gel

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

Brilliant Blue Dye Stratagene Cloning Systems, Inc. Stratagene currently provides an optical bandpass filter with all EagleEye II still video systems that is...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acidpurification kits Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, whichprovide reliable isolation of high-purity miniprep plasmid, PCR product andgel-fractionate...

CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

Stephen Roemer Mary Jensen Harry Osterman MarkVanek LI-COR Biotechnology Division LI-COR and Stratagene release the first commercially available precastgels for automated DNA sequencing and genetic analysis. Stratagenes CastAway gels* for...

Four New CastAway Precast Gels

CastAway precast gels* eliminate the need to manually pour gels, saving upto 4 hours preparation time. Stratagenes quality guarantee means no more gelslost to bubbles or leaking.All CastAway gels are guaranteed to maintainstability for a minimum of 60 days from the date of manufacture, with nodetectable deterioration in band resolution. 6% CastAway Gels with 32 Preform...

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

Optical Filter Optimized for Imaging Silver-StainedGels Stratagene II Still Video System that increases contrast...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

Gel Cleanup Kit George Halley, Eppendorf 5 Prime, Inc. Agarose gel electrophoresis is used extensively to separate and analyze DNA that is subsequently used for downstream applic...

Eppendorf Perfectprep Gel Cleanup Kit

Gel Cleanup Kit Blunt-end Cloning of DNA Isolated Using the Eppendorf Perfectprep Gel Cleanup Kit Eppendorf 5 Prime, Inc. October 1, 2002 Agarose gel el...

Eppendorf Perfectprep Gel Cleanup Kit

Gel Cleanup Kit Eppendorf 5 Prime, Boulder, Colorado The purity of DNA extracted from agarose gels can have a significant impact on downs...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Phase Lock Gel may be used in conjunction with TRIzol Reagent for the isolation of total RNA from cell and tissue samples. Increased yields are observed using this method, because the entire aqueous phase can be recovered without interphase contamination. Below is a protocol outlining the steps involved in RNA...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Phase Lock Gel may be used in conjunction with TRIzol Reagent for the isolation of total RNA from cell and tissue samples. Increased yields are observed using this method, because the entire aqueous phase can be recovered without interphase contamination. Below is a protocol outlining the steps involved in RNA...

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany The classic RNA isolation procedures are...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

Michael Hepburn and Glenn A. Miller, Ph.D., Molecular Profiling Laboratory, Genzyme, Framingham, MA The search for sequence variations in genomic DNA is important in the study of genes that play a role in the development of cancer and a variety of other single and multiple gene disorders...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

Martin A.O.H. Menke*, Axel Reinecke-Lthge*, Barbara Mllmann*, Angela Kellner, Jutta Lttges*, Gnter Klppel* * Department of Pathology and Department of Hematopathology, Christian- Albrechts University Kiel, Michaelisstrae 11, D-24105 Kiel, Germany. Introduction Genetic alterations/variations are...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

Terry H. Landowski, Ibrahim Buyuksal, and William S. DaltonH. Lee Moffitt Cancer Center and Research Institute, University of SouthFlorida, 12902 Magnolia Drive, Tampa FL 33612 Introduction Identifying of mutations within specific genes has become an important strategy in determining the diagnosis and prognosis f...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

Kristi M. Miller, Tobi J. Ming and Ruth E. Withler, Pacific Biological Station,Department of Fisheries and Oceans, Nanaimo, B.C. V9R 5K6, Canada Introduction We describe a rapid and sensitive method for the detection of nucleotide sequence variation which can be used for largescale screening of population...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

Tochigi Cancer Center, Tochigi, Japan Introduction Denaturing gradient gel electrophoresis (DGGE) is one of the most consistently used mutation scanning methods. It has evolved enormously over the last decade to be widely used particularly in gene diagnostic l...

Fractionation of Bovicola ovis Homogenates Using the Mini Whole Gel Eluter

Brian J. Shiell and Wojtek P. Michalski, CSIRO Division of Animal Health,Australian Animal Health Laboratory, Geelong, Victoria, Australia Introduction Amino acid sequence and composition analyses of proteins can be performed on samples derived from polyacrylamide gels. Protein electro-elution, the most c...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

, and Jef Raus , 1 Dr. L. Willems-Instituut and 2 Limburgs Universitair Centrum, Universitaire Campus, B-3590 Diepenbeek, Belgium Introduction Phenylketonuria (PKU) is a hereditary disease that gives rise to elevated blood leve...

Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

Bernd Eschweiler and Beate Kilb, Institute for Water Research, Schwerte, Germany Introduction Bacterial diversity in environmental samples is usually determined by a characterization of isolated strains. A problem for the analysis and characterization of microbial communities is the inability to culture...

Start-ups, angels can connect on new state Web site

Wisconsin entrepreneurs can submit executive summaries of their businesses for angel investors' perusal to a new state Web site beginning May 9. The The WAN website, which is being ca...

