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Counting Colonies or Plaques Directly from Images of Agar Plates

Michelle Cayouette Jane Moores Michael Steege AlanOrr Stratagene Cloning Systems, Inc. software version 3.1,researchers have the ability to automatically count bacterial colonies andplaques on agar plates. Researchers can use...

Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood

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Organize Your Data from the Big Blue Mutation Assay

Stratagene Solana Consulting Group, Solana Beach, CA Stratagene introduces the MutaTrak electronic notebook to record BigBlue transgenic rode...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acidpurification kits Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, whichprovide reliable isolation of high-purity miniprep plasmid, PCR product andgel-fractionate...

Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection

total RNA microprep kit for very small samples Stratagene Isolating total RNA from laser capture microdissection samp...

Simple Isolation of RNA from Tissue and Cultured Cells

Stratagene total RNA miniprep kit efficientlyisolates high-quality total RNA from 10 culturedcells or 10 to 40 mg of tissue and includes an on-column DNA-removal step.Additionally, multiple sa...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

Barry R. Neiditch Cherie Dewar Tanya Hosfield Marie Callahan John C. Bauer Mike Kobrin Quinn Lu Dianne Eckhardt Stratagene Human Genome Sciences, Rockville, Maryland Stratagene has developed the PCR Library Con...

Fast Isolation of Total RNA from Small Numbers of Cells

Extract total RNA from as few as 1 cell Stratagene total RNA microprep kit provides aconvenient method for isolating total RNA from small samples of cells. Forthe purification, RNA is bound to a solid support, which eliminates theneed for organic extraction and alcohol precipita...

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf<sup > Plasmid Mini Kit BioSystems Laboratory, Brinkmann Instruments, Inc., We...

Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. Buccal swabs are the least invasive way of collecting gDNA from humans, and also have the added benefit of minimizing the exposure to blood-borne pathogens. Buccal sw...

Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic DNA from 200 l of whole blood as described in the product manual. In addition,...

Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. It is not convenient in all instances to have blood drawn for gDNA extraction. The Eppendorf Perfect gDNA Blood Mini Kit was used to extract gDNA from...

Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. Buccal swabs are the least invasive way of collecting gDNA from humans, and also have the added benefit of minimizing the exposure to blood-borne pathogens. Buccal sw...

Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic DNA from 200 l of whole blood as described in the product manual. In addition,...

Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. It is not convenient in all instances to have blood drawn for gDNA extraction. The Eppendorf Perfect gDNA Blood Mini Kit was used to extract gDNA from...

Recovery of DNA from LMP Agarose1

Resolve DNA fragments on a Low Melting Point (LMP) agarose gel in 1x Tris-Acetate-EDTA (TAE) buffer. Tris-Borate-EDTA (TBE) is not recommended as TBE gels are much more difficult to solubilize. Stain gel with Ethidium...

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany The classic RNA isolation procedures are...

Genomic DNA Preparation from RNAlater Preserved Tissues

The following protocol is suitable for 100 mg tissue samples. Mouse kidney, lung, and spleen genomic DNA have been successfully extracted with approximate yields of 70 g DNA per 100 mg tissue....

Purification of Two Isoforms of Pyruvate Kinase from Nordic Krill Using an UNO Q1 Column, Rev A

Pyruvate kinase (PK, E.C. 2.7.1.40) is one of the key regulatory enzymes of the glycolytic pathway in crustaceans. This enzyme catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate and the phosphorylation of ADP to ATP. In addition to the role of PK in aerobic and anaerobic energy metabolism in...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. 1</...

Debate over `intelligent design' is a not-so-wise diversion from science education

- The day an archeologist finds a fossil cat or dog in Precambrian rock is the day Charles Darwin's evolution theory is disproved. But that hasn't happened. In fact, the Earth's fossil records tell a consistent scientific story through the ages: Today's living species descended -- with modification -- from common ancestors that lived in our anci...

Improve your networking skills by learning from other professionals

Without effective and proper networking techniques, you may not be maximizing your time while attending events or when reaching out to other professionals. While people have their own networking style that works for them, it may not work for others. You need to do what works best for you and your personality. At the same time, it never hurts to learn new ideas about networking from other...

Are women opting out or shut out from career tracks?

A new Harvard Business Review article, " On Ramps and Of...

Innovative Health Strategies near Milwaukee set to profit from Medicare expansion

Terri Bernacchi knows how to survive. The Milwaukee-area entrepreneur "bootstrapped" So now that IHS has begun to thrive, it is well positioned to help its pharmaceutical industry customers survive the massive new demands that...

The Creative Class from my perspective

Rich Florida has taken a lot of crap from the academic community for the theory he vetted in his best-seller, The Rise of the Creative Class. To summarize, Dr. Florida says that a citys predisposition for Talent, Tolerance and Technology are indicators of economic boomtowns. Some of Richs indexes are controversial. For example, Rich shows that the higher a citys concentration of gays, t...

Nebraska bank benefits from Fiservs integrated solutions

Brookfield, Wis. A Midwestern community bank can now remove the guesswork out of customer profitability analyses, based on the integration of two technologies from two separate divisions of Wisconsin-based Fiserv. , located in Laurel, Nebraska, can now view financial relationship data from any of its desktops and ident...

Industry Veterans take a look back from 2025: Bio-Technology, and Bio-Informatics are the focus

SCOTTSDALE, Ariz. -- Panelists at the Demo 2003 conference offered insights into the next technology innovations by answering Producer, Chris Shipley's question, "If we look back in the year 2025 what will see as the big innovations?" Mitch Kapor, Chairman of the Open Source Applications Foundation, and founder of Lotus, began the discussion by stating, "I would be hanging out in the Bio...

Whole Genomes From Microbes to Man

Madison, WI-- Optical Mapping is a new technology that will be used to discover why patients experience toxic side effects, or fail to respond to medication and to identify the causes of antibiotic resistance and virulence in pathogenic organisms. Wisconsin- based OpGen, has announced the commercial launch of its Optical Mapping(TM) system for rapid, cost-effective analysis of whole genomes, from...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

Gene expression profiling of material isolated by microdissection hasbecome a very popular method for analyzing cellular behavior in a microscale and is used in a wide range of research and clinical applications.Laser-assisted microdissection allows isolation of...

Protocol for GenomePlex Whole Genome Amplification from Buccal Swab

Application Guide I. II. III. IV. V. VI. <a href="#VI"...

Protocol for GenomePlex Whole Genome Amplification from Blood Card

Application Guide I. II. III. IV. V. VI. Quantificat...

Protocol for GenomePlex Whole Genome Amplification from Plant

Application Guide I. II. III. IV. V. VI. Quantification of Amp...

Protocol for GenomePlex Whole Genome Amplification from Soil

Application Guide I. II. III. IV. V. VI. Quantification of Amplifie...

Protocol for GenomePlex Whole Genome Amplification from Whole Blood

Application Guide I. II. III. IV. V. VI....

Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue

I. Desc...

A Better Way to Isolate RNA AND Protein From the Same Sample

Isolate nuclear and cytoplasmic RNA and protein from cultured cells Ambion's new PARIS Kit uses a fast, simple procedure based on Ambion's RNAqueous technology to isolate bo...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

Maximize Viral RNA Yield from Biological Fluid

Ambions magnetic bead-based MagMAX Viral RNA Isolation Kit is optimized for isolating viral RNA from biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. RNA isolation from cell-free samples presents some unique challenges compared to RNA...

Isolate Total RNA and Protein From the Same Sample

Vana PARIS Kit combines the technological advances from both the <A href="http://www.ambion.com/catalog/CatNum.php?1560">mir <A href="http://www.a...