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siRNAs in Biological Technology

Using Validated siRNAs in Functional Genomic Assays

Collections of siRNAs targeting key human genes are ideal for studying protein function in cells. To identify genes involved in a given cellular process, cells can be transfected with different siRNAs and assayed f...

Test More siRNAs for Less

No longer must you endure the high cost and long turn-around times required for chemically synthesized siRNA. The new Silencer siRNA Construction Kit (patent pending) saves you both time and money so now you can expand ...

Optimize Transfection of siRNAs for RNAi

By Rich Jarvis, Associate Scientist Low transfection efficiency is the most frequent cause of unsuccessful gene silencing experiments. We have found that by optimizing cell density, transfection agent amount, and siRNA concentra...

Deliver siRNAs Into Primary Cells

siPORT siRNA Electroporation Kit Deliver siRNA into primary cells, cells grown in suspension, and other difficult-to-transfect cells Maintain high cell viability under a wide ...

Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating the results by real-time RT-PCR. Ambion's Silencer Validated and Silencer Pre-designed...

Delivering siRNAs to Difficult Cell Types

Electroporation of Primary, Neuronal and Other Hard-to-transfect Cells Dmitriy Ovcharenko, Rich Jarvis, Kevin Kelnar, David Brown, Ambion, Inc. RNA interference (RNAi) is a powerful exp...

IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

High Quality Custom Synthesis of siRNAs Cenix Pre-designed siRNAs to Human, Mouse, and Rat Genomes Silencer Validated siRNAs Silencer siRNA Libraries ...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

Dmitriy Ovcharenko (Research Associate II, Ambion, Inc.) Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells ...

Cenix-Designed siRNAs for 95% of Human and Mouse Genomes

siRNA designs now available for >95% of all human and mouse genes listed in RefSeq Design algorithm developed by Cenix results in highly effective siRNAs High quality custom siRNA synthes...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the Silencer siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA ...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most c...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most c...

Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs

The Silencer siRNA Construction Kit uses an in vitro transcription reaction and column purification to prepare transfection-ready siRNAs. With this kit, multiple siRNAs can be prepared in ~24 hours. Below ...

RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs

David Brown (Senior Scientist, Ambion, Inc.) The realization that RNA interference (RNAi) can be used as a general tool for mammalian gene function studies occurred with the seminal Elbash...

Optimizing Chemical Transfection and Electroporation of siRNAs

Rich Jarvis (Associate Scientist, Ambion, Inc.) Alan D. King (Vice President and CSO, Cyto Pulse Sciences, Inc.) Low transfection efficiency is the most frequen...

Five Ways to Produce siRNAs

Choose the Best Method for Your Research Many researchers now use small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Here we describe five methods for producing s...

Silencer Validated siRNAs

Silencer Validated siRNAs are single siRNA duplexes that have been verified by real-time RT-PCR to reduce the expression of a number of important human genes. The cut-off for siRNA validation is a >70% reduction in target gene expression 48 hours post-tra...

Quark Pharmaceuticals Presented At ARVO Data Showing That PF-04523655 Enters Retinal Cells And Elicits Its Pharmacologic Effect Via Target Gene Knock-Down Without Activating TLR3

...ity of Kentucky , member of Quark's Medical Advisory Board, and one of the authors on the study, stated, "My laboratory's research has shown that many sirnas suppress neovascularization regardless of their sequences or targets and can have dangerous off target effects. For that reason, I'm gratified to see ...

Sigma-Aldrich Introduces MISSION(R) esiRNA for Low Cost, High Specificity Pooled RNAi Screening

...iRNA ( http://www.sigma.com/esirna ) incorporates a pool of hundreds of sirnas against a single gene target. This new tool is designed to eliminate the tr...esiRNA library at ICCB-Longwood." esiRNA are endoribonuclease-prepared sirnas synthesized by in vitro transcription of a 300-600-bp gene specific doubl...

Roche Madison Moves to New Facility

...ble targets and has great potential for many diseases. DPCs represent a first in class polymer-based nanoparticle that allows for targeted delivery of sirnas to various cell types in the body. A bout Roche Hoffmann-La Roche Inc. (Roche), based in Nutley, N.J., is the U.S. pharmaceuticals headquart...

Quark Pharmaceuticals to Present at American Society for Pharmacology and Experimental Therapeutics Annual Meeting

...lam Pharmaceuticals (topic: Progress in developing sirnas as drugs); and Patrick L. Iversen, Senior Vice Pre... a number of on-going clinical trials of synthetic sirnas in various disease indications, status as the firs...s, an, enabling portfolio of IP covering synthetic sirnas and validating partnerships with companies such as...

