nitro

Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq

University of Georgia, Department of Marine Sciences This application describes the use of the MasterTaq polymerase kit for improving PCR efficiency when studying functio...

Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

adenoviral vector ● viral transduction ● NTR ● IN Cell Analyzer ● gene delivery Biological assays developed in cultured and primary cells can greatly aid secondary screening as well as lead and target validation, but their development presents several challenges. Establishing stable cell systems to expre...

Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

This application note illustrates an improved method to quantitate Nnitrosamines in baby bottle teats using APCI-MS. Six rubber nipple formulations were analyzed. Two formulations were composed of natural rubber, two were composed of a synthetic silicone polymer and the remaining two were composed of a synthetic polypropylene. The natural rubber formulations were found to have a signi...

Detection of nitrofurane metabolites in food with HPLC/MS/MS

Furazolidone, Furaltadone, Nitrofurazone and Nitrofurantoine belong to the group of nitrofuranes. As antibiotics they were used for treatment of gastrointestinal infections in cattle and poultry. Due to its proven mutagenic and carcinigenic effect, nitrofuranes (except for furazolidone) was prohibited in 1993 in the European Union (VO (EWG) No. 2901/93). Furazolidone was prohibited in...

High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS

The animal meat samples were prepared by homogenization, organic solvent washes, hydrolysis and derivatization follo...

Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS

Trace amounts of nitr...

Quantitation of a Dinitro Flavonoid (DNF) in Rat Plasma Using the Varian 1200L LC/MS/MS System

Schmidt, Varian, Inc., Walnut Creek, CA Flavonoids are compounds that make up the pigments in fruits, vegetables, and herbs. There have been reports of flavonoids having multiple beneficial therapeutic effects for a large number...