stain in Biological Technology

Pilot Study Finds Adding Topical Imiquimod To Laser Therapy Improves The Response Of Port Wine Stain Birthmarks

Two-thirds of all port wine stain birthmarks occur on the face. As such, there is a strong need for an effective, long-lasting treatment that safely fades these reddish-purple skin lesions that can have a huge impact on a patient's physical and emotional well-being. Now, a new study evaluating ...

Improved Sensitivity for Staining RNA with SYBR Gold Stain

Eagle Eye II still video system provides quality images from nanogram quantities of RNA Genina Troutman Ian Miller Jeff Mueller Mike Kobrin Stratagene The SYBR Gold nucleic acid gel stain (Molecular Probes), combined with Stratagenes Eagle Eye II stil...

Microban Partners With YogaDirect.com to Launch Yoga Mats With Zinc Antimicrobial Technology

... On an unprotected mat, microbes can double in number every 20 minutes. Microban antimicrobial protection works continuously to fight the growth of stain and odor causing bacteria and fungus, keeping YogaDirect's mats cleaner between cleanings. And, because Microban technology is infused into the yoga m...

Hill-Rom Innovative Envision(R) E700 Wound Surface Receives Gold Medical Device Excellence Award

...athable, 3-D fabric with crush resistant channels increasing air circulation and flow beneath the patient. In turn, the high moisture vapor permeable, stain resistant cover improves the ability to manage heat and moisture directly beneath the patient. The product also offers PressureSmart(TM) Sensing Techn...

DuPont Launches Innovative Renewably Sourced Material for Carpet Cushion

...nce and environmental benefits. Performance benefits include exceptional softness, crush resistance and durability in addition to permanent, built-in stain resistance that will never wash or wear off. The carpet has a better environmental footprint than traditional nylon 6 carpets with less energy used a...

FDA Approves DORIBAX(TM) for the Treatment of Complicated Intra-Abdominal and Complicated Urinary Tract Infections

...visit http://www.jnj.com . (1) Gram-negative indicates a group of bacteria that become red when the bacterial cells are treated using the Gram stain method. This response is based on the chemical and physical properties of their cell walls and is used to identify the type of bacteria. Som...

DuPont, Genencor International and Tate & Lyle Honored for Groundbreaking Work in Developing Bio-PDO(TM)

...t will forever change the way we design our products and facilities." DuPont discovered a unique polymer in the 1940s that was known for its inherent stain resistance, UV resistance and other valued performance attributes. The polymer, now known as DuPont(TM) Sorona(R) renewably sourced polymer, required ...

SportCoatings; The North American Booster Club Association (NABCA); the Coatings Specialist Group New Booster Program Goes High Tech With Invisible Antimicrobial Defense

...n old and new fabrics alike, FabricAide remains active 24/7 and is long lasting. It will not change the properties of the fabric and inhibits odor and stain causing bacteria from growth, eliminating the famous "sweat smell." "With FabricAide, parents can obtain priceless peace-of-mind that their student a...

Improving the accuracy and speed of mammalian cell counting

...between sampling and analysis increases. Furthermore, the naked eye can only differentiate pigment intensities in a limited concentration range of the stain in the hemocytometer chamber. Combining these drawbacks with the potential for cell aggregation, it is easy to see why variation in cell counting is c...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

...o fluorescent dyes, calcein AM (cal AM) and ethidium homodimer (EthD-1), to stain live and dead cells simultaneously. Cal AM is an electrically neutral, non... ~525nm when excited at ~485nm. In contrast, EthD-1 is a polar nucleic acid stain that can penetrate dead, but not live cell membranes. Once intercalated int...

Quantitative Analysis of the Cell Cycle in Imaging Applications

...ycle phase. In addition, the nature of the exchangeable antibodies allows this assay to be multiplexed with other applications. For example, the Ki-67 stain could be substituted with a marker for pathway activation. Therefore this assay can work as a system of building blocks where users can choose their p...

Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System

...nd 2). Alternatively, HUVEC cells are detected by the addition of CentriRed stain from Applied Biosystems (Figures 3 and 4). A standard curve, generated by a... Dye B-labeled anti-ICAM-1, antibody (Figures 1 and 2) or CentriRed stain (Figures 3 and 4) 5.Incubate overnight at room temperature and scan. ...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

...nation of the histochemical acetylcholine esterase stain by Karnowsky and Roots 5 and immunohistochemical ... at -80C. 3. For the acetylcholine esterase stain by Karnowsky and Roots, the following solution is ...te out of solution after 2 hours. 4. To stain slow MyHC positive fibers rapidly Molecular Probes...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

... 3) Wash with DEPC-treated distilled water for 30 seconds. 4) stain with Mayers hematoxylin (DAKO, Cat. # S3309) for 30 seconds. 5) ...or 30 seconds. 8) Dehydrate with 95% ethanol for 30 seconds. 9) stain with Eosin (Sigma, Cat. # E6003) for 30 seconds. 10) Place the slides t...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

...u;l in 100-ml PBS and add 100-μl/well. Incubate for 30 min at room temperature.

6. Wash wells three times with PBS.

7. stain cells with 5-μM Hoechst™ 33342 in PBS for 15 min at room temperature.

8. Image wells using excitation and emission settings fo...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... was carried out at 60 C for 15 h at 200 V. The gel was subsequently soaked for 30 min in 1/10,0000x strength SYBR Green I nucleic acid gel stain (Molecular Probes, Inc., Eugene, OR) and visualized under UV-light. Results and Discussion Primer pair ...

Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

...min with iodoacetamide containing buffer. Following electrophoresis, gels were fixed for 30 min, stained with Bio-Safe Coomassie stain for 1 hr, and then destained for at least 2 hr before scanning with a GS-800 densitometer. Protein Identification ...

Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A

...dal Coomassie Blue for 48 hr and restained for a further 48 hr with fresh stain; one gel was stained with SYPRO Ruby protein gel stain (image not shown). The gels were destained with and stored in water. Gel images were acquired on an Agfa Arcus II scanner throug...

The Bio-Rad Silver Stain, Rev E

...staining was first introduced as a general protein stain useful in polyacrylamide gel analysis i...l. 54 This first practical PAGE silver stain was of the silver diammine type adapted from early...ore reliable, and very sensitive silver stain derived from a photographic chemical process. ...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

...elative quantitation accuracy is often highest for proteins that stain with similar efficiency to the upper marker protein. B...discussed previously. Thus, a protein with poor affinity for the stain would have to be present at concentrations above 2.5 ng/l to be ...

Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C

...isease (Figures 4C, F, I, L, and O) obtained from a patient with invasive disease. As depicted in the figure, the tumor does not stain with keratin 19 antibodies (Figure 4B), is BG3C8-antigen positive (Figure 4E) (Celis et al. 1996b), keratin 13 negative (Figure ...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

... volts until the dye front reached the bottom of the gel. Following electrophoresis, the gel was silver stained using the Silver stain Plus kit (Bio-Rad). DNA POLYMERASE α ASSAY DNA polymerase α activity was assayed with an activated calf thymus DNA ...

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

...g Protocol Note: This protocol must be followed rigorously to obtain optimal results. 1. Following electrophoresis, stain the gel with 1.0 g/ml ethidium bromide (EtBr) for exactly 30 minutes with constant agitation. Use a fresh dilution of EtBr for e...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

...00 constant volts and at 60 C, constant temperature. Individual bands on the parallel DGGE gels were visualised using SYBR-Green stain and UV illumination. Results and Discussion A scheme for identifying HLA-DRB alleles using DGGE is shown in Figure...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

... 10% polyacrylamide resolving, 3% stacking gel using the Mini-PROTEAN II slab cell (Figure 2). Gels were stained using the Bio-Rad Silver stain Plus kit. Results Chlorella sorokiniana NADP-specific GDH was purified an additiona...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

... the genomic DNA was extracted from paraffin embedded materials. In such cases we used SYBR Green I instead of ethidium bromide to stain the gels. This improved sensitivity and enabled us to see the bands clearly. These experiments indicate that DGGE is a p...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

...igure 1A). Fractions 913 were pooled and concentrated. The presumed M-protein was electrophoresed and detected by Coomassie blue stain (Figure 1B, lane 5) and its virus specificity was confirmed by western blot (Figure 1C, lane 5). After concentration of fraction...

