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Easily Amplify Genomic DNA with Long-Distance PCR

Long DNA polymerase to genotypemice Cardiovascular Research Center, University of Cincinnati, Cincinnati, OH We developed a simple, reliable procedure to genotype mice usinglong-distance PCR, whereby genomic DNA is isolated from mouse ear clips, an...

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

Optical Filter Optimized for Imaging Silver-StainedGels Stratagene II Still Video System that increases contrast...

Printable CELLocate Log Sheets for Easily Locating Adhererent Cells

Log Sheets for Easily Locating Adhererent Cells CELLocate is the ideal solution for relocating adherent cells that have been used for microinjection. These sterile coverslips come with a microgrid pattern. Using the CELLocate proto...

Custom and library siRNA for efficient gene silencing

High-quality short interfering RNA (siRNA) from QIAGEN allows efficient gene silencing in eukaryotic cell culture by targeting complementary endogenous mRNA. This effect, termed RNA interference (RNAi), enables targeted gene silencing in functional genomics studies. Now, QIAGEN offers a custom siRNA oligonucleotide syn...

Custom and library siRNA for efficient gene silencing

High-quality short interfering RNA (siRNA) from QIAGEN allows efficient gene silencing in eukaryotic cell culture by targeting complementary endogenous mRNA. This effect, termed RNA interference (RNAi), enables targeted gene silencing in functional genomics studies. Now, QIAGEN offers a custom siRNA oligonucleotide syn...

RNAi Gene Silencing System

Gene silencing by RNA interference (RNAi) in eukaryotic cell cultures requires double-stranded molecules known as short interfering RNA (siRNA). QIAGEN is a licensed supplier of siRNA, offering a custom siRNA oligonucleotide synthesis service , <a href="http://www.biocom...

Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*

Center for Neurologic Diseases, Brigham and Womens Hospital, Harvard Medical School, Boston, MA, USA Genetic manipulations using neuronal cells are notoriously difficult. Therefore, efficient delivery of short interfering RNA (siRNA)...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

One of the RNA silencing products available from Ambion is a kit for the synthesis of siRNA, the <A href="http://www.ambion.com/catalog/CatNum.php?1620">Silencer <A href="http://www.ambion.com/catalog/CatNum.php?16000"> siRNA Construction Kit . While this kit is very cost effective...

RNA Interference and Gene Silencing: History and Overview

May 20, 2002 Post-transcriptional gene silencing (PTGS), which was initially considered a bizarre phenomenon limited to petunias and a few other plant species, is now one of the hottest topics in molecular biology (<A href="#1">1 ). In the last few years, it has become clear that PTGS occurs in both plants...

Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit

Although the <A href="http://www.ambion.com/catalog/CatNum.php?1632"> siRNA Labeling Kits were developed for labeling siRNA, they can be readily adapted for labeling long dsRNA. The following protocol can be used for this application. See "<A href="#visualizing">Visualizing In...

Silva-Craig joins Neurognostics board

Neurognostics, which spun out of the Medical College of Wisconsin in 2003, develops magnetic resonance imaging (fMRI) products and services Silva-Craig is the president and chief operating officer of Ema...

Silicon Logic Teams Up to Save Money

Eau Claire, WI-- The business collaboration is meant to speed...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

Since the first description of RNA interference (RNAi) asa protection mechanism against invasion of foreign genesappeared a few years ago, RNAi has become a powerfultool for analyzing gene function in mammalian cells. RNAiis mediated by short double-stranded RNAs that a...

Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection

In addition to the specificity andefficiency of an siRNA sequencefor a target gene, which are ofhigh importance in post-transcriptionalgene silencing (PTGS), thedelivery of siRNA into cells is crucial.An efficient method to optimizetransfection and gene silenci...

Silencer Validated siRNAs

Silencer Validated siRNAs are single siRNA duplexes that have been verified by real-time RT-PCR to reduce the expression of a number of important human genes. The cut-off for siRNA validation is a >70% reduction in target gene expression 48 hours post-transfection, though the average reduction in target gene expression in the inital set...

Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation

Quantitative or real time RT-PCR is one of the most commonly used techniques for quantitating changes in gene expression across samples. When working with cell or tissue samples for subsequent use in RT-PCR you must extract...

GENE SILENCING WITH PCR PRODUCTS

siRNA expression vectors offer enormous opportunities to study gene silencing. Unfortunately, the preparation of siRNA vectors is labor and time intensive. Ambion's new Express siRNA Expression Cassette Kits pr...

IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

High Quality Custom Synthesis of siRNAs Cenix Pre-designed siRNAs to Human, Mouse, and Rat Genomes Superior si...

Drive Long-Term Silencing with CMV Vectors

Ambion's newest line of plasmid siRNA expression vectors, p 4.1-CMV, feature polymerase (pol) II promoters that drive transcription of both an siRNA template and an antibiotic resistance gene. The CMV promoter is used to drive siRNA expression. This...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

4.1-CMV plasmid vectors are designed for long-term gene silencing experiments in a broad range of cell lines. The vectors carry an RNA polymerase II-type CMV promoter (human cytomegalovirus immediate-early promoter) and an optimized SV40...

Enhanced siRNA Delivery and Long-term Gene Silencing

5.1 Retro System enables researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. These retroviral vectors make use of H1 or U6 Polymerase III promoters...

Fast, Accurate Assessment of siRNA-induced Gene Silencing

Cells-to-Signal Kit: Small Sample Prep for qRT-PCR Real-time, quantitative RT-PCR (qRT-PCR) is the most sensitive way to detect and quantitate mRNA and is often used to validate techniques that monitor changes in gene expression, including array analyses and RNA...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

The first step in preparing a plasmid for siRNA experiments is to identify target sequences in the gene of interest that are susceptible to siRNA-induced degradation. We have found that a little more than half of the siRNAs provide at least a 50% reduction in target mRNA levels and approximately 1 out of 4 siRNAs...

Superior Gene Silencing Using Adenoviral Vectors

Mediating RNA interference (RNAi) with recombinant adenovirus expressing siRNAs has only recently been described in the literature (1). Recombinant adenoviruses have several advantages over other methods of nucleic acid delivery. Namely, these viruses can infect dividing and...

Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

Iezzi M, Kouri G, Fukuda M, Wollheim CB (2004) Synaptotagmin V and IX isoforms control Ca J Cell Sci In a recently published paper in the Journal of Cell Science, Iezzi and colleagues used Ambions...

Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs

siRNA Construction Kit uses an in vitro transcription reaction and column purification to prepare transfection-ready siRNAs. With this kit, multiple siRNAs can be prepared in ~24 hours. Below is one example of how siRNAs prepared by the Silenc...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most common m...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most common m...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. Here, resear...

The Bio-Rad Silver Stain, Rev E

Silver staining was first introduced as a general protein stain useful in polyacrylamide gel analysis in 1979 by Merril et al. This first practical PAGE silver stain was of the silver diammine type adapted from early histological silver stains. In 1981, Merril et al. introduced...

Silver linings in the cloud: America's dearth of STEM students

It's even more impressive that their competitors were seasoned college students. Led by instructor Timothy Jump, students from the Mi...

What makes Silicon Valley so different?

, getting a first look at some very interesting technology start ups. (Yep, you guessed it, the search for DEMO 2007's demonstrating class is in full swing.) Much of what I saw was quite impress...

New Silicon Pastures director envisions more home cooking for Wisconsin angels

While others are talking about brain drain and t...

Anti-ethanol arguments are easily debunked

- There has been a great deal of media coverage on biofuels and ethanol, much of it confusing. I want to save you the trouble of reading all this confusing information, so let's straighten out all the...

Canadian firm acquires Silicon Logic

SLE will continue to be b...

Study explains unexpected conductivity of nanoscale silicon

When graduate student Pengpeng Zhang successfully imaged a piece of silicon just 10 nanometers-or a millionth of a centimeter-in thickness, she and her UW-Madison co-researchers were puzzled. According to established thinking, the feat should be impossible because her microscopy method required samples that conduct electricity. "After she did it, we realized, 'Hey, this silicon layer is...

Silent partners: Selecting a web hosting company

An important part of doing business online is finding the right web host - the company that provides you with the server space, database and internet connectivity yo...

Silicon Logic Engineering Ramps Up Custom Chips

Eau Claire, Wis.In a business world where even very specialized services and products quickly become commodities, The Eau Claire-based company specializes in design and verification of custom processing chips for h...

Silicon Logic Enables High-Speed Communication

Jeff West has done both, and in the process has poised veterans Mike Berry and Bob Solberg, to sit on the crest of the high-speed communications wave already taking shape on the high seas of communica...