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New Microarray Labeling Kit for Preparing cDNA Probes

A more reliable and reproducible way to create fluorescently orradioactively labeled cDNA David T. Wong Heidi Davis Darryl Garrison TerryPayette Matt Lundy Kim Kuplent Mary A. Buchanan Joseph A. Sorge Rebecca L. Mullinax Stratagene Phenogenex Nucleic acid micro...

Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications

Herculase enhanced DNA polymerase delivers high fidelity and greatperformance Stratagene Stratagene has recently released Herculaseenhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Compariso...

Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes

New GeneConnection discovery microarrays provide more than 4,000 uniquehuman cDNA sequences Stratagene introduces the first in a series of human cDNA microarrays on glass slides. These GeneConnection discovery microarrays which are based on the technology published by Schena et al., can be...

An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat

GBF Gesellschaft fr Biotechnologische Forschung mbH (Society for Biotechnological Research) DNA microarrays are a key technology in modern molecular biotechnology. Due to their high degree of parallelism, they allow the...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

BIOGEM, Division of Biology, University of California San Diego, La Jolla, CA 92093-0349 e-mail: ghardiman@ucsd.edu www.biogem.ucsd.edu Microarray technology, primarily advanced by Pat...

Critical factors for successful microarray and real-time PCR analyses

QIAGEN GmbH, Hilden, Germany Gene expression profiling at the RNA level has been significantly facilitated by microarray analysis and quantitative real-time RT-PCR. Microarray analysis offers the advantage of profiling expression levels of hundreds or thousands...

Acrylamide Polymerization A Practical Approach

Paul Menter, Bio-Rad Laboratories, 2000 Alfred Nobel Drive,Hercules, CA 94547 USA The unparalleled resolution and flexibility possible with polyacrylamide gel electrophoresis (PAGE) has led to its widespread use for the separation of proteins and nucleic acids. Gel porosity can be varied over a...

National broadband policy: Let's get it right this time

Several articles have popped up that discuss the need, but do not reflect some of the keys to making it right. One <a href="http://thehill.com/thehill/export/TheHill/News/...

Madison-based TrafficCast makes national inroads as data provider

, a company that provides traffic data to a range of other map and travel services, is expanding well beyond its Madison headquarters in order to meet with the demand from i...

Broadlook narrows search for sales leads with software

What's more, the founder and CEO of Pewaukee-based might have just the silver bullet to do it. Using Broadlook's primary software suite Profiler, Eclipse and Contact M...

Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)

As the human genome sequence project is completed,the vast amount of genomic information calls formethods that allow gene expression analysis on agenomic scale and speed up detection of significantsequence variations. The most suitable tools available forgene express...

Use of Transcriptor Reverse Transcriptase in Microarray Analysis

Researchers studying tissue and cellular differences in gene expressionhave applied microarray analysis to questions ranging from cancer researchto plant development studies. Although the method is well established, manyof the steps in the process continue to be optimised...

Increased Sensitivity in Microarray Analysis

Transcriptor Reverse Transcriptase inthe Eberwine Target Preparation Workflow Reproducibility and sensitivity are key parameters for thequality of a gene expression profiling experiment. Theimproved reliability of commercially avail...

Analysis of Microarray Data

and aRNA Samples Clustering analysis is commonly used for interpreting microarray data. It provides both a visual representation of complex data and a method for measuring similarity between experiments (gene ratios). The widely used methods for clustering microarray data are: Hierarchical, K-means and...

Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform

Ambion has developed a complete line of research tools for the analysis of microRNAs (miRNAs). Recently, we introduced the Vana miRNA microarray platform (consisting of the Vana miRNA Labeling Kit and the Vana miRNA Probe Set) and several miRNA tools for interrogating miRNA function and identifyin...

Is RNA Amplification Necessary for Microarrays?

RNA amplification using the Van Gelder and Eberwine technique (1) has been used extensively for target synthesis and labeling in array analysis experiments. Amplification of RNA is clearly necessary when working with limited samples (needle biopsies, LCM samples, etc.), but is...

