cycle

Automated Fluorescent-Tag Cycle Sequencing

Stratagene Cloning Systems, Inc. *,# with Hot Top Assembly can be used to perform fluorescent-tag cyclesequencing using the ABI PRISM dye terminator cycle sequencingr...

Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results

Michelle Cayouette Jane Moores Jason Capodanno Michael Rahilly Stratagene produces consistent PCR results in every well. The RoboCycler systems uniquedesign uses thermal blo...

New Temperature Cycler Features Interchangeable Blocks

Michelle Cayouette Jane Moores Jason Capodanno Michael Steege Michael Rahilly Stratagene Infinitytemperature cycler, a new temperature cycler withinterchangeable blocks for...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In man...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

Peter Scheinert, PhD, Barbara Behrens, PhD, and Dietmar Kahle, PhD Peter Scheinert, PhD, Bernhard-Nocht-Institute for Tropical Medicine, Virology Dept., Bernhard-Nocht-Str. 74, D-20359 Hamburg former address: University of Kiel, Institute for General Microbiology, Am Botanischen Garten, D-24118 K...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

Using Eppendorf Mastercycler gradient NPC Laboratory, Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia...

The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis

Karen Sermon, M.D., PhD Center for Medical Genetics, University Hospital and Medical School of the Dutch-speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium Preimplantation diagnosi...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In many research projects, cD...

Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

From MAIN MENU go to FILES/ EDIT/ STANDARD rewrite accordingly then EXIT/ YES (use SEL to choose)/ ENTER/ change name to BIGDYE/ ENTER...

Mastercycler Gradient Quick Programming Guide

USE ARROW KEYS TO SCROLL THROUGH MENUS AND PROGRAMS. PRESS ENTER TO SELECT HIGHLIGHTED CHOICE) Menu items are chosen by first positioning cursor to the choice desired, then press . This confirms choice or input data or changes display to next menu in hierarchy....

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are! Choose the LightCycler PCR System, the only instrument flexible enough to meet your changing research needs. Central to the LightCycler System is the LightCycler Instrument which is much more than "Just an...

Biotech industry reaches midpoint in 50-year maturation cycle

An interesting article in ) on March 1 states unequivocally that most industry cycles run about 50 years until they mature. If this...

Screening of Beer-Spoilage Bacteria Using the LightCycler PCR Workflow System

The new LightCycler foodproof Beer Screening Kit (dedicatedto the detection of beer-spoilage bacteria on theLightCycler Instrument) in combination with the MagNAPure LC DNA III Kit (for the automated isolation of bacterialDNA on the MagNA Pure LC Instrument) offersbr...

LightCycler foodproof Beer Screening Kit

Breweries make continuous efforts to ensure the highestquality of their goods. To guarantee the consistency ofproduct quality, the different stages of beer production...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

Since the first description of RNA interference (RNAi) asa protection mechanism against invasion of foreign genesappeared a few years ago, RNAi has become a powerfultool for analyzing gene function in mammalian cells. RNAiis mediated by short double-stranded RNAs that a...

Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS

In human cells, the enzymes thymidin phosphorylase(TP), dihydropyrimidine dehydrogenase (DPD), and thymidylatesynthase (TS) play a pivotal role in the metabolismof pyrimidines. In cancer disease medical care, thecytotoxic compound 5-fluorouracil (5-FU) is the standard...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

Faye Boeckman, Larissa Tan, Marni Brisson, Rob Park, and Keith Hamby,Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 When researchers need to measure the amount of RNA or DNA in a preparation, they typically employ a traditional biochemical approach. High concentrations of...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

Marni Brisson, Larissa Tan, Rob Park, and Keith Hamby,Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 USA Real-time PCR* is a powerful and effective technique for accurate quantitation of DNA. Assays for the detection of a single gene involve careful choice of primers, tar...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

Giles Cunnick and Wen Guo Jiang, Metastasis Research Group, UniversityDepartment of Surgery, University of Wales College of Medicine, Cardiff, UK Lymphangiogenesis refers to the formation of new lymphatic vessels. This process may occur in normal developing tissues or in tumors. In addition to sprea...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

Departments of Ophthalmology and Cell Biology, Duke University Medical Center, Durham, NC 27710 USA Correspondence: Catherine Bowes Rickman, PhD, Department of Ophthalmology, Duke University Medical Center, Box 3802, Durham, NC 27710, Phone (91...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

130 West Kingsbridge Road, Bronx, NY 10468 USA Schizophrenia is a severe mental illness that afflicts about 1% of the population (Strange 1992). Patients experience a variety of symptoms that have been divided into subgroups...

Real-Time Immuno-PCR on the iCycler iQ System, Rev A

Kristina Lind and Mikael Kubista, TATAA Biocenter, Medicinaregatan 7A/B,405 30 Gteborg, Sweden A technique for antigen detection, called immuno-PCR, was developed by Sano et al. (1992). It combines the molecular recognition of antibodies with the high DNA amplification capability of PCR. The procedu...

The iCycler iQ Detection System for TaqMan Assays, Rev B

Faye Boeckman, Keith Hamby, and Larissa Tan, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Specific fluorescent oligonucleotide probes allow for real-time monitoring of the polymerase chain reaction (PCR*). One popular probe strategy is the TaqMan assay...

The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

Andrew Tingey, Reading Scientific Services Ltd., The Lord ZuckermanResearch Centre, Whiteknights, PO Box 234, Reading RG6 6LA, UK One of the most topical issues in the food industry is the use of genetically modified organisms (GMOs) in agriculture and food production. Demands from consumers, particu...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

M. Kenrick, S. Hancock, S. Stubbs, and N. Thomas GE Healthcare, The Maynard Centre, Cardiff, UK Recent advances in siRNA methodologies and the development of high-throughput image analysis platforms such as the IN Cell Analyzer have revolutionized the functional analysis of genes and proteins. Here, we describe the application of...

Quantitative Analysis of the Cell Cycle in Imaging Applications

Quantitative analysis of stages in the cell cycle is commonly used in cell biology since many pharmacological interventions aim to modulate the cell cycle in some way. We present here an application that can be used in adherent cells and optionally multiplexed for the use in automated cell signaling analysis and drug-screen...

Technology cycles from advantage to necessity to disadvantage

There is a continual cycle for o...

Pacific Cycle rides full speed into RFID

The company, which owns such traditional brands as Schwin...

Is venture capital dead? No, but its beginning a new cycle

Howard Anderson, who teaches courses on early-stage companies at the Massachusetts Institute of Technology, founded or co-founded three venture capital funds. He was a self-described financial samurai. Now, hes hanging up his sword, declining to raise new money for his funds. Theres too much venture money pursuing too few deals. Theres nowhere for all that money to go: we cant spend the...

Luem Software and Trek Bicycle sign exclusive software agreement

Genoa, Wis. to license Leums Ascend retail management software package. Trek and Leum Software intend to enhance the current Ascend software to provide Trek dealers with additional tools...