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Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted

Quantitating cell number is more accurate using the Quantos cellproliferation assay kit Genetic Applications LLC cell proliferation assay kitprovides a simple, reproducible method to assess the number of cells present invarious tissue culture dish formats. In this st...

Sample Preparation for Microinjection

Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 m [1]. This capillary is back loaded with the sub...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as quickly as possible are adapted from Eppendorf Brochure...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume

by Varying the Final Elution Volume Eppendorf 5 Prime, Boulder, Colorado Perfect gDNA Blood Mini Kit protocol recommend...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as quickly as possible are adapted from Eppendorf Brochure...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume

by Varying the Final Elution Volume Eppendorf 5 Prime, Boulder, Colorado Perfect gDNA Blood Mini Kit protocol recommend...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompar...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocomp...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompar...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany The classic RNA isolation procedures are...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

Stefanie Schrer, Werner Wittke,* and Christian Kupfer QIAGEN GmbH, Hilden, Germany * Leica Microsystems, Wetzlar, Germany Laser microdissection (LMD) provides a powerful technology to isolate specific cells or cell groups for further analysis....

Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer

Nicola Hughes, Jean-Jacques Dunyach, 1Biovail Contract Research,Toronto,Ontario,Canada; 2Thermo Finnigan,San Jose,CA,USA; 3Therm...

Genomic DNA Preparation from RNAlater Preserved Tissues

The following protocol is suitable for 100 mg tissue samples. Mouse kidney, lung, and spleen genomic DNA have been successfully extracted with approximate yields of 70 g DNA per 100 mg tissue....

Separation Reproducibility With the BioLogic Chromatography Systems

Kevin Thornton, PhD, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA In chromatographic separations, sample component elution times are often used both for identification and to program automated chromatography steps like fraction collection. Therefore, the ability to obtain reproducible...

Cell Proliferation Assays

The cell proliferation assay is an important tool in the assessment of the immune system function and in the search for new drugs. Productivity with this technique has been limited by the lack of automated, high throughput sample processing and counting equipment. The MicroMate harvester and the TopCount Microplate Scintillation Counter help r...

Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)

Calibration of a dissolution apparatus is necessary to produce accurate results for dissolution testing of pharmaceutical dosage forms and to allow comparison between batches and between laboratories. USP requirements for calibration are given in the Apparatus Suitability Test in USP Each individual appa...

Rockwell Q2 operations net income soars, shares plunge

Income from continuing operations at Milwaukee-based Rockwell Automation Inc. for the quarter ended March 31 rose to $142.5 million, a 90 percent leap from the $74.9 million the company earned the same quarter of 2004. Profit for the second quarter of 2005, including discontinued operations, was an even $150 million, compared to $78.3 million a year ago. Despite that, Rockwell s...

Biotech industry reaches midpoint in 50-year maturation cycle

An interesting article in ) on March 1 states unequivocally that most industry cycles run about 50 years until they mature. If this...

Big collaboration links learners to science on a tiny scale

In the classroom, st...

Digital River Provides E-Commerce Operations for Metrowerks

MINNEAPOLIS-Digital River, Inc. a e-commerce outsource provider, today announced that it has signed an agreement with Metrowerks, aprovider of software development tools and services. Based on the agreement, Digital River built Metrowerks' e-commerce site, located at store.metrowerks.com, to support the online sales and marketing of Metrowerks' line of CodeWarrior(TM) software and hardware produc...

DEMO 2003 Closes with Focus on Collaboration and Communication

Scottsdale, AZ - DEMO 2003 continued to buzz with innovation and excitement on its closing day, focusing on a myriad of new technologies that included timely next-generation systems designed to protect and defend the national IT infrastructure as well as collaboration tools and evolving email services. In addition to an array of new product demonstrations, technology leaders discussed the possibi...

Japan, Midwest Biotechnology Industry Leaders Identify Steps for Greater Collaboration Between Countries

CHICAGO,IL--Top industry, government and academic leaders in the biotech and life science industries from the Midwest U.S. and Japan gathered Wednesday in Chicago as part of the JETRO Midwest-Japan Biotechnology Summit. The topics covered similarity of challenges faced by Midwest Biotech companies and their counterparts in Japan. The goal was to foster discussion and lay down specific p...

Trouble-shooting: Misincorporation or low fidelity

Here are some troubleshooting hints that we have gathered regarding misincorporation or low fidelity of PCR reactions: <a href="#Option1">Magne...

Roche Diagnostics Corporation

Advantages of Roche Applied Science amplification products <a href="http://w...

High Pure PCR Template Preparation Kit

For isolating genomic nucleic acids for PCR, restriction enzyme analysis, and Southern blotting Catalog Number Pack Size 1796828 1 kit (100 purifications)...

General Considerations for Successful Transfection Experiments

Keep an eye on your cells; ensure they are in good condition and set a suitable plating protocol for optimal cell density from the beginning to the end of transfection....

Roche Diagnostics Corporation

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Ampl...

Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

The endopeptidase originally isolated from the filtrate ofthe lower fungus Tritirachium album limber is recombinantlyexpressed in Pichia pastoris. Proteinase K isextremely effective on native proteins from differentsources, and remains active in the presence of denaturingreag...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...

Cell Proliferation and Viability Measurement

The quantification of cellular growth, including proliferationand viability, has become an essential tool in anylaboratory working on cell-based studies. Such techniquesenable not only the optimization of cell cultureconditions, but also the determination of growth factorand...

Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B

Siobhan Pickett, M.S., Sean Carriedo, Ph.D. and Chang Wang, Ph.D. Axon Instruments, Inc. 3280 Whipple Road, Union City, CA 94587 Last updated: September 13, 2001 An important criterion for comparing the...

measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

Cathy Olsen, Ph.D., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089....

Dicer vs. RNase III for Preparation of siRNA Cocktails

Both recombinant human Dicer and RNase III can be used in vitro to cleave long dsRNA, producing siRNA cocktails that are effective mediators of gene silencing (1-6). Dicer is an endoribonuclease that contains RNase III domains and is the enzyme responsible...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

(Research Associate II, Ambion, Inc.) Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells are often desired for such experiments because they are mor...