6-phosphate isomerase. This too was active when tethered. With both attached to the same support, the enzyme...

Acorn Acquisition Corp Announces Definitive Agreement to Acquire Lumen Medical Inc.

...titute) and Dr. Jocelyn Dupuis (the originator of the PulmoBind Molecular Imaging technology). PulmoBind uses an intravenously delivered radionuclide tagged molecule which specifically bonds to the inner walls of the circulatory system in the lungs, and by the use of an external Gamma Camera allows an imag...

Individual differences caused by shuffled chunks of DNA in the human genome

... novel DNA-based methodology called Paired-End Mapping (PEM). Researchers broke up the genome DNA into manageable-sized pieces about 3000 bases long; tagged and rescued the paired ends of the fragments; and then analyzed their sequence with a high-throughput, rapid-sequencing method developed by 454 Life S...

Spend Money to Make Money and Dump Obsolete Tech Strategies

...force that affected the anticipated outcome. No one ever admits that the idea was a bad one in the first place and many times the failed strategy isnt tagged to its originator. There are some outcomes where the initial decisions cannot be overlooked. When Andersen was caught with integrity problems with...

RedPrairie Builds RFID Lab - Enables Real-World Testing

...om a major food manufacturing company used the high-tech RFID Lab environment to experience RFID technology in action. The delegates were able to send tagged cases and pallets through RedPrairie's RFID portal to evaluate the equipment and process. One end user notes, "RedPrairie is ahead of the competition ...

From Rustbelt to Techtown: Milwaukee is Building a New Image and a New Economic Reality.

...ashington, Racine, Kenosha and Walworth counties. These firms employ 21,550 workers with a total annual payroll of about $1.2 billion. The report also tagged 75 biomedical firms, employing 4,000 workers with a total annual payroll of $250 million. In addition, the report inventoried trends relating to th...

Upgrading your business with RFID

...stem upgrades, Accenture has instituted a short-run trial system in which a limited amount of product enough to fill four stores for three months is tagged and kept separate, and then put through standard circulation. This creates a safe and secure chain, as the RFID scanners monitor every step of the pro...

Don't count out notebooks

...n step with the return to being a "pure-play" security company . . . According to a new security warning, pop-up ads are now packing a powerful punch. tagged as an executable, the masked Trojan horse gathers up personal financial information as users head to sites like Citibank and Deutsche Bank. The add-on...

UW-Milwaukee Chancellor Santiago determined to build on research base

...ashington, Racine, Kenosha and Walworth counties. These firms employ 21,550 workers with a total annual payroll of about $1.2 billion. The report also tagged 75 biomedical firms, employing 4,000 workers with a total annual payroll of $250 million. Milwaukee is slowly transforming its image from that of a...

North-woods beef jerky maker adopts RFID to track shipments

...ot under pressure from customer mandates, Veeramani said. Jack Links and ABC will be implementing RFID technology in four stages. They have already tagged some cases and pallets, and are now integrating the technology into Microsofts Navision program. ABC Computers and other Microsoft partner companies a...

Living the sweet life... in IT?

...aking at the annual kick off meeting (or not having to speak depending on your preference) or even Valentines day in Paris with your significant other tagged onto a business trip. What is your personal and professional dolce vita ? What are you doing to make it happen? When you go wondering off into the...

Tommy Thompson to get RFID implant

...shed himself as the first Wisconsin governor elected to four consecutive terms, and he has now set a new precedent: the first Wisconsin governor to be tagged with radio frequency identification for digital access to his medical records. Tommy Thompson Thompson, who also served as Secretary of Health a...

RFID technology is opportunity for Wisconsin companies

...Clark is a pioneer in the adoption and implementation of RFID technology. In May 2004, the former Wisconsin-based consumer-packaged goods manufacturer tagged and shipped cases and pallets of two of its products to Wal-Mart's Sanger, Texas, distribution facility during a successful live test of radio-frequen...

Pacific Cycle rides full speed into RFID

...cerns remain. With the box tagging, a better connection to the actual bike is established for the distribution and retail channels that really use the tagged information. That close link to business drivers has shown up in other ways in the RFID project. Matthews had to sell the idea of this large, innov...

Old bell system merging back together, but it's not the same

...ooks. Asked one former pre-divestiture Illinois Bell account executive: "What innovation is going to come out of the merger?" Some assets that were tagged to be spun off to the RBOC in 1984 will be spun back into the nouveau Bell system all at the expense of the ratepayer. What has the consumer really go...

