mine

Imaging Chemiluminescent Western Blots

II still video system to directly acquireimages from chemiluminescent blots Stratagene II still video system is well suited for directimaging of chemiluminescent Western blots. The light-tight cabinet is ideal forthe longer exposure times necessary to...

Using Gradient PCR to determine the optimum annealing temperature.

Problems with the amplification of a specific DNA fragment using the PCR* are an everyday occurrence in the lab. For example, unspecific secondary bands may form after the PCR reaction, which hinder, or even prevent, further analyses (cycle sequencing, mutation detection, etc.) or an unequivocal assessment of the PCR r...

MinElute PCR Purification Kit

For purification of PCR products in low elution volumes PCR product cleanup Spin columns 124 samples...

A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option

In high sensitivity flash type luminescence assays the measurement is done just after dispensing the activating reagent or after a short delay time. Over the past years luminescence substrates have been improved towards providing more stable signal. These so-called glow type substrates allow microplates to be counted without dispensors. Ho...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently count...

Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)

Jinfang Liao, M.D., Ph.D. and Evelyn McGown, Ph.D. Molecular Devices Corporation, 8/00 The photoprotein aequorin, a bioluminescent protein from the coelentratejellyfish Aequorea victorea, has proven to be highly sensitive to the trace amount of Ca in cells. The aequorin complex which contains the...

UW study examines public attitudes on nanotechnology

"The message to scientists is: The public is not going to come to you. You have to take your work to the public, and make it availa...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...

Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System

AMARA • kinase assay • LEADseeker • chemiluminescence It is predicted that at least 500 kinase and phosphatase enzymes exist, their activities having wide-ranging regulatory responses. Many kinases are part of a signaling cascade in disease pathways such as inflammation and cancer, so identifying modulators of kinase...

Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System

receptor • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads Histamine is a biogenic amine that plays important pathophysiological roles in central and peripheral tissues. Generally released from stimulated mast cells, it contributes to several biological processes that characteri...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

<P >J. Turner GE Healthcare, The Maynard Centre, Cardiff, UK Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ Kinase Assay Kits gene...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence assays. LEADseeker™ Multimodality Imaging System was de...

Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System

Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence cell-based ass...

Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

This application note illustrates an improved method to quantitate Nnitrosamines in baby bottle teats using APCI-MS. Six rubber nipple formulations were analyzed. Two formulations were composed of natural rubber, two were composed of a synthetic silicone polymer and the remaining two were composed of a synthetic polypropylene. The natural rubber formulations were found to have a signi...

Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS

Amphetamine drugs are often abused and misused in sports, in the work place, and by recreational users. Forensic, clinical, and doping laboratories are frequently asked to analyze for the presence of amphetamines in urine. Urine samples are most common because large sample volumes can be...

Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

P by Cerenkov counting, a well-known measurement technique that has the...

Photoluminescence Spectroscopy of Quantum Dots, monitored by the Spex FluoroMax

The optical properties of quantum dots (QDs) have potential applications in optoelectronics, biosensing and biolabeling, memory devices, and sources of laser light. For example, alloyed CdSeTe QDs are shown herein to possess a nonlinear change in their photoluminescence spectra, correlated to size and composition, as monitor...

Time gated separation of lanthanide luminescence

The distinctive luminescence of lanthanide ions has long been a challenge for the spectroscopist. Methods to counter the extremely low absorption coefficients and luminescence quantum yields have concentrated on Protective ligands to reduce vibrational quenching effects by XH moieties (X = O, N,)....

Glutamine in Urine or Blood Plasma

UV/Visible HPLC detector Typical Method: F <str...

Prominence UFLC

Prominence UFLC has been designed to achieveUltra-Fast HPLC by utilizing the Prominencehardware series. In this respect, we are successfulin demonstrating high speed and separation performancewithout resorting to high pressure, while providing highreliability and expandab...

IT Task Force to examine Workforce Development projects

The hearing, which will focus on three Department of...

Pre-eminent, web-based companies owe success to corporate venturing

But the Milwaukee-based company might never have attained that stature or gotten off the gro...

Make Mine a $Million shoots for May program

Although the date of the awards program has not yet been made official, Wisconsin's female entrepreneurs - incl...

WARF stem cell patents to be re-examined

The challenges, brought by the <a href="http...

Request to re-examine WARF stem cell patents escalates war of words

The most recent exchange came after the , on be...

U.S. health system could undermine EHR progress

Dr. Jeff Grossman, president and CEO of the Univers...

Before deciding how to pay for K-12 schools, let's determine what to buy

- It's not surprising that Gov. Jim Doyle and the Republican-led Legislature are still at odds over how the state's latest budget will treat public school districts across Wisconsin. Doyle thinks he saved K-12 districts from cuts without hurting taxpayers; the Republicans believe Doyle abused his veto power and put more spending on the credit ca...

UW-Milwaukee Chancellor Santiago determined to build on research base

Now that he's taken up residence in Wisconsin, Santiago sees no reason why UW-Milwaukee can't aspire to do the same. Santia...

Symposium examines the effect of race on genetics and disease

MADISON Health statistics are often broken down into categories by race. But increasingly, scientists question whether race is a meaningless concept that has no basis in biology or genetics. This was the concern of 40 scientists and ethicists at a symposium titled Race, Genetics & Disease at Grainger Hall April 16 and 17. The University of Wisconsin-Madisons <a href="http://www.sts.wisc...