Pilot Study Finds Adding Topical Imiquimod To Laser Therapy Improves The Response Of Port Wine Stain Birthmarks
Two-thirds of all port wine stain
birthmarks occur on the face. As such, there is a strong need for an effective, long-lasting treatment that safely fades these reddish-purple skin lesions that can have a huge impact on a patient's physical and emotional well-being. Now, a new study evaluating ...
Improved Sensitivity for Staining RNA with SYBR Gold Stain
Eagle Eye II still video system provides quality images from
nanogram quantities of RNA
Genina Troutman Ian Miller Jeff Mueller Mike
The SYBR Gold nucleic acid gel stain
(Molecular Probes), combined with Stratagenes Eagle Eye II stil...
Microban Partners With YogaDirect.com to Launch Yoga Mats With Zinc Antimicrobial Technology
On an unprotected mat, microbes can double in number every 20 minutes. Microban antimicrobial protection works continuously to fight the growth of stain
and odor causing bacteria and fungus, keeping YogaDirect's mats cleaner between cleanings. And, because Microban technology is infused into the yoga m...
Hill-Rom Innovative Envision(R) E700 Wound Surface Receives Gold Medical Device Excellence Award
...athable, 3-D fabric with crush resistant channels increasing air circulation and flow beneath the patient. In turn, the high moisture vapor permeable, stain
resistant cover improves the ability to manage heat and moisture directly beneath the patient. The product also offers PressureSmart(TM) Sensing Techn...
DuPont Launches Innovative Renewably Sourced Material for Carpet Cushion
...nce and environmental benefits. Performance benefits include exceptional softness, crush resistance and durability in addition to permanent, built-in stain
resistance that will never wash or wear off. The carpet has a better environmental footprint than traditional nylon 6 carpets with less energy used a...
FDA Approves DORIBAX(TM) for the Treatment of Complicated Intra-Abdominal and Complicated Urinary Tract Infections
...visit http://www.jnj.com .
(1) Gram-negative indicates a group of bacteria that become red when the bacterial cells are treated using the Gram stain
method. This response is based on the chemical and physical properties of their cell walls and is used to identify the type of bacteria. Som...
DuPont, Genencor International and Tate & Lyle Honored for Groundbreaking Work in Developing Bio-PDO(TM)
...t will forever
change the way we design our products and facilities." DuPont discovered a unique polymer in the 1940s that was known for its
resistance, UV resistance and other valued performance
attributes. The polymer, now known as DuPont(TM) Sorona(R) renewably
sourced polymer, required ...
SportCoatings; The North American Booster Club Association (NABCA); the Coatings Specialist Group New Booster Program Goes High Tech With Invisible Antimicrobial Defense
...n old and new fabrics alike, FabricAide remains active
24/7 and is long lasting. It will not change the properties of the fabric
and inhibits odor and stain
causing bacteria from growth, eliminating the
famous "sweat smell." "With FabricAide, parents can obtain priceless peace-of-mind that their
Improving the accuracy and speed of mammalian cell counting
...between sampling and analysis increases. Furthermore, the naked eye can only differentiate pigment intensities in a limited concentration range of the stain
in the hemocytometer chamber. Combining these drawbacks with the potential for cell aggregation, it is easy to see why variation in cell counting is c...
LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
...o fluorescent dyes, calcein AM (cal AM) and ethidium homodimer (EthD-1), to stain
live and dead cells simultaneously. Cal AM is an electrically neutral, non... ~525nm when excited at ~485nm. In contrast, EthD-1 is a polar nucleic acid stain
that can penetrate dead, but not live cell membranes. Once intercalated int...
Quantitative Analysis of the Cell Cycle in Imaging Applications
...ycle phase. In addition, the nature of the exchangeable antibodies allows this assay to be multiplexed with other applications. For example, the Ki-67 stain
could be substituted with a marker for pathway activation. Therefore this assay can work as a system of building blocks where users can choose their p...
Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
...nd 2). Alternatively, HUVEC cells are detected by the addition of CentriRed stain
from Applied Biosystems (Figures 3 and 4). A standard curve, generated by a...