Angels step up

A fortnight ago, this column talked about the missing angels, those investors who provide the seed money that enable very early-stage companies to sprout. Predictably, I heard from lots of early start-ups who are looking for those angels. They confirmed what I wrote: It's difficult today to find investors willing to take even a small risk on a big, but unproven idea. Yet I also heard from a few i...

Where have all the angels gone?

Over the past month, I've seen a half dozen great products that may never see the light of the technology market. The products vary widely from unique hardware devices to much-needed vertical software applications to exciting new mobile services and beyond. Each concept is carefully conceived and well engineered. The market analysis has been done and each product meets a real and unfulfilled need...

Preparative Nondenaturing Gel Electrophoresis Used in the Purification of an Esterase Involved in Insecticide Resistance, Rev C

Dr Albert J Ketterman and SHP Parakrama Karunaratne, Dept. of MedicalParasitology, London School of Hygiene and Tropical Medicine, Keppel St,London WC1E 7HT UK Introduction In attempts to control Culex quinquefasciatus as a worldwide vector of diseases such and filariasis and encephalitis, mosquito field populati...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

Contributed by Philip W Miller, Waltraud I Dunn, and Robert R Schmidt, University of Florida, Dept. Microbiology and Cell Science, Gainesville, FL 32611 USA Florida Agricultural Experiment Station Serial Number T-00256 Introduction One of the research goals of this laboratory is to elucidate t...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

Menu B. Leddy, Biotechnology Research Department,Orange County Water District, 10500 Ellis Ave., Fountain Valley, CA 92708 Identification and characterization of bacterial species from membrane biofilms has been a microbiological challenge. A vast majority (>90%) of bacterial cells from membrane...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

M. E. Eberle, Wisconsin Regional Primate Research Center, University of Wisconsin, Universi...

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

The transfer efficiency of large (>100 kb) DNAs from agarose gels to membrane is poor unless the DNAs are nicked prior to transfer. Although this has traditionally been accomplished with HCl depurination, UV irradiation (254 nm) provides a faster and more consistent alternative to HCl nicking....

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

, Philip W. Wills , and Linda H. Malkas , (a) Department of Pharmacology and Experimental Therapeutics (b) Program in Molecular and Cellular Biol...

Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

Tech/Aid, 7700 Edgewater Drive, Oakland, CA 94621 USA, and Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Running PCR samples on agarose gels is a routine yet timeconsuming procedure....

Three Micron TSK-GEL ODS-100V Columns for High Throughput LCMS

It is well known in HPLC that columns packed with smaller particles are more efficient at the expense of higher back pressure compared to columns packed with larger particles. The gain in efficiency is usually traded off by reducing column length, which in turn results in shorter analysis...

Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80

Authors: T.J. Higley, Tosoh Bioscience LLC, Tatsunari Yoshida, Tosoh Corporation The TSKgel Amide-80 column provides excellent selectivity for separating peptides in hydrophilic interaction chromatography due to polar carbamoyl functionalities bonded to a silica base material....

Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns

While full coverage C18 phases have been responsible for the growth of HPLC as a reliable high efficiency technique, it has become apparent in recent years that such column types are not suited for reliable analysis of highly polar compounds under low organic or aqueous mobile phase conditions...

TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds

Using high purity Type B silica, Tosoh has introduced two new columns with differing surface treatment procedures and carbon content levels to provide unique selectivities for a wide range of sample types. In recent years, many new octad...

Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins

With the introduction of two new reversed-phase column types, TSKgel ODS-100V and TSKgel ODS-100Z, excellent chromatographic performance and selectivity can be achieved for a wide range of sample types. TSKgel ODS-100Z and TSKgel ODS-100V are based on a spherical, five micron, silic...

Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column

T.J. Higley and Roy Eksteen, Tosoh Bioscience LLC, Montgomeryville, Pennsylvania andScott Lauder, Lexigen Pharmaceuticals, Lexington, Massachusetts During method development, many variables are examined to ensure method robustness. Factors such as elution profile, peak shape, and recovery...

Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns

During a recent method optimization and investigation at Wyeth BioPharma, pre-treatment with bovine serum albumin improved reproducibility and recovery during the analytical SEC analysis of fractions pulled from a multi-ste...

Investor warns of angel-venture funding gap

Payne, an entrepreneur-in-residence for the and a prominent angel investor, will be in Madison to address the June...

Breaking down Wisconsin's angel capital numbers

The figures were outlined in a recent report titled Risk Capital in Wisconsin: A Progress Report for 2006, which was produced by NorthStar Economic...

TrafficCast secures $2M from Phenomenelle Angels, NEW Capital Fund

of Appleton.<...

Research explains why women lack equal access to angel capital

The research, conducted by Jeffrey Sohl, director of the at the University of New Hampshire, examined the equa...