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measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader

Evelyn McGown, Ph.D., Jinfang Liao, M.D., Ph.D., Mary Kassinos, Irina Osetinsky and Jayne Hesley, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. They can be cloned in a diverse range of cells and org...

Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers

Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas prot...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

1 GFP (Green Fluorescent Protein) is an extremely stable protein of 238 amino acids produced by the jellyfish that fluoresces in the lower green region of the visible spectrum....

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

October 1999 Molecular beacons are fluorogenic oligonucleotide probes developed by Kramerand associates to detect specific nucleic acids in homogeneous solutions. They are single-stranded oligonucleotides that exist in a hairpin shape because of thecomplementary arms at the ends which bind together to form a stem....

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also...

UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)

Molecular Devices Corporation. 7/99 In 1994, Molecular Devices introduced SPECTRAmax , the first microplate reader equipped with a monochromator, rather than bandpass filters, for wavelength selection. It was also the first microplate reader capable of measuring absorbance below 340 nm. These new features transformed the micropl...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...

Cell Proliferation and Viability Measurement

The quantification of cellular growth, including proliferationand viability, has become an essential tool in anylaboratory working on cell-based studies. Such techniquesenable not only the optimization of cell cultureconditions, but also the determination of growth factorand...

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

This application note describes how the Agilent 2100 bioanalyzer canbe used in combination with Standardized RT (StaRT)-PCR reagentsfrom Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville,AL, USA) to obtain highly reproducible, standardized gene expressionme...

The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays

Apoptosis represents a gene-directed, morphologically and biochemically distinct form of programmed cell death. Inappropriate control of apoptosis is associated with a variety of disease states such as cancer, AIDS, neurode...

Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells

SPA Imaging Beads • LEADSeeker • cAMP • receptor binding assay SPA Imaging Assays are homogeneous immunoassays based on the coupling of one assay component to an SPA Imaging Bead in order to generate a signal. Polystyrene (PS) SPA Imaging Beads coated with donkey anti-rabbit antibody enable the immobilization of...

LabVIEW for Measurement and Data Analysis

housands of engineers and scientists rely on LabVIEW for a variety of applications: test and measurement, process control and automation, monitoring and simulation. LabVIEW is the tool of choice due to its unparalleled connectivity to instruments, powerful data acquisition capabilities, natural dataflow-based graphical programming interface, scalability, and overall function comp...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

SmithKline Beecham Pharmaceuticals, UK FlashPlate is a 96-well white polystyrene microplate with scintillant-coated wells designed for highvolume, in-plate radiobinding assays. This study investigates the use of FlashPlate as a platform for the measurement of G-protein act...

Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors

SmithKline Beecham Pharmaceuticals, UK Due to recent technologies in the pharmaceutical industry that have resulted in an increase in the production of potential drug candidates, it has become vital that screening assays are high throughput. FlashPlate technology from PerkinElmer Life Sciences has pro...

Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor

Dpartement dImmunologie, Laboratoires Fournier S.A., France TGF- type I and type II receptors form ligand dependent heteromeric signaling complexes, in which transforming growth factor- receptor type II (TRII) tends to act as the primary receptor....

In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

Abnormalities in neurotransmission within the brain can lead to several disease states, including anxiety, depression or schizophrenia. Extensive research has been carried out to discover the mode of action of various transmitters, in order to understand and combat these diseases. In many cells, binding of the neurotransmitter to its recept...

Singlet Oxygen measurement

The chemistry, spectroscopy, and reactivity of singlet oxygen, , su...

Phosphorescence lifetime measured using the SPEX phosphorimeter

A phosphorimeter integral to the SPEX system can uncover important information on various systems of chemical and biochemical interest, by revealing data normally masked by intense but rapid fluorescence. <P>This Application Not...

Joint Finance blocks Doyle ethanol measure

for rejecting a proposed ethanol mandate earlier this year. Now, the <a href="http://www.legis.state.wi.us/lfb/jfc.html"...

Broadband deployment measure goes to governor

The bills, Assembly Bill 892 and Senate Bill 483, are known as the Broadband Deployment Act. They would provide $7.5 million in income tax credits to pr...

Windows exploit worsens as stop-gap measures fill in for official patch

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Wisconsin Senate votes to outlaw cloning; measure still faces veto

The bill was supported by all Republicans and two Democrats: Sen. Jeff Plale, D-South Milwaukee, and Sen. Roger Breske, D-Eland. The bill, AB 499, would outlaw not only cloning for...