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gels in Biological Technology

Technical Note: E-PAGE(TM) 48- and 96-Well Gels on the Odyssey(R) Infrared Imaging System

LINCOLN, Neb., Nov. 19 /PRNewswire/ -- LI-COR Biosciences has released a new Technical Note for using E-PAGE 48- and 96-well gels the Odyssey Infrared Imaging System. The E-PAGE 48- and 96-well electrophoresis systems (Invitrogen Corporation, Carlsbad, CA) provide a high-throughput SDS-PAGE meth...

Bluestar Silicones Presents Adhesive Gels for Wound Care at Medical Grade Polymers Conference

EAST BRUNSWICK, N.J., Aug. 13 /PRNewswire/ -- At the upcoming Medical Grade Polymers Conference, Dr. Remi Thiria, Bluestar Silicones R&D Team Leader, will present "Silicone Elastomer Gels: New Soft Adhesives for Wound Care." The conference will take place in Philadelphia, September 16-17. Ac...

Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

Divya Sharma 1 and Carolyn Seggerson 2 , 1 Tech/Aid, 7700 Edgewater Drive, Oakland, CA 94621 USA, and 2 Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Introduction Running PCR samples on agarose...

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

Introduction The transfer efficiency of large (>100 kb) DNAs from agarose gels to membrane is poor unless the DNAs are nicked prior to transfer. Although this has traditionally been accomplished with HCl depurination, UV irradiation ...

CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

The first precast gels for automated sequencing and genetic analysis Stephen Roemer Mary Jensen Harry Osterman Mark Vanek LI-COR Biotechnology Division LI-COR and Stratagene release the first commercially available precast gels for automated DNA sequencing and g...

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

New optical filter for Eagle Eye II still video system Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye Michelle Cayouette Jane Moores Stratagene Cloning Systems, Inc. Stratagene currently provides an optical bandpass filter with all Eagle Eye ...

Four New CastAway Precast Gels

CastAway precast gels* eliminate the need to manually pour gels, saving up to 4 hours preparation time. Stratagenes quality guarantee means no more gels lost to bubbles or leaking.All CastAway gels are guaranteed to maintain stability for a minimum of 60 days from the date of manufacture,...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acid purification kits Jeff Braman Scott Basehore Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, which provide reliable isolation of high-purity miniprep plasmid,...

BioSyntech Reports Positive Results from Pivotal Trial for BST-CarGel(R) Cartilage Repair Device

...platform technology is a family of hydrogels called BST-Gel(R), some of which are liquid at low temperature and solid at human body temperature. These gels can be injected or applied to a specific local site and offer beneficial properties for the local repair of damaged tissue such as cartilage, bone and...

Phosphagenics Announces Completion of Transdermal Patch Prototypes

...d both reservoir and matrix patches that contain TPM and oxycodone and produce sustained delivery. A reservoir patch is characterized by incorporating gels within the membrane, whereas matrix patches contain drugs in a solid form. The matrix patch is ideally suited for opioids such as oxycodone where abus...

Serica Technologies Receives FDA 510(k) Clearance for SeriScaffold(TM) Technology for Soft Tissue Repair

...ovide support and relief to damaged tissues, in order to promote restored function. The company's ligament grafts for ACL repair, surgical meshes, and gels are comprised of the fiber protein of the B. mori silkworm, which has centuries of human use. In pre-clinical studies, Serica's silk-based products...

ImQuest Presentations at CROI Report Significant Advances in Therapeutic and Microbicide Product Development Programs

...applied agent to prevent the sexual transmission of HIV. Ms. Watson was also an invited participant in a themed discussion entitled "Novel Microbicide gels and Rings". ImQuest is currently working on the development of IQP-0528 as both a gel and an intravaginal ring product in combination with other highl...

Polymer Science Releases New Silicone Gel Adhesive Products for MD&M West

... a real difference in neonatal and geriatric care." In addition to PS-1154, PS-1155, and PS-1156, Polymer Science also offers customized silicone gels that can be coated as continuous or discontinuous configurations on a variety of substrates and backings. Polymer Science manufactures products in con...

