develop

RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

Gene arrays can be used to simultaneously measure the expression levels of thousands of genes in a single experiment. One limitation of this technology is that large amounts of RNA may be required for each array hybridization experiment -- typically 10 g of total RNA. This becomes problematic when working with limited sampl...

Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

Fang-Fang Wu, Xuemei He, and David R Nau, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA Immobilized metal affinity chromatography (IMAC) is a powerful technique that can be used for the efficient purification of recombinant histidine-tagged (His-tagged) proteins from a variety of ex...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

Michael Hepburn and Glenn A. Miller, Ph.D., Molecular Profiling Laboratory, Genzyme, Framingham, MA The search for sequence variations in genomic DNA is important in the study of genes that play a role in the development of cancer and a variety of other single and multiple gene disorders...

Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.

Recent advances in combinatorial chemistry and genomics programs have led to an unprecedented increase in both the number of compounds and potential drug targets that can be screened. The development of automated liquid h...

Who owns software developed for your company?

Even when there is a written contract, the specifics of ownership of the intellectual property in the software are often no...

Covance Appoints Co-Presidents for Early Development Services

PRINCETON, NJ - Covance Inc. has promoted Wendel Barr to the position of president, early development, in Madison, Wis., and Anthony Cork to the position of president, early development, in Harrogate, UK. The company said the move supports the regional decision-making employed by its clients in the preclinical segment. With the promotion, Wendel and Tony have become corporate senior vic...

The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis

Drs. Frank Slack, Steven Johnson, and Helge Grosshans, and Ms. Kristy Reinert at Yale University, in collaboration with Ambion scientists, discovered that the microRNA (miRNA) family regulates the expression of C. elegans...

Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System

LaRene Kuller, David Anderson, Dominic Andrada, and Richard Grant, NationalPrimate Research Center, University of Washington, Seattle, WA 98195 USA We have been using four separate enzyme-linked immunosorbent assays (ELISAs) to screen our primate colony for the viruses of greatest concern: simian...

The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System

CXCR2 • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads Chemo-attractant cytokines (chemokines) are low molecular weight, pro-inflammatory cytokines with potent leukocyte activation and/or chemotactic activity (1). Chemokines are classified into four families (CXC, CC, C, and CX...

The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

Melanocortin • receptor binding assay • LEADseeker • SPA Imaging Beads Pro-melanocortins are present in high levels in the pituitary and are processed into three major peptide hormone fragments: adrenocorticotrophin (ACTH), the melanocyte stimulating hormones, and β-endorphin. The various melanocyte stimulat...

The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System

Melanocortin • receptor binding assay • LEADseeker • SPA Imaging Beads Pro-melanocortins are present in high levels in the pituitary and are processed into three major peptide hormone fragments: adrenocorticotrophin (ACTH), the melanocyte stimulating hormones, and β-endorphin. The various melanocyte stimulat...

The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System

CGRP • receptor binding assay •LEADseeker • SPA Imaging Beads Calcitonin-gene related peptide (CGRP) is believed to have an important physiological role in the central nervous and cardiovascular systems. Indeed, using CGRP receptors isolated from coronary and basilar arteries, studies have i...

Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

neurite outgrowth • cell-by-cell • fluorescent image analysis • IN Cell Analyzer • IN Cell Developer Toolbox Neurite extension, or neurite outgrowth, plays a fundamental role in embryonic development, neuronal differentiation, and nervous system function. Neurite outgrowth is also critical in some neuropatho...

Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System

receptor • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads Histamine is a biogenic amine that plays important pathophysiological roles in central and peripheral tissues. Generally released from stimulated mast cells, it contributes to several biological processes that characteri...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

G protein-coupled receptors (GPCRs) are the largest family of transmembrane receptors and are responsible for the transduction of a diverse range of extracellular signals. Up to 40% of clinically ma...

Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System

Neurokinins, also known as tackykinins, are a family of small neuropeptides. Members of this family include peptides, substance P (SP), neurokinin A (NKA), neurokinin B (NKB), and the recently discovered hemokini...

The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System

Somatostatin (SS) is a neurotransmitter/hormone with a wide range of biological actions (1–3) and a reduction in cortical SS levels has been reported in Alzheimer’s and Parkinson’s diseases. The SS analog...

The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System

In mammals, serotonin (5-hydroxytryptamine, 5-HT) is a regulator of neural transmission which induces its biological effect by binding to serotonin receptors. <P >The serotonin 5-HT rece...

Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

The α +...

Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System

In the mammalian brain, the corticotrophin-releasing factor (CRF) system comprises four CRF-related peptides (CRF and urocortin 1, 2, and 3) and two G protein-coupled CRF receptor subtypes (CRF...

IT Task Force to examine Workforce Development projects

The hearing, which will focus on three Department of...

Phillips Plastics to help develop orthopedics with stem cells

The agreement with BioE, Inc., which develops human ste...

Mirus Bio secures $900K grant to develop research tool

Mirus, a Madison-based life-science company engaged in gene therapy and the study of RNA interference, received the two-year grant from the <a href="http:/...

Alzheimer's drug developer lands $1.2M in venture funding

The latest round of funding brings the total equity capital raised in t...

IT workforce development: A little workforce planning goes a long way

Editor's note: . - If the estimates are accurate, about 10 percent of all information technology positio...

Of private equity, research, and drug development

, for $29 billion. The New York Times <a href="http://www.nytimes.com/2007/04/03/business/03data.web.html?_r=...

IT workforce development: Losing the numbers game

- John Psuik isn't sure whether expanding into new markets will significantly impact...

Milwaukee software developer gains bottom line, loses waistline

And now, the route of that marathon has taken a sharp turn toward Europe and Asia. Psuik, founder and owner of <a href="http://www.developerone.c...

WMEP wins top economic development award

The award, presented at th...

Scott Converse, UW-Madison School of Business, on IT workforce development

is at the forefront of trends in information technology workforce development. In part one of this interview, he talks about the market need for courses that help IT professionals in their progression f...

Madison life-science firms agree to co-develop kidney product

, have entered into a license and supply agreement to commercialize products to treat...

BioSystem Development secures angel funding

BioSystem Development, LLC</...

Renovar captures $1.2M for product development

, which has raised a total of $3.2 million since its inception in 1999, will use the proceeds from its second financing ro...

Cray signs $250M deal to develop supercomputer

However,...

Grants worth $800,000 help BellBrook Labs develop products

The biotechnology company, which develops high throughput screening tools for the drug-therapy market, said the new grants include Phase I and Phase II (SB...

Going for the "Big Score" or hedging your bets: Two economic development strategies

announced his state would spend $300 million to convince a major biomedical institute to open a center in Or...

Invitrogen boosts drug development capability

The new capability will be made possible by 's recent acquisition of Sentigen Holding Corp., which will be integrated with Inv...

Don't forget UW's role in economic development

Or those candidates ca...

Conference to address small town development

The conference is scheduled to take place on Sept. 20 from 8 a.m. to 5 p.m. and Sept. 21 from 8 a.m. to noon at th...

Fiber-optic infrastructure spurring city economic development

While some municipalities are fighting AT&T about Project Lightspeed and others are looking at Wi-Fi applications, others are looking at major fiber-optic investments. While these fiber investments have paid off in Utah as we mentioned in last week's column, there are others that we don't hear much about in the Midwest. As one CIO in Jacksonville, Fla. <a href="http...