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lid in Biological Technology

Sirion Therapeutics Partners With Advanced Vision Research (TheraTears(R)) to Promote Products for Retinal and Meibomian Gland Health

...se of evaporative dry eye and is associated with ocular rosacea. Meibomitis is an incapacitating condition characterized by excess bacteria in the eye lid margins with increased vascularization of the lids, and loss of normal meibomian gland anatomy and function. This leads to diminished quality and dim...

Corporations and blogging

... of the legal action has spread around the blogosphere (a search of "Apple sues blogger" on Feedster delivered 197 results). For its attempts to put a lid on leaks, Apple comes out looking like a big bully that picks on a kid with a blog because it can't control its own employees. Certainly that's not th...

DHC 2007: Barriers to electronic data exchange include the law

...hange open a Pandora's box of liability, or help health providers close the lid on a box of horrors that already exists? When an error is made, it's go...t comes to assessing whether electronic patient data exchange will blow the lid off Pandora's box, or provide an airtight seal, DeLair believes the answer ...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

... method: Pipet 150 L aliquots of each sample, standard and blank into the designated wells of the microplate. Add 150 L reagent to each well. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. Incubate the plate at 37 C for approximately one ...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

...9674; Incubation oven ◊ Microcentrifuge ◊ Arcturus alignment tray ◊ Thermo Cycler with heated lid ◊ Horizontal gel chamber ◊ Power supply ◊ Gel Doc 2000 TM Documentation System (Bio-Rad, Cat. # ...

RNA Amplification Just Got Better: MessageAmp II

... strand cDNA synthesis, DNA polymerase was added for second strand synthesis, which was incubated at 16C for 2 hr in a thermocycler (heated lid option turned off). The double-stranded cDNA was then filter purified and added to a 4 hr in vitro transcription reaction. Last,...

ArrayScript RT: Maximize Yields of Full-length cDNA

... cDNA synthesis, DNA polymerase was added for second-strand synthesis, which was incubated at 16C for 2 hr in a thermocycler (heated lid option turned off). The double-stranded cDNA was then filter purified and added to a 4 hr in vitro transcription reaction. Last,...

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

...nespecific primers and cycled either in a Rapidcycler (Idaho Technology, Inc., Idaho Falls, ID) or PTC- 100 block thermal cycler with heated lid (MJ Research, Inc., Incline Village, NV) for 35 cycles. In each protocol the denaturation temperature was 94 C, the annealing temperature was ...

S.E.T.S. Swab Extraction Tube System

...y 5 8 mm in diameter and 1025 mm in total length) in the Inner tube (1). The Inner tube has a hole with a defined diameter on the bottom. The lid is then closed, and the loaded Inner tube is placed into the Collection tube (2). The assembled device is now placed in a standard benchto...

HotStarTaq PCR Handbook

... Enzyme: (See quality-control label inside kit lid for lot-specific values.) ...overlay the PCR samples with mineral oil if the heated lid is switched on as this may decrease the yield of PCR p...

QIAGEN Multiplex PCR Handbook

...When performing PCR in a thermal cycler with a heated lid, do not overlay the PCR samples with mineral oil if the heated lid is switched on. This may decrease the yield of PCR product. 14. Problems with the the...

Mastercycler Gradient Quick Programming Guide

... Program temperature of heated lid and switch on or off, Use key to turn on lid Temperature displayed as Nominal/ Actual Temperature, heated lid switched off when a temperature ...

Title

... LID=105 Heated lid set to 105 C. ... = Cycling does not start until set lid temperature is reched. ... AUTO = lid heating is switched off automatical...

Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

... Block temperature control Heated lid temperature Program starts immediately lid shuts off at end of program ...

7 Programming

... CNTRL TUBE lid NOWAIT FIX The entries in the first program are also valid for the programs which are added via LINK. The lid setting "Fix" must be selected. ...

Brinkmann/Eppendorf SOP Standard Operating Procedure for Pipettes

... which come in direct or indirect contact with the pipettes must be prevented. The tubes provided by Eppendorf and the moisture trap with lid are made of glass, which minimizes electrostatic charge. Measuring station: Calibration software and accessori...

Chemical Stability of Disposables

... - When lid open ... - When lid open ...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

...he master mix could be added to 15 l of the DNA sample for a total reaction volume of 25 l. The master mix was pipetted into the lid of the corresponding reaction tube. The lids were closed, and the tubes were briefly centrifuged to add the master mix to the DNA ...

A membrane-lid tube for bacteria cultivation and freeze-drying

...ut. The membrane-lid tube lid Bac from Eppendorf is a significant co... the suitability of the membrane-lid tube lid Bac for bacteria cultivation on a minipr...etween the growth behavior of bacteria in lid Bac and in conventional Erlenmeyer flasks. ...

High-Throughput Cultivation of Bacterial Clones

...of the plate were removed by perforating the plate lid with a hot needle just prior to sampling. This method prevented the need to remove the lid from the plate and introduce air into the nonaer... in two, 96-deep-well plates. A special lid seals each well and prevents cross cont...

Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results

...ng using both a RoboCycler 96 temperature cycler fitted with Stratagenes Hot Top Assembly and an MJ Research PTC-100 with heated lid enabled. In each case, fluid temperature measurements were taken in six tubes positioned in the four corner wells and two center...
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