Dye B-labeled anti-ICAM-1, antibody (Figures 1 and 2) or CentriRed stain
(Figures 3 and 4) 5.Incubate overnight at room temperature and scan.
Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
...nation of the histochemical acetylcholine esterase stain
by Karnowsky and Roots 5 and immunohistochemical ... at -80C.
3. For the acetylcholine esterase stain
by Karnowsky and Roots, the following solution is ...te out of solution after 2 hours.
4. To stain
slow MyHC positive fibers rapidly Molecular Probes...
Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
... 3) Wash with DEPC-treated distilled water for 30 seconds.
with Mayers hematoxylin (DAKO, Cat. # S3309) for 30 seconds. 5) ...or 30 seconds.
8) Dehydrate with 95% ethanol for 30 seconds. 9) stain
with Eosin (Sigma, Cat. # E6003) for 30 seconds. 10) Place the slides t...
Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
...u;l in 100-ml PBS and add 100-μl/well. Incubate for 30 min at room temperature.
6. Wash wells three times with PBS.
7. stain cells with 5-μM Hoechst™ 33342 in PBS for 15 min at room temperature.
8. Image wells using excitation and emission settings fo...
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
... was carried out at 60 C for 15 h at 200 V. The gel was subsequently soaked
for 30 min in 1/10,0000x strength SYBR Green I nucleic acid gel stain
(Molecular Probes, Inc., Eugene, OR) and visualized under UV-light.
Results and Discussion
Primer pair ...
Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A
with iodoacetamide containing buffer. Following electrophoresis, gels
were fixed for 30 min, stained with Bio-Safe Coomassie stain
for 1 hr,
and then destained for at least 2 hr before scanning with a GS-800 densitometer.
Protein Identification ...
Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A
Blue for 48 hr and restained for a further 48 hr with fresh stain; one
gel was stained with SYPRO Ruby protein gel stain
(image not shown). The
gels were destained with and stored in water. Gel images were acquired
on an Agfa Arcus II scanner throug...
The Bio-Rad Silver Stain, Rev E
...staining was first introduced as a general protein stain
in polyacrylamide gel analysis i...l. 54 This first practical
PAGE silver stain
was of the silver diammine type adapted from early...ore reliable,
and very sensitive silver stain
derived from a photographic chemical process.
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
...elative quantitation accuracy
is often highest for proteins that stain
with similar efficiency to the
upper marker protein.
B...discussed previously. Thus, a protein with poor
affinity for the stain
would have to be present at concentrations above
2.5 ng/l to be ...
Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C
...isease (Figures 4C, F, I, L, and O) obtained from
a patient with invasive disease. As depicted in the figure, the tumor
does not stain
with keratin 19 antibodies (Figure 4B), is BG3C8-antigen
positive (Figure 4E) (Celis et al. 1996b), keratin 13 negative (Figure
Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A
... volts until
the dye front reached the bottom of the gel. Following electrophoresis,
the gel was silver stained using the Silver stain
Plus kit (Bio-Rad).
DNA POLYMERASE α ASSAY
DNA polymerase α activity was assayed with an activated calf thymus DNA
UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
Note: This protocol must be followed rigorously to obtain optimal results.
1. Following electrophoresis, stain
the gel with 1.0 g/ml ethidium bromide
(EtBr) for exactly 30 minutes with constant agitation. Use a fresh dilution
of EtBr for e...
Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
...00 constant volts
and at 60 C, constant temperature. Individual bands on the parallel DGGE
gels were visualised using SYBR-Green stain
and UV illumination.
Results and Discussion
A scheme for identifying HLA-DRB alleles using DGGE is shown in Figure...
Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B
... 10% polyacrylamide resolving, 3% stacking gel using the Mini-PROTEAN
II slab cell (Figure 2). Gels were stained using the Bio-Rad Silver stain
Chlorella sorokiniana NADP-specific GDH was purified an additiona...
Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
... the genomic DNA was extracted from paraffin
embedded materials. In such cases we used SYBR Green I
instead of ethidium bromide to stain
the gels. This improved
sensitivity and enabled us to see the bands clearly.
These experiments indicate that DGGE is a p...
Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
1A). Fractions 913 were pooled and concentrated. The presumed M-protein
was electrophoresed and detected by Coomassie blue stain
(Figure 1B, lane
5) and its virus specificity was confirmed by western blot (Figure 1C,
lane 5). After concentration of fraction...
Protect RNA and Improve Target Cell Identification
...nuclei, and nucleoli. This histological
has been used to stain
neurological tissues and is especially useful
Acridine Orange stain
intercalates into nucleic acids
Fast, Accurate Assessment of siRNA-induced Gene Silencing
qRT-PCR with SYBR Green Detection
SYBR Green is an economical nucleic
that binds to double-stranded DNA and can be used
to detect PCR products on most real-time detection platforms.
Drive Long-Term Silencing with CMV Vectors
the resulting reduction in protein expression was examined by fluorescence
microscopy. The cells were mounted with DAPI to stain
nuclei (Blue). Images
were digitally captured and quantified using Metamorph software.
The p Silencer 4.1...
Advancing the quality control methodology
to assess isolated total RNA
and generated fragmented cRNA
interpret and the chip runs on the
bioanalyzer are less time consuming.
It can take up to an hour and
half to prepare the samples, run a
the gel, and take a picture.
The whole Agilent process
(sample preparation to saving the
file) can be done in half that time.
There is no impr...
Quality assurance of RNA derived from
laser microdissected tissue samples
obtained by the PALM MicroBeam System
using the RNA 6000 Pico LabChip kit.
with the RNA Pico Assay, but
significant differences in RNA
yield and quality were observed.
Best results were obtained using
the MG stain
approx. 3100 pg; highly intact
RNA; see fig. 3A). HE (figure 3B)
and NFR staining (figure 3C) gave
very similar results in ter...
Comparison of different protein
(Waldbronn, Germany). All SDSPAGE
reagents and gels were purchased
from Invitrogen GmbH
Destain Solution were purchased
from BIO-RAD Laboratories
GmbH (Munich, Germany). The
digital camera and the imaging
Detection of antibody-stained cell
surface and intracellular protein targets
with the Agilent 2100 bioanalyzer
buffer after harvesting.
cells with calcein (up to
5 x 106/ml, 0.5 M calc...
3. Wash once in staining buffer.
cells (1 x 106) in a total
volume of 100 l (80 l...nd centrifuge (500 x g,
cells (1 x 106) with GR
antibody in a total volu...
Flow cytometric analysis of human
primary cells using the Agilent 2100
bioanalyzer and on-chip staining
...he exact adjustment of cell concentration
and density of samples
with few cells. Cells were transfected
and stained on-chip with
the live cell stain
described in reference 2. Only
30,000 cells were required for onchip
staining of primary cells and subsequent measurement on the
Optimizing electroporation parameters
...ons. The easiest way of doing so is by
incubating the cells for 30 minutes in hypoosmolar buffer and then performing
a viability stain
using trypan blue or propidium iodide. If observation
under a microscope reveals lysis in more than 10% of the cells, the osmolarity
High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
...ded satisfactory efficiencies. Twenty-four hours
after transfection, we used Stratagenes in situ b-galactosidase
staining kit to stain
cells with X-gal, and the resulting cells that expressed
for b-galactosidase were photographed ( Figure
Eagle Eye Optical Filter Optimized for Imaging Silver-Stained
1 shows a gel as imaged by the system using either the ethidium bromide
(panel A) or silver stain
(panel B) optical filter with transillumination.
2 shows an image of the gel obtained using the same filters but with
hNT BlueNeurons Express b-Galactosidase at High
hNT BlueNeurons have high levels of beta-galactosidase activity and stain
dark blue in standard assays. This feature is especially useful for transplantation studies of CNS tissues, where the ability to stain
for beta-galactosidase activity allows hNT BlueNeurons to be easily disting...
Optimized Imaging of Protein Gels Stained with Coomassie
Brilliant Blue Dye
...Filter, an optical bandpass filter
designed for SYBR Green nucleic acid stain
CAT applications. 1 The Evergreen Filter maximally overlaps ...ye. Proteins stained with Coomassie blue appear blue in color
because the stain
absorbs non-blue wavelengths of visible light while reflecting