PCR in Biological Technology

New White Paper Examines Advanced Mycoplasma Tests - How Real-Time PCR Analysis Helps Save Time and Money for Biopharmaceutical Manufacturers

AGAWAM, Mass., March 16 /PRNewswire/ -- Contamination by mycoplasmal organisms is an ever-present concern in biopharmaceutical manufacturing. Their properties make them ready sources of contamination, while rendering them difficult and time-consuming to detect. A new free white paper, examines how...

WaferGen to Present Data on SmartChip(TM) Real-Time PCR System at Cambridge Healthtech Institute's Quantitative PCR Conference

FREMONT, Calif., March 10 /PRNewswire-FirstCall/ -- WaferGen Biosystems, Inc. (OTC Bulletin Board: WGBS), a leading developer of state-of-the-art genetic analysis systems, today announced that Kumar Kastury, Ph.D., WaferGen's vice president of research and technical operations, will present data o...

Data on WaferGen's SmartChip Real-Time PCR System(TM) to be Presented at 16th International Molecular Medicine Tri-Conference

FREMONT, Calif., Feb. 23 /PRNewswire-FirstCall/ -- WaferGen Biosystems, Inc. (OTC Bulletin Board: WGBS), a leading developer of state-of-the-art gene expression, genotyping, cell biology and stem cell research systems, today announced that the validation results of the company's SmartChip Real...

NIH Awards Approximately $3M Grant for Research Involving WaferGen's SmartChip(TM) Real-Time PCR System at the University of Pittsburgh

Research Effort Includes Development and Application of PulmoSmartChip - A Custom Molecular Phenotyping Assay for Lung Disease FREMONT, Calif., Oct. 30 /PRNewswire-FirstCall/ -- WaferGen Biosystems, Inc. (OTC Bulletin Board: WGBS), a leading developer of state-of-the-art gene expressio...

Ahram Biosystems Completes Development of a Palm-Size Portable PCR Device

New instrument system delivers highly efficient and fast PCR amplification in a fully portable format SAN DIEGO, June 17 /PRNewswire/ -- Ahram Biosystems, Inc., a leading developer of state-of-the-art PCR devices and Cell-based assay systems, today announced at the BIO 200...

Enigma Signs Real-Time PCR Licences With Applera

PORTON DOWN, England, February 7 /PRNewswire/ -- Enigma Diagnostics, the life sciences company developing rapid molecular diagnostics systems, announces today that it has signed license agreements with Applera Corporation. The licenses, signed 6 February 2008, include a Real-Time Instrument ...

Rosetta Genomics Selects PCR as Platform for its microRNA-based Diagnostics to be Launched in 2008, Licensed Nanogen's MGB Probe Technology

First microRNA-based Diagnostic Tests are Expected to be Launched in 2008 and Will Incorporate Nanogen's MGB Probe Technology Rosetta Genomics Will use the Licensed Technology in its Custom Designed Real-Time PCR Diagnostic Assays That Allow for Sensitive Detection and Quant...

QIAGEN Fast Cycling PCR Kit

For fast and specific PCR on any thermal cycler The QIAGEN Fast Cycling PCR Kit provides the unrivaled performance of HotStarTaq Plus DNA Polymerase with a significantly shortened PCR cycling time on any thermal cycler. The...

QIAGEN LongRange PCR and 2Step RT-PCR Kits

For reliable and accurate long-range PCR and RT-PCR QIAGEN LongRange PCR and LongRange 2Step RT-PCR Kits provide dedicated and highly reliable solutions for long-range amplification from genomic DNA and cDNA. Low error rates are assured using a novel high-fidelity e...

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

Introduction Amplification of DNA through the polymerase chain reaction (PCR) is a highly sensitive method capable of copying millions of DNA molecules from just a single molecule. 1 Because of the extreme sensitivity of this method, ...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

Jessica N Ebright 1 and Catherine Bowes Rickman 1, 2, 1 Departments of Ophthalmology and 2 Cell Biology, Duke University Medical Center, Durham, NC 27710 USA Correspondence: Catherine Bowes Rickman, PhD, Department of Ophthalmology, Duke Univ...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

Giles Cunnick and Wen Guo Jiang, Metastasis Research Group, University Department of Surgery, University of Wales College of Medicine, Cardiff, UK Introduction Lymphangiogenesis refers to the formation of new lymphatic vessels. This process...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

Faye Boeckman, Larissa Tan, Marni Brisson, Rob Park, and Keith Hamby, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 Introduction When researchers need to measure the amount of RNA or DNA in a preparation, they t...

Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix

Paul Burr, Wellcome Clinical Scholar, Department of Veterinary Pathology, University of Glasgow, Bearsden Rd, Glasgow. G61 1QH. Introduction A method that can be conveniently used to prepare DNA from a wide range of tissues for analysi...

InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C

Prepare DNA from blood, cultured cells, or bacteria: Just incubate sample with InstaGene matrix at 56C for 1530 min, then boil for 8 min. InstaGene matrix absorbs cell lysis products, producing an improved substrate for PCR amplif...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

Surekha Karudapuram, Ph.D. and David Batey, Ph.D. MJ Research, Inc., South San Francisco, CA Abstract We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to b...

Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A

Jolanta Kolodziejek, MSc, and Professor Norbert Nowotny, PhD, Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, Vienna , Veterinaerplatz 1, A 1210 Vienna, Austria Introduction The purity of t...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

Menu B. Leddy, Biotechnology Research Department, Orange County Water District, 10500 Ellis Ave., Fountain Valley, CA 92708 Introduction Identification and characterization of bacterial species from membrane biofilms has been a microbio...

GENE SILENCING WITH PCR PRODUCTS

siRNA Expression Cassettes siRNA expression vectors offer enormous opportunities to study gene silencing. Unfortunately, the preparation of siRNA vectors is labor and time intensive. Ambio...

Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method

Occurrence of tumor cells in the peripheral blood of individuals suffering from cancer may serve as an early indication that the primary tumor has dispersed from its tissue of origin. Despite defence mechanisms of t...

Screening of Beer-Spoilage Bacteria Using the LightCycler PCR Workflow System

The new LightCycler foodproof Beer Screening Kit (dedicated to the detection of beer-spoilage bacteria on the LightCycler Instrument) in combination with the MagNA Pure LC DNA III Kit (for the automated isolation of bacte...

Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System

Roche Applied Science pioneered the blending of thermostable DNA polymerases and the associated buffer systems. The well-known Expand High Fidelity and Expand Long Template PCR Systems were the first commercially availa...

Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

The endopeptidase originally isolated from the filtrate of the lower fungus Tritirachium album limber is recombinantly expressed in Pichia pastoris. Proteinase K is extremely effective on native proteins from different so...

Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System

The FastStart Taq DNA Polymerase FastStart Taq DNA Polymerase, a chemically modified version of Taq DNA Polymerase for hot start PCR, was introduced in 2000. Based on the feedback f...

Rapid PCR* Using Active Tube Temperature Control On Thermo's PCR Machines

Thermo Electron Corporation Solutions Note The time taken to complete a standard PCR protocol is becoming increasingly important for researchers today. Whilst many manufacturers of thermal cyclers focus on pushing the limits of ramp rates, in this article we h...

Properties of PCR Enzymes

The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75C and the ability to retain that activity after prolonged incubation at even higher temperatures (95C). Initially, single DN...

High Pure PCR Template Preparation Kit

For isolating genomic nucleic acids for PCR, restriction enzyme analysis, and Southern blotting Catalog Number Pack Size ...

High Fidelity PCR Master

for amplification of up to 5 kb targets from genomic DNA Cat. No. 2 140 314 200 reactions Description Premixed, 2x concentrated High Fidelity PCR Master contains all necessary reagents (buffers,...

Expand Long Template PCR System

for amplification of 520 kb targets from eukaryotic genomes Cat. No. 1 681 834 100 units Cat. No. 1 681 842 2 x 250 units Cat. No. 1 759 060 10 x 250 units Description Unique enzym...

Expand 20 kbPLUS PCR System

for amplification of long targets (up to 35 kb) from eukaryotic genomes Cat. No. 1 811 002 100 units Description Unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzy...

Find the optimal product for your PCR application

Which of the following statements best describes your template/starting material: ...

Expand High Fidelity PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets Cat. No. 1 732 641 100 units Cat. No. 1 732 650 500 units Cat. No. 1 759 078 2500 units Description Unique enzyme...

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are! Choose the LightCycler PCR System, the only instrument flexible enough to meet your changing research needs. Central to the LightCycler ...

GC-RICH PCR System

for amplification of GC-rich targets (up to 5 kb) from genomic DNA Cat. No. 2 140 306 50 PCR reactions Description Unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostab...

Expand High FidelityPLUS PCR System

Explore New Horizons in Amplification Yield Combine High Yield, Accuracy, and the Prevention of Carry-Over Contamination Roche Applied Science, which pioneered the blending of thermost...

QuantiTect Probe PCR Kit

For quantitative, real-time PCR and two-step RT-PCR using sequence-specific probes Component Included Concentration ...

QIAquick PCR Purification Kit

For cleanup of DNA from PCR or other enzymatic reactions Application: PCR cleanup Format: Spin columns ...

QIAGEN PCR CloningPlus Kit

Includes QIAGEN EZ Competent Cells for added convenience Component Included Concentration ...

MinElute PCR Purification Kit

For purification of PCR products in low elution volumes Application: PCR product cleanup Format: Spin columns ...

Successful PCR amplification and subcloning of a GC-rich DNA fragment

Petra Majlingov, Paolo Paroncini, Silvia Svegliati, Michele Luchetti, and Armando Gabrielli Laboratorio di Medicina Molecolare, Istituto di Clinica Medica, Polo Didattico Scientifico, Ancona, Italy The formati...