Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
midiprep kit Stratagene EF plasmid midiprep kit, a kit based on anion exchange chromatography, magneticbeads, and cesium ch...An Epitope Tagging Vector for Gene Expression in Mammalian Cells
Stratagene The pCMV-Tag1 vector is an epitope tagging vector designed for geneexpression in mammalian cells. A target gene inserted into the pCMV-Tag1 vectorca...Big Blue Rodent Cell Cultures for Mutagenesis Studies
Rat and mouse embryonic cell lines are useful for in vitro mutagenesisstudies Stratagene Cloning Systems, Inc. The Big Blue rat cell line and the Big Blue transgenic mouse embryoniccell line are useful for in vitro mutagenesis studies. These cell lines are partof the Big Blue transgenic rodent mutagenesis assay system...Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
Stratagene mammalian expressionsystem allows tightc...Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
Quantitating cell number is more accurate using the Quantos cellproliferation assay kit Genetic Applications LLC cell proliferation assay kitprovides a simple, reproducible method to assess the number of cells present invarious tissue culture dish formats. In this st...XL10-Gold Cells Supplied in New Single-Reaction Format
solopack Gold cells reduce the time and effort needed fortransformationswithout compromise! Anna Waesche Jonathan Goodwin Amy Duncan BruceJerpseth Alan Greener Stratagene strain* in an easy-to-use, single-reaction format thatyield th...Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines for...Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
total RNA microprep kit for very small samples Stratagene Isolating total RNA from laser capture microdissection samp...Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Department of Cell Biology, Neurobiology, and Anatomy University of Cincinnati College of Medicine, Cincinnati, OH 45267 Transfection of mammalian cell lines is enhanced by the use ofliposome-based reagents. Here we compare the transfection e...Simple Isolation of RNA from Tissue and Cultured Cells
Stratagene total RNA miniprep kit efficientlyisolates high-quality total RNA from 10 culturedcells or 10 to 40 mg of tissue and includes an on-column DNA-removal step.Additionally, multiple sa...Enzyme Immunoassay for Studying Intracellular Levels of cAMP
Sensitive, nonradioactive cyclic-AMP detection kit and adenylate cyclase(AC) toxin Stratagene Cloning Systems, Inc. American Qualex Stratagenes EIA cAMP Kit is a highly sensitive and specificantibody-based assay kit for no...New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
Carsten-Peter Carstens Julie Bonnardel Anna Waesche Ronda Allen Stratagene Codon bias is a significant obstacle for efficient expression ofheterologous genes in E. coli hosts. In GC-rich genomes, such as mammals,rare arginine codons (AGG or AGA) and the proline codon (CCC) most fre...TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
Institute for Molecular Biology and Biotechnology, McMaster University,Ontario, Canada GST-fusion proteins expressed in TKB1 bacteria are used to affinity purifycellular proteins, which interact with a specific autophosphorylation site onthe Erb...Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
Stratagene Streamline RNA analysis with a whole host of products from Stratagene.First, the StrataPrep total RNA miniprep kit isused to isolate ample yields of high-quality RNA from tissues and cells, thenthe RNA is r...A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
Genetic Applications LLC transfection reagent canbe used for simple and reproducible delivery of foreign DNA into mammaliancells. In this study, we used varying amounts of LipoTAXI transf...Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
Stratagene Cloning Systems Clonetics Corporation, Walkersville, Maryland LipoTAXI transfection reagent is a novel liposome reagent that offerslow cellular toxicity and high transfection efficiencies for adhe...Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Mycoplasma PCR Primer Set for fast and easy detection of mycoplasmacontamination Yale University Genetic Applications LLC Stratagene Mycoplasma contamination of mammalian cell cultures can substantiallyreduce the potenc...New Competent Cells for Highest Transformation Efficiencies
Stratagene Cloning Systems, Inc. Stratagenes new, high-efficiency competent E. coli cell linesignificantly improves the transformation efficiency of ligated DNA. EpicurianColi ultracompetent cells* yi...Fast Isolation of Total RNA from Small Numbers of Cells
Extract total RNA from as few as 1 cell Stratagene total RNA microprep kit provides aconvenient method for isolating total RNA from small samples of cells. Forthe purification, RNA is bound to a solid support, which eliminates theneed for organic extraction and alcohol precipita...Cis-Reporting System Monitors Intracellular Calcium Mobilization
Stratagene cis-reporter plasmid,the pNFAT-Luc reporter plasmid, is designed for studying the activation of thenuclear factor of activated T-cell (NFAT) transcription factors...Cis-Reporting System Monitors Intracellular Calcium Mobilization
reporting system for studyingNFAT activation Stratagene cis-reporterplasmid, the pNFAT-Luc reporter plasmid, is designed for studying the activationof the nuclear factor of activated T-cell (NFAT) tr...CELLocate protocol blocks for 175 m microgrid
...Printable CELLocate Log Sheets for Easily Locating Adhererent Cells
Log Sheets for Easily Locating Adhererent Cells CELLocate is the ideal solution for relocating adherent cells that have been used for microinjection. These sterile coverslips come with a microgrid pattern. Using the CELLocate proto...CELLocate protocol blocks for 55 m microgrid
...Principles and applications of electrical cell fusion
Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...Principles and applications of electrical cell fusion
Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany The classic RNA isolation procedures are...Primary cells, rat heart endothel
Primary cells: Microvascular endothelial cells from...Primary cells, rat heart muscle
Primary cells: Adult heart muscle cells from rat, 6...Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
Martin Simon, Petra Albrecht, Helmut J. Schmidt, and Andrs Jarrin Martin Simon, Petra Albrecht, and Helmut J. Schmidt, University of Kaiserslautern, Germany, Department of Ecology Andrs Jarrin, Eppendorf AG With the help of the Eppendorf cM...The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis
Karen Sermon, M.D., PhD Center for Medical Genetics, University Hospital and Medical School of the Dutch-speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium Preimplantation diagnosi...The QIAGEN Guide to Animal Cell Culture
Welcome to a new series of articles aimed at providing useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects ). This article introduces different types of animal cell cultures. The series...