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New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

Stratagene hybrid genes are used to detect protein-protein interactions via the activationof reporter gene expression. This expression occurs as a resul...

New Temperature Cycler Features Interchangeable Blocks

Michelle Cayouette Jane Moores Jason Capodanno Michael Steege Michael Rahilly Stratagene Infinitytemperature cycler, a new temperature cycler withinterchangeable blocks for...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

Stratagene A 28-base insertion was introduced into a plasmid vector using theQuikChange site-directed mutagenesis kit.* Theinsertion created three unique restriction enzyme digestion sites. Plasmid DNAisolated from 9 o...

Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells

Department of Cell Biology, Neurobiology, and Anatomy University of Cincinnati College of Medicine, Cincinnati, OH 45267 Transfection of mammalian cell lines is enhanced by the use ofliposome-based reagents. Here we compare the transfection e...

Enzyme Immunoassay for Studying Intracellular Levels of cAMP

Sensitive, nonradioactive cyclic-AMP detection kit and adenylate cyclase(AC) toxin Stratagene Cloning Systems, Inc. American Qualex Stratagenes EIA cAMP Kit is a highly sensitive and specificantibody-based assay kit for no...

TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

Institute for Molecular Biology and Biotechnology, McMaster University,Ontario, Canada GST-fusion proteins expressed in TKB1 bacteria are used to affinity purifycellular proteins, which interact with a specific autophosphorylation site onthe Erb...

Mammalian Expression Vectors with Interchangeable Selectable Markers

Site-specific recombinase-based vector system Stratagene system of eukaryotic expression vectors offers unparalleled convenience. The drug-resistance genes of these vectors can be exchanged without subcloning. Through site-specific recombination, hygromycin, puromycin,...

New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

Stratagene cis-reportingsystems now includes the GAS and ISRE reporting systems, to better study theactivation of signal transduction pathways stimulated by int...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

Stratagene cis-reporter plasmid,the pNFAT-Luc reporter plasmid, is designed for studying the activation of thenuclear factor of activated T-cell (NFAT) transcription factors...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

reporting system for studyingNFAT activation Stratagene cis-reporterplasmid, the pNFAT-Luc reporter plasmid, is designed for studying the activationof the nuclear factor of activated T-cell (NFAT) tr...

Printable CELLocate Log Sheets for Easily Locating Adhererent Cells

Log Sheets for Easily Locating Adhererent Cells CELLocate is the ideal solution for relocating adherent cells that have been used for microinjection. These sterile coverslips come with a microgrid pattern. Using the CELLocate proto...

Intracytoplasmic Spermatocyte Injection (ICSI)

The number of infertile couples has increased in recent years and is now estimated at 1520% of all couples worldwide. Since its introduction into reproductive medicine in 1978 , in vitro fertilization has been considered a successful method of treating tubal ste...

Mycobacterium intracellulare

Mycobacterium intracellular...

Mycobacterium intracellulare

Mycobacterium intracellular...

Analyzing Trace-Level Impurities of a Pharmaceutical Intermediate Using an LCQ Deca Ion Trap Mass Spectrometer and the Mass Frontier Software Package

Mark R. Kagan , Carrie Liu 1Thermo Finnigan, Piscataway, NJ; 2Thermo Finnigan, San Jose, CA; 3Eisai Research Institute, Andover, MA The data presented here can be a...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

Mark Churchill and Mark Harrison, Thermo Finnigan, Hemel Hempstead, UK The data presented here can be acquired using a Thermo Finnigan TSQ Quantum mass spectrometer...

RNA Interference and Gene Silencing: History and Overview

May 20, 2002 Post-transcriptional gene silencing (PTGS), which was initially considered a bizarre phenomenon limited to petunias and a few other plant species, is now one of the hottest topics in molecular biology (<A href="#1">1 ). In the last few years, it has become clear that PTGS occurs in both plants...

RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater

is a tissue storage solution that stabilizes and protects cellular RNA in fresh tissues. (Ambion's new RNA -ICE is designed specifically for use with frozen tissues - see "<A href="http://www.ambion.com/techlib/tn/103/1.html">Stop Grinding...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

Martin A.O.H. Menke*, Axel Reinecke-Lthge*, Barbara Mllmann*, Angela Kellner, Jutta Lttges*, Gnter Klppel* * Department of Pathology and Department of Hematopathology, Christian- Albrechts University Kiel, Michaelisstrae 11, D-24105 Kiel, Germany. Introduction Genetic alterations/variations are...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently count...