Sigma-Aldrich Introduces MISSION(R) In VIVO QUALITY and iScale Oligos(TM) siRNA, a Superior Quality siRNA Designed for RNAi Research in Animal Models

... "Sigma-Aldrich's MISSION(R) In VIVO QUALITY sirnas are used exclusively at BIOO Scientific(TM) for al...) Corporation. "Most recently, we have used these sirnas to with great success to demonstrate the utility o.... BIOO Scientific(TM) Corporation recommends these sirnas to our all of our customers as they are exceptiona...

Intradigm Licenses Key Next Generation RNA Interference (RNAi) Intellectual Property From University of Massachusetts Medical School

...IP includes parameters for the structural modifications for next generation sirnas including the "Zamore Design Rules" that significantly improve the potency ... of the RNAi NPX delivery technology is its ability to incorporate multiple sirnas in a single drug product. These multi-targeted therapeutics can simultaneou...

Self-assembled materials form mini stem cell lab

...tor important in the signaling of stem cells -- could cross the membrane, and that the stem cells were able to differentiate. We expect that genes, sirnas and antibodies will cross the membranes as well, making this mini cell biology lab a powerful device for research or therapies, said Stupp. For the de...

OPKO Health Announces Issuance of U.S. Patent Covering the Use and Administration of Certain Anti-VEGF siRNA's, Including Its Phase III Compound Bevasiranib

... the first siRNA to demonstrate clinically relevant activity in patients, the first siRNA to enter a Phase III pivotal trial and now, one of the first sirnas to receive a U.S. patent covering its broad therapeutic use. This second issued patent covering bevasiranib and its anti-VEGF applications further rei...

Nastech Assigns Intellectual Property Related to the Development of RNA-Based Therapeutics to its Subsidiary, MDRNA, Inc.

... RNA interference, or RNAi, is a cellular mechanism that can be used to turn off the production of a protein by using small interfering RNAs (siRNAs). sirnas are double-stranded RNA molecules that are able to silence a gene in a sequence-specific manner by degradation of the target messenger RNA. In the cas...

Mechanism for the in-vivo transport of siRNA

...ticular the administration of small RNAs, known as sirnas (small interfering RNAs), in animals proved difficult. Although it was possible to administer sirnas successfully by using various methods such as high...Stoffel and his team turned to chemically modified sirnas in combination with cholesterol, not because the m...

FlexiPlate siRNA

... genes. For maximum flexibility, select sirnas and specify plate layout at the GeneGlobe Web ... siRNA: Maximum flexibility select sirnas targeting human and mouse genes, contro...eriments to suit specific requirements. sirnas for any human or mouse gene can be selected as wel...

Measuring siRNA-mediated knockdown of IL-8 mRNA

...horbol-12-myrstyl-13-acetate (PMA, Calbiochem, EMD Biosciences, San Diego, CA, USA) in SFM media (Gibco Cell Cultures, Invitrogen) for two hours. The sirnas specific for the IL-8 mRNA (Trilink Biotechnologies, San Diego, CA, USA) were diluted in OptiMEM media (Invitrogen) to a final concentration of 100 nM...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

...P) ◊ cell cycle sensors to screen a library of sirnas directed against key cell cycle control genes. Ima... detailed description of the phenotypic effects of sirnas in cellular screens.

In this st... cell cycle sensors (Fig 1) to screen a library of sirnas directed against key cell cycle control genes. ...

RNA Interference and Gene Silencing: History and Overview

...earchers studying C. elegans. sirnas - Small interfering RNAs. Curre... nucleotide dsRNAs mediate PTGS. Introduction of sirnas can induce PTGS in mammalian cells. sirnas are apparently produced in vivo b...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

...chers at the NIH, use PAGE purified sirnas from Ambion to study gene function .... Chemically synthesized, PAGE purified sirnas (Ambion) were transfected into cells grown to 4070...in complete medium without antibiotics. Individual sirnas (2080 nM) were mixed with Oligofectamin...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

...some non-mammalian organisms, amplification of the sirnas is thought to take place by an RNA-... of small molecules in and between cells. However, sirnas must interact with cellular compone...d negatively affect the capacity of sirnas to induce gene silencing. We prepared fluorescentl...

Superior Gene Silencing Using Adenoviral Vectors

... recombinant adenovirus expressing sirnas has only recently been descri... injected recombinant adenoviruses expressing sirnas targeting green fluorescent protein (GF...specificity, a potential application of sirnas is the treatment of dominantly inherited diseases....

siRNA Expression Vectors with Selectable Markers

...RNA interference pathway with sirnas (see Silencer Validated sirnas to Stat-1, p53, and PCNA; and Silencer GAPDH control sirnas for eliciting RNAi. Also used were ...
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