Protect RNA and Improve Target Cell Identification

...nuclei, and nucleoli. This histological stain has been used to stain neurological tissues and is especially useful ...n) Acridine Orange stain intercalates into nucleic acids to enha...

Fast, Accurate Assessment of siRNA-induced Gene Silencing

... qRT-PCR with SYBR Green Detection SYBR Green is an economical nucleic acid stain that binds to double-stranded DNA and can be used to detect PCR products on most real-time detection platforms. ...

Drive Long-Term Silencing with CMV Vectors

...y and the resulting reduction in protein expression was examined by fluorescence microscopy. The cells were mounted with DAPI to stain nuclei (Blue). Images were digitally captured and quantified using Metamorph software. The p Silencer 4.1...

Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA

... interpret and the chip runs on the bioanalyzer are less time consuming. It can take up to an hour and half to prepare the samples, run a gel, stain the gel, and take a picture. The whole Agilent process (sample preparation to saving the file) can be done in half that time. There is no impr...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

...patible with the RNA Pico Assay, but significant differences in RNA yield and quality were observed. Best results were obtained using the MG stain (average yield: approx. 3100 pg; highly intact RNA; see fig. 3A). HE (figure 3B) and NFR staining (figure 3C) gave very similar results in ter...

Comparison of different protein quantitation methods

...s Deutschland GmbH (Waldbronn, Germany). All SDSPAGE reagents and gels were purchased from Invitrogen GmbH (Karlsruhe, Germany). Coomassie stain Solution and Destain Solution were purchased from BIO-RAD Laboratories GmbH (Munich, Germany). The digital camera and the imaging software w...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

...ye loading buffer after harvesting. 2. stain cells with calcein (up to 5 x 106/ml, 0.5 M calc... 3. Wash once in staining buffer. 4. stain cells (1 x 106) in a total volume of 100 l (80 l...nd centrifuge (500 x g, 5 minutes). 7. stain cells (1 x 106) with GR antibody in a total volu...

Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining

...he exact adjustment of cell concentration and density of samples with few cells. Cells were transfected and stained on-chip with the live cell stain CBNF as described in reference 2. Only 30,000 cells were required for onchip staining of primary cells and subsequent measurement on the 2100 ...

Optimizing electroporation parameters

...ons. The easiest way of doing so is by incubating the cells for 30 minutes in hypoosmolar buffer and then performing a viability stain using trypan blue or propidium iodide. If observation under a microscope reveals lysis in more than 10% of the cells, the osmolarity ...

High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

...ded satisfactory efficiencies. Twenty-four hours after transfection, we used Stratagenes in situ b-galactosidase staining kit to stain cells with X-gal, and the resulting cells that expressed for b-galactosidase were photographed ( Figure 2 ). ...

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

...ure 1 Figure 1 shows a gel as imaged by the system using either the ethidium bromide (panel A) or silver stain (panel B) optical filter with transillumination. Figure 2 shows an image of the gel obtained using the same filters but with ...

hNT BlueNeurons Express b-Galactosidase at High Levels

...ons hNT BlueNeurons have high levels of beta-galactosidase activity and stain dark blue in standard assays. This feature is especially useful for transplantation studies of CNS tissues, where the ability to stain for beta-galactosidase activity allows hNT BlueNeurons to be easily disting...

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

...Filter, an optical bandpass filter designed for SYBR Green nucleic acid stain and FLASH CAT applications. 1 The Evergreen Filter maximally overlaps ...ye. Proteins stained with Coomassie blue appear blue in color because the stain absorbs non-blue wavelengths of visible light while reflecting blue light...