BioSyntech Achieves Enrolment Goal for Pivotal Trial of BST-CarGel(R), its Cartilage Repair Device

...platform technology is a family of hydrogels called BST-Gel(R), some of which are liquid at low temperature and solid at human body temperature. These gels can be injected or applied to a specific local site and offer beneficial properties for the local repair of damaged tissue such as cartilage, bone and...

BioSyntech to conclude enrolment for pivotal BST-CarGel(R) trial in February 2009

...platform technology is a family of hydrogels called BST-Gel(R), some of which are liquid at low temperature and solid at human body temperature. These gels can be injected or applied to a specific local site and offer beneficial properties for the local repair of damaged tissue such as cartilage, bone and...

Penn researchers demonstrate a flexible, 1-step assembly of nanoscale structures

...res from an array of 1 μm diameter silicon pillars spaced 2 μm apart on a square lattice. When exposed to the organic solvent toluene, PDMS gels swell by as much as 130 percent. As the osmotic pressure builds, the circular pores in the PDMS deform and eventually snap shut to relieve the stress...

ULURU Inc. to Present at the 8th World Biomaterials Congress

...itles of these posters are "Formulation and Preclinical Evaluation of Hydrogel Nanoparticle-Hyaluronic Acid Dermal Fillers" and "Hydrogel Nanoparticle gels as Breast Implant Filler Materials." Commenting on the upcoming presentation Dr. John St. John stated "I am very pleased to be an invited speaker at...

72% of Women Are Dissatisfied With Their Current Treatment of Vaginal Infection, Survey Finds

...ewswire/ -- Despite the high incidence of women's health conditions that require intra-vaginal drug delivery and the abundance of intravaginal creams, gels and ovules, a recent survey found that an alarming majority of women find their current treatment options "unacceptable" or "intolerable". The survey,...

Atomic force microscopy reveals liquids adjust viscosity when confined, shaken

...er and silicone oil when they were confined, meaning they behaved more like gels or glass. The researchers also showed that the relaxation times depended on...kably similar to that widely observed in metastable complex fluids, such as gels or supercooled liquids, noted Riedo. Because we observed these phenomena in...

Kiwa Bio-Tech Products Group Discusses Cooperative Efforts With Canadian Agricultural Ministry and Canadian Company

...unola is able to offer health conscious consumers a unique natural alternative to petroleum-based ingredients. Natunola offers its customers vegetable gels based on canola, sunflower, castor and rice bran oils, as well as customized ingredients. About Kiwa Bio-Tech Products Group Corporation The Compa...

National Survey Shows Most Women Are Not Satisfied With Their Progesterone Treatment

...ies caused irritation and nearly half found them awkward to administer. A third experienced moderate to very severe side effects. Most women who took gels and vaginal capsules found them messy and leaky. Of the 11 percent (37 people) who used a progesterone gel, more than three- quarters experienced gel ...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

...ign, Marcel Dekker, New York, 1991. 52. E. K. Seto, S. Damaskinos, A. Dixon, W. L. Diehl-Jones, and C. A. Mandato, "Imaging electrophoretic gels with a scanning beam laser macroscope," Electrophoresis 16 , pp. 934-940, 1995. 53. A. E. Dixon, S. Damaskinos, A. Ribes, and K. M. Bees...

Evaluation of FlashPlate in a Helicase Assay

...t unwound DNA products be analyzed on polyacrylamide gels, which have major constraints in high throughput screening (HTS) applications. Preparing the gels is a time-, labor-, and material-intensive task, especially at high volume. FlashPlate is a 96-well polystyrene microplate with plastic scin...

Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers

...alysis 24-hour unattended operation Easy instrument set-up Convenience Minimal hands-on labor; no slab gels to prepare, no loading with pipettes, no glass plates to clean, no sample lanes to track Windows platform for analysis and results editi...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

... 17. 15 l of each PCR product were loaded on a 2% agarose gel and separated by 120 V for 25 minutes. gels are stained with ethidium bromide and visualized using the Gel Doc 2000 TM Gel Documentation System. Results The data prese...

Ettan DIGE Imager

...ttes for handling and scanning gels—cassettes are available for naked gels or membranes and DALT and SE 600 series gel sandwiches ... 11-0027-33 low-fluorescent glass, for naked gels

Imaging software ImageMaster 2D...

Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting

...roteins. Methods Pure proteins or cell extracts were run on 12% Trisglycine gels. After electrophoresis, gels were blotted onto a Hybond™ ECL or Hybond-LFP membrane, and then probed with primary antibodies and secondary CyDyeconjugated antibodies accordi...

ECL Semi-dry Blotters

...rm electrotransfer of proteins from polyacrylamide gels in less than 1 h • Minimal bu... TE 70 and TE 77 The TE 70 accommodates gels up to 14 × 16 cm, such as from the SE 400 an... series.

The TE 77 accommodates gels up to 21 × 26 cm, or four mini-gels side-by-...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

...annel images of Cy™2-, Cy3-, and Cy5 labeled gels and protein blots.

In this stud...3 with low-fluorescent glass, for naked gels Typhoon 9410 ...transferrin was loaded onto Novex 12% Tris-glycine gels in a series of two-fold dilutions from 5 ng to 0.6...

Extended data analysis using DeCyder EDA

... patient sample was run as a duplicate on separate gels that included dye swapping. The two spot maps from...ce. Standard spot maps from the master and matched gels (to provide matching information) were copied into...e duplicate patient were of very low quality. Both gels were from the same casting batch and showed severe...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...icular and parallel gels. Perpendicular gels have an increasing gradient of denaturants from ... direction of electrophoresis. Parallel gels have an increasing gradient of denaturants from to...to the direction of electrophoresis. Perpendicular gels are normally used to determine the opti...

Aurum Plasmid 96 Kit Tech Note, Rev A

...hotometry. All samples from the 4 OD culture were run on agarose gels to confirm purity and concentration of the plasmid. For each man...SA 76, 615619 (1979) Yang RCA et al., Elution of DNA from agarose gels after electrophoresis, Methods Enzymol 68, 176182 (1979) ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...sts were loaded onto 7.5% acrylamide (38.5:1) DGGE gels at hourly intervals. These travel gels were run in the DCode system apparatus at ...e apoA-I fragments and 4h for apoB. The gels were stained for 30 minutes in SYBR Green and ...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

...he DCode system at 100 V for 16 hr across a temperature range of 4055 C and a temperature ramp of 1 C/hr. After electrophoresis, gels were equilibrated in 1.25x TAE buffer for 15 min., stained in ethidium bromide (25 mg/ml in TAE) for 20 min. and washed in TAE b...

Heteroduplex Analysis Using the DCode System

...trand, causing a distortion in its usual conformation. This distortion or altered conformation can be detected on polyacrylamide gels due to slower migration than the corresponding homoduplex molecules. Heteroduplexes are generated during PCR* of a heterozygous ...

Detection of Mutations in the CYP21 Gene Using the DCode System

...ient ranging from 612% and a gradient of urea and formamide. The gels were run in 1x TAE buffer, at 80 V overnight. After electrophoresis, the gels were stained in TAE buffer containing ethidium bromide and subse...

Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

... through 0.45 m filters. Parallel-style gels were cast using the Model 475 Gradient Former (Bio-Rad, Hercules, CA). gels were run in a DCode unit in 1x TAE at 60 C for 912... h at 170 V (Bio-Rad Model 200/2.0 Power Supply). gels were subsequently stained with a 1:10,0...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

...separating gel poured to 5.5 cm height and a 1 cm high stacking gel consisting of 4% polyacrylamide. The separating and stacking gels were polymerized in a 37 mm diameter Model 491 Prep Cell casting tube assembly. Running Conditions The upper and lo...

Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

...e protocols address two key issues in applying 2-D gels to proteomics analysis of complex sampl...panded the capabilities of applying 2-D gels for proteome analysis of complex cellular extracts... Focused IPG strips were loaded onto 816% SDS-PAGE gels following equilibration for 10 min with...

Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A

...Figure 1) for running 2-D SDS-PAGE slab gels with preparative protein loads. The Dodeca ...nd reproducible set of second-dimension gels with very little smearing or streaking associated ...ls. Such is the importance of running high-quality gels for image analysis that many scientists...
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