Perform Microarray Analysis with Limited Bacterial RNA

Linear RNA amplification technology for bacterial gene expression analyses As little as 10 ng of mRNA or 100 ng total RNA required for array analysis Low input requirements enable analysis of mixed host-pathogen samples Ful...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

RNAqueous-Micro Kit Ideal for Processing Laser Capture Microdissection Samples Further development of Ambion's RNAqueous-Micro Kit has resulted in a 3-6 fold increase in total RNA yields from LCM samples. In addition, Ambion has introduced a new LCM Staining Kit that has been o...

High Throughput RNA Isolation for High Performance Microarray Analysis

Protocol combines benefits of TRI Reagent sample lysis with MagMAX magnetic bead-based RNA isolation technology Procedure is compatible with fresh, flash-frozen, or RNAlater-treated tissues...

Preserve Samples for RNA Expression Microarrays RNAlater Around the World

protects cellular RNA within specimens during sample collection and during shipping. Whether you are in the lab, in the OR, or out in the field, you can use this aqueous, non-toxic Tissue Collection:RNA Stabilization Solution from ambient temperatures down to...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

, and Scott E. LaPatra 1 Department of Fish and Wildlife Resources, University of Idaho, Moscow, ID USA 2 Clear Springs Foods Inc., Research Division, PO Box 712, Buhl, ID USA Coldwater disease (CW...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

<P >J. Turner GE Healthcare, The Maynard Centre, Cardiff, UK Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ Kinase Assay Kits gene...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

Se-Jin Yoon and Kyung-Ah Lee Infertility Medical Center, CHA General Hospital, Seoul 135-081, Korea Laser Capture Microdissection (LCM) is an innovative technique which permits the rapid and reliable procurement of pure cell populations from heterogeneous tissue sections. After LCM, RNA isolated from pure cell populations was amplified to generate a...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

This application note describes a method for extracting and isolating total RNA from rodent tissues that have been fixed in formalin and embedded in paraffin, and the subsequent amplification of mRNA from these tissues for use in microarray experiments. We have used this methodology to demonstrate that robust and reliable methods do exist for retrieval of...

Simple Approaches for Optimization of RT-PCR

RT-PCR is a powerful tool for analysis of gene expression and for characterization of RNA splice variants. RT-PCR is generally carried out as either one-step RT-PCR, in which all reagents are combined in one tub...

New Tools for Making and Processing Protein Microarrays.

The use of DNA microarrays in scientific research and drug discovery has become very widespread. However, since it is proteins and not genes that are the vast majority of the true drug targets, there...

Kind approach to leveraging biofuels

Like many farmers in Wisconsin, th...

Techware to extend broadband wireless network to Butler

The company, which started the build out in Germantown, said it will begin to deliver broadband service that is capable of providing speeds up to 14 <a href="http://en.wikipedia.org/wiki/Mega...

Purchase of Madison broadband network may bring improved service

, according to a report in the <a href="http://www.madison....

Verizon expands broadband service in Wisconsin

According to Verizon, customers will be able to upload fi...

Carlini beats drum for high-speed broadband

To Carlini, president of , moving from dial-up Internet service to DSL is like adding vinyl to the top of a stagecoach. Why bother when there is a <a href="ht...

Techware revives broadband project

Techware, which provides computer consult...

Meier joins Broadlook Technologies as COO

Meier brings experience in the legal, private equity, and venture capital sectors, and he will be responsible for helping the Milwaukee-based Broadlook grow through sales, marketing, and...

Wait `till next year OK for baseball, not broadband

There should be as m...

Ethanol producers are at a crossroads

Public ethanol companies recently have seen trading multiples in excess of $5 per gallon of estimated 2007 an...

Broadband blues: Don't fall for Lightspeed hype

In the great race for global competitiveness, economic development equals broadband connectivity and broadband connectivity equals jobs. The fuel for broadband connectivity is the need for speed. Thats all the consumer need...

Rural company works to improve broadband

, a satellite-based broadband acce...

NimbleGen microarrays speed genetic findings

Researchers at the discovered new genetic causes of mental retardation, document...

At 30, biotech is an industry at a crossroads

In this a...

Municipal broadband in Illinois: Where are the standards?

Quinn had some encouraging remarks about keeping the playing field open f...