Improving an Integral Part of the Lab: Vacuum

PowerDry vacuum pumps; a solution for applications producing condensation Where gases with high moisture content must be evacuated during vacuum drying operations, condensate may collect in the vacuum pump head. Consequently, the pump may not perform properly and may take more time to evacuate the designated volume. New, PowerDry...

Quintessence Biosciences names new president

The appointment was made by the company's board of directors and announced at the company's annual shareholder's meeting. <...

Viewing the Internet as a free-for-all can be costly

According to a recent study, nearly 94 percent of all electronic mail on the Internet is unsolicited <a href="http://spam.abuse.ne...

Funds will support public safety communications interoperabilty

The funds will be used to implement a regional public safety mobile radio plan for the 16-county group, and purchase equipment that will ena...

Alfalight introduces new 25-watt pump module

The CPM-II is available with 25 watts of output power and a 200-micron (0.22 NA) output fiber for fiber laser applications. A version with 21 watts in a 6+1:1 pump/signal con...

Debate over `intelligent design' is a not-so-wise diversion from science education

- The day an archeologist finds a fossil cat or dog in Precambrian rock is the day Charles Darwin's evolution theory is disproved. But that hasn't happened. In fact, the Earth's fossil records tell a consistent scientific story through the ages: Today's living species descended -- with modification -- from common ancestors that lived in our anci...

High interest forces delay in food safety symposium

A surge in interest in a conference on protecting the U.S. food supply from terrorism and natural disasters has led its organizers to postpone the event until spring from its original November dates. The was originally slated for Nov. 1-3 in downtown Milwaukee, with as many as 1,000 people expected from the Gr...

Madison start-up bets on interactive future for reading education

The company...

Biotech industry reaches midpoint in 50-year maturation cycle

An interesting article in ) on March 1 states unequivocally that most industry cycles run about 50 years until they mature. If this...

Icon Interactive creates new Web monitoring tool

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MobilePro to aquire Janesville-based internet service provider

Janesville, Wis. , a Janesville-based Internet services provider. The deal is forecast to add more than 15,000 subscribers, $2 million in annualized revenue and to be accretive to MobilePros ear...

Nebraska bank benefits from Fiservs integrated solutions

Brookfield, Wis. A Midwestern community bank can now remove the guesswork out of customer profitability analyses, based on the integration of two technologies from two separate divisions of Wisconsin-based Fiserv. , located in Laurel, Nebraska, can now view financial relationship data from any of its desktops and ident...

UW Medical Improves Business Intelligence

MADISON, WI- The has chosen to standardize on a business intelligence t...

Exclusive Interview With Dick Leazer: Renaissance Man

Madison, WI, Jan. 21 - From 1993 through 1999, Dick Leazer served as managing director of the Wisconsin Alumni Research Foundation (WARF), the licensing and commercialization agency of the University of Wisconsin. Since then, he has co-managed the Wisconsin Investment Partners (WIP), an angel investment group based in Madison that focuses on funding life sciences start-ups. Through these two orga...

Covance Appoints Co-Presidents for Early Development Services

PRINCETON, NJ - Covance Inc. has promoted Wendel Barr to the position of president, early development, in Madison, Wis., and Anthony Cork to the position of president, early development, in Harrogate, UK. The company said the move supports the regional decision-making employed by its clients in the preclinical segment. With the promotion, Wendel and Tony have become corporate senior vic...

SPRINTPREP SINGLE STEP PLASMID PURIFICATION

SprintPrep is an efficient SPRI-based plasmid purification system that can be completed in less than 15 minutes. Unlike most traditional plasmid purification chemistries, SprintPrep isolates plasmid DNA directly from overnight cultures, which can improve the turnaround time of a sequencing pipeline by as much as a full...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to detect cell surfaceand intracellular protein targets by antibody staining. CD3 staining onJurkat cells as well as CD4 staining on CCRF-CEM cells and intrace...

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

This application note describes how the Agilent 2100 bioanalyzer canbe used in combination with Standardized RT (StaRT)-PCR reagentsfrom Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville,AL, USA) to obtain highly reproducible, standardized gene expressionme...

Introducing GenElute HP Endotoxin-Free Plasmid Maxiprep Kit

Dave Cutter and Goodarz Goodarzi The GenElute HP Endotoxin-Free Plasmid MaxiprepKit offers a novel and efficient method of isolatingendotoxin-free plasmid DNA. A patent-pendingendotoxin removal process has resulted in the fastest,most streamlined protocol available...

Determinants of RNA Integrity and Purity

RNA quality is a critical determinant for the success of many downstream applications, including microarray analysis. For this reason, if you are planning microarray analysis experiments, it is important to fully consider the factors that affect the quality of your RNA. <P...