Lights, cameras, action: The state of online video

...hing and enhancing video search engine capabilities (specialty search engines like Dabble , PureVideo , SearchVideo , and SearchforVideo using tagged content and starting to incorporate voice recognition technologies). How can your business use video? There are various ways that your busines...

AT&T - BellSouth merger: Revestiture continues

...istic on siding with some party and its views on every issue versus understanding that there are some things that are more important and should not be tagged with party affiliation. Concessions should be given to AT&T if they, in return, are going to build and maintain the best infrastructure in the worl...

Georgia healthcare system to install GE's radio frequency ID

...on, reduce the staff time spent locating equipment, and reduce equipment spending, itself. The technology uses a proprietary RFID network to locate tagged equipment within a medical facility. It operates through a separate network that does not interfere with existing wired or wireless networks, and does...

Outflanking the Google, Yahoo, MSN juggernaut

...search, Mamma and Dogpile ; RSS search, Feedster ; and shopping search. Knight encourages visitors using his list to explore the sites that he has tagged with an honorable mention. Multitude of options So what do all these developments mean for you and your business? One clear implication is tha...

Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals

... Combined multiwavelength fluorescence and X-ray imaging is shown in Figure 3 . We injected three different fluorescently tagged imaging agents subcutaneously into different regions of the mouse abdomen. Fluorescence imaging with different filter sets appro...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

... by beam scanning in the X (lateral) direction and stage scanning in the Y (longitudinal) direction of the specimen, to produce images in the standard tagged image file format (TIFF). The main com- ponents of the optical module are the laser scan lens, excitation/illumination optics, and fluorescence and RG...

FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

...ound addition. METHODS Cells HEK-293 cells were used for the transient expression of the 5-HT2C (VSV isoform, tagged at the C-terminus with GFP), 5-HT 1A , 5-HT 6 or 5-HT 7 receptor subtypes. The 5-HT 2C receptor couples primarily via Gq to the activation of phosp...

Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers

... ubiquitin-mediated proteolysis, an ATP-dependent process where unwanted proteins are multiply-tagged with ubiquitin (a 76-amino acid protein). 1 The tagged proteins are then transported to the proteasome for degradation. The proteasome is a massive (2.5 MDa), barrel-shaped protein complex inside all eukar...

Immunoassay of Theophylline by fluorescence polarisation

...Discussion Figure 4 shows excitation and emission spectra of bound, tagged theophylline and antibody taken with a bandpass of 7.2 nm on the excitation... maximum occurs at 485 nm, while the emission maximum occurs at 513 nm. The tagged drug shows virtually no polarization when not bound to the antibody,...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

...ible to use FRET to measure conformational changes in a molecule tagged with two GFP variants in response to the binding of ligands such... as calcium1, or the interaction between separate proteins, each tagged with a specific GFP5. Studies such as these present the unique opportunity ...

Microgenomic Expression Profiling

...mple homogeneity for accurate nucleic acid quantitation when using the NanoDrop ND-1000 spectrophotometer. ◊ Protect fluorescently tagged nucleotide conjugates from long exposures to light. 1. Laser Cut and Laser Capture Microdissection: Isolate specific cel...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

...mulation (4,5).

Using N-terminal VSV-G tagged receptors and CypHer5E-labeled anti-VSV-G antibodi... HEK293 cell line expressing VSV-G epitope tagged β2-adrenergic receptor HEK293 cell line expressing VSV-G epitope tagged δ-opioid receptor HEK293 cel...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

...5 labelled anti-VSV-G antibody to detect the VSV-G tagged receptors.

Epidermal growth factor recep... HEK293 cell line expressing VSV-G epitope tagged β 2-adrenergic receptor

HEK293 cell line expressing VSV-G epitope tagged δ -opioid receptor

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

...ated with a clonal stable HEK 293 cell line expressing N-terminally epitope tagged β2-adrenergic receptor. Upon agonist stimulation, a fluorescent signal... library (Sigma)

HEK293 cell line expressing VSV-G epitope tagged β2-adrenergic receptor

Culture medium: MEM ...

Addition of imidazole during binding improves purity of histidine-tagged proteins

...teins elute with the target protein and must be subsequently removed. In general, the binding affinity of these contaminants is lower than that of the tagged recombinant proteins, allowing their separation from the protein of interest by using more stringent conditions.

There are several ...

Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays

... Bio-Plex assays allow detection of mutiple target proteins in a small volume of sample. The assays are based on detection of signal from tagged fluorescent beads (xMAP technology) that have bound to each target protein. Typically, each type of bead is conjugated with dist...
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