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A New PCR-based Mycoplasma Detection Method

Stratagene akit that uses a PCR-based method which specifically amplifies an 874-bpPCR product from a single copy gene. In addition, the kit contains an int...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

Mark Churchill and Mark Harrison, Thermo Finnigan, Hemel Hempstead, UK The data presented here can be acquired using a Thermo Finnigan TSQ Quantum mass spectrometer...

Comparison of Different Methods of mRNA Quantitation

<TABLE width="96%" border=0 align="left" cellPadding=8 cellSpacing=1> Nuclease Protection Assays <b...

Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

Fang-Fang Wu, Xuemei He, and David R Nau, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA Immobilized metal affinity chromatography (IMAC) is a powerful technique that can be used for the efficient purification of recombinant histidine-tagged (His-tagged) proteins from a variety of ex...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. 1</...

Scientists find nanotech method for examining cells

The crystals, sometimes known as quantum dots, are tiny on the order of a handful of molecules. Researchers, including University of Wisconsin-Madison professors Dan van der...

Comparison of different protein quantitation methods

This application note compares the accuracy and reproducibility ofabsolute protein quantitation of the Agilent 2100 bioanalyzer in combinationwith the Protein 200 Plus assay to the batch-based methodsLowry and Bradford as well as to denaturing gel-elctrophoresis (SDSPAGE)....

Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA

This Application Note describes how Gene Logic, Inc., has improved itsability to analyze the quality of total RNA and fragmented cRNA by usingthe Agilent 2100 bioanalyzer. The Eukaryote Total RNA Nano and Picoassays are currently being utilized to examine the 28S/18S ratio...

Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method

Occurrence of tumor cells in theperipheral blood of individuals sufferingfrom cancer may serve as anearly indication that the primarytumor has dispersed from its tissueof origin. Despite defence mechanismsof the organism, individualcancer cells may attach in di...

DNA-free A NEW Method to Remove DNA

Now it's easy to make your RNA free of genomic DNA contamination and ready for RT-PCR. DNA- DNase Treatment & Removal Reagents contain RNase-free DNase, and an optimized DNase digestion buffer, to ensure safe, complete removal of contaminating DNA from any RNA sample. Also included is a unique...

RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control

RNase activity varies dramatically across different tissues. A comparison of total RNase activities for 8 different mouse tissues showed that total RNase activity spans a 181,000-fold range from pancreas to brain (Figure 1), which highlights the level of RNase control required for succ...

Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A

Jolanta Kolodziejek, MSc, and Professor Norbert Nowotny, PhD, Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, Vienna , Veterinaerplatz 1, A 1210 Vienna, Austria The purity of the DNA template employed in sequencing PCR (cycle sequencing) is the most...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

Kathleen A. Maguire-Zeiss, Brandon K. Harv e y, Rita E. Giuliano andH o w a rd J. Federo ff, University of Rochester, Rochester, NY, USA Transfection efficiency is dependent upon a number of factors, including method of transfection, cell type, and DNA concentration and purity. Liposome-mediated transfect...

Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

and Aiguo Zhang, 1 Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, and Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, TheUniversity of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. BiPs...

A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders

The peroxisome is a cellular organelle that plays an important role in a number of functions, including the beta-oxidation of very long chain fatty acids (VLCFA). Peroxisomal disorders are characterized by impaired, reduced or total absence of peroxisomes in cells, which can result in the accumulation of VLCFAs, such as tetracosanoic and hexaco...

Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method

They are not as commonly available for novel sample matrices such as saliva as...

A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase

In traditional DNA sequencing systems, sequencing products are radioactively labeled using either endlabeled primers or by the incorporation of radiolabeled dNTPs during primer extension. Successful sequencing results produced by these two labeling methods are highly dependent on template quality. DNA containing nicks, breaks, impurities o...

A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors)

In clinical research, the monitoring of protease inhibitor (PI) and non-nucleoside reverse transcriptase inhibitor (NNRTI) antiretroviral drugs is of significant interest due to the importance of assessing inter-individual and intra-individual metabolism variability. To be of practical use, it is essential that the analytical method used to mon...

A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

A previously published application note in this series describes an LC/MS/MS method for the simultaneous determination of four immunosuppressant drugs: Cyclosporin A (CsA), Tacrolimus (TAC, aka FK-506), Sirolimus (SIR, aka Rapamycin) and Everolimus (EVE, aka RAD-001) (1, 2). The method is based on a simple and robust hardware configuration and...

Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS

Testing for drugs of abuse (DoA) is becoming more prevalent in various environments, including forensics and social justice environments as well as in sporting events. Non-heroin opiates such as morphine, oxycodone, and hydrocodone are appearing increasingly in drug indicator data. For examp...

Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method

. CYPs are part of the Phase I (oxidative) metabolism within the body, which introduces a hydrophilic site on the metabolized drug enabling either easy elimination...

Analysis of Fuel Oxygenates by U.S. EPA Method 8260B Using Headspace Trap with GC/MS

710 Bridgeport Avenue Shelton, CT 06484 The Clean Air Act amendments of 1990 require that oxygenated compounds be added to gasoline to produce fuel with increased oxygen content. Oxygenat...

Endotoxins and their Relevance in R&D ApplicationsLPS Detection & Removal Methods

Contaminations of cell cultures show severe problems in the research and production industry. The attention turns here particularly to the prevention of any contamination with bacteria, Mycoplasmas, yeast or fungi. For companies that develop substances for therapeutic applications endotoxins are nowadays a main focus....

FDA: Tortoise, hare, or something else?

In...

With tech implementations, it's NOT the methodology, stupid

Each company prese...

Mirus announces new method for making antibodies

Antibodies, naturally part of the immune system, are manufactured both for the treatment of diseases and for diagnostics or research. Mirus' new technique, described in the February issue of BioTechniques, involves intrave...

Technology puts pressure on old education methods

Judy Brown, an analyst of emerging technology for the University of Wisconsin-Madison and director of the Madison chapter of the World Future Society...

This FlashMob is something super

What are you doing on April 3? If you're in the San Francisco area, you could be part of something big. Really big. Super big. On that one day, the University of San Francisco will attempt to create an ad hoc, networked supercomputer from the assembled processing power of an expected 1,000 laptop-toting geeks. The goal of the event, called "FlashMob Computing Supercomputer: FlashMob I",...

Wisconsin Engineers Devise Cutting-Edge Method to Cut Cheese

Madison, Wis. - Lasers do everything these days - from removing tattoos to playing music on compact discs. Now, in the great dairy state of Wisconsin, lasers have been harnessed to an entirely new purpose: slicing cheese. Xiaochun Li, a University of Wisconsin-Madison mechanical engineering professor and laser expert, along with graduate student Hongseok Choi, has become the first to ada...