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Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

Stratagene mammalian expressionsystem allows tightc...

Highest Possible Transformation Efficiencies for High-Throughput Applications

Convenient, time-saving 96Pack Gold competent cells streamline transformation procedures Julie Bonnardel Ronda Allen Bruce Jerpseth Sean Banks...

New Temperature Cycler Features Interchangeable Blocks

Michelle Cayouette Jane Moores Jason Capodanno Michael Steege Michael Rahilly Stratagene Infinitytemperature cycler, a new temperature cycler withinterchangeable blocks for...

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

hrGFP expression vectors for monitoring gene expression and localizing proteins in vivo New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter...

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice orRats Precise and cost-effective micronucleus analysis made easy with theicroFlow Micronucleus Analysis Kit Litron Laboratories, Rochester, NY Mark J. Dycaico<br...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines for...

Highly Active and Removable AffinityTagged Lambda Phosphatase

Dualspecificity Lambda Phosphatase useful for dephosphorylation serine,threonine or tyrosine residues Stratagene Cloning Systems, Inc. Stratagene's recombinant Lambda Phosphatase is a dualspecificity proteinphosphatase that has been produced as a fusion to Stratagene's novel affinitytag, the calmodulin...

High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications

Simultaneous purification of 96 PCR products Stratagene Phenogenex 96 PCR purification kit is a newmember of the StrataPrep product line. This kit offers a high-throughput formatfor purifying ultra-pure PCR product DNA from la...

Mammalian Expression Vectors with Interchangeable Selectable Markers

Site-specific recombinase-based vector system Stratagene system of eukaryotic expression vectors offers unparalleled convenience. The drug-resistance genes of these vectors can be exchanged without subcloning. Through site-specific recombination, hygromycin, puromycin,...

Printable CELLocate Log Sheets for Easily Locating Adhererent Cells

Log Sheets for Easily Locating Adhererent Cells CELLocate is the ideal solution for relocating adherent cells that have been used for microinjection. These sterile coverslips come with a microgrid pattern. Using the CELLocate proto...

Chemical Stability of Disposables

Disposables such as Eppendorf Tubes and pipette tips are frequently exposed to chemical reactions and they must withstand the various kinds of stress and strain encountered in everyday laboratory work (such as centrifuging and autoclaving). The stability level of these products is largely determined by the materials fr...

Efficient and Reliable PCR Setup Using Eppendorf MasterMix

Christian Rohrer, Eppendorf AG, Germany Lars-Erik Peters, Eppendorf 5Prime, USA The reliability of a PCR* experiment is dependent on its sensitivity, specificity and reproducibility. An essential prerequisite for a succ...

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are! Choose the LightCycler PCR System, the only instrument flexible enough to meet your changing research needs. Central to the LightCycler System is the LightCycler Instrument which is much more than "Just an...

Reverse transcriptases suitable for one-step RT-PCRb

city Tolerance<...

Reverse transcriptases suitable for two-step RT-PCRd

GC-rich...

A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

β -lactamases are either plasmid or chromosomally encoded bacterial enzymes which hydrolyze β -lactam antibiotics. The majority of β -lactamases produced by clinical isolates are serine active site enzymes. One successful approach to combating the serine active site β -lactamases is the use of β -lactamase inhibit...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

The TempliPhi DNA Sequencing Template Amplification Kit is designed to eliminate...

It's not Y2K, but changes to Daylight Saving Time could cause tech problems

will have significant implications for organizations worldwide, but few business organizations are prepared to address their potential information technology impacts, according to a new <a href="http://www.gartner.com/DisplayDocument?ref=g_search&id=500284&subref=simplesea...

Many still distrust Web sites is yours credible enough?

Many Internet surfers often believe they know a good Web site when they see one. Their intuition is based on a number of surface-level factors. For example, surfers are more likely to trust a site when it a...

Sanyo to advance Rayovacs rechargeble batteries

MADISON - Sanyo will be working with Rayovac's I-C3 technology, which the company says can recharge Nickel Metal Hydride, or NiMH, batteries in 15 minutes. I-C3...

Wireless Are you at Risk and Liable?

Recently, I was at a security conference and stayed at a hotel, which promoted wireless Internet service for $9.95 per day. They claimed that they had implemented it so only those on the first floor could get wireless Internet access. I do not purchase wireless Internet service when Im at seminars because I usually get access to my network at the seminar hosts office. Out of curiosity,...

Distance Education Conference Selects Sonic Foundry to Make Keynotes, Panel Presentations Available On-Demand

MADISON, WI Aug. 11 Sonic Foundry a rich media solutions company, today announced the 19th Annual Conference on Distance Teaching and Learning will use its Mediasite Live rich media Web presentation system to capture the keynote addresses and panel presentations, and make them available on-demand via the Web and on CDs containing this year's conference proceedings. Held Aug. 13-15 at th...

Detecting Your Companys Early Trouble Signs

CHICAGO, IL Growing up, "life turns on a dime" was one of my mother's favorite expressions. She always told me that life could change in the blink of an eye. Personally, this happened to me in May 1995 when I woke up and had blurry vision. A few days later, I was diagnosed with diabetes. That day changed my life forever. Business also turns on a dime. It's a constant roller c...

Diamond Film May Enable Critical New Sensors For Bioterror

MADISON - In this time of the chronic threat of terrorism and the possibility of war with an adversary who may be armed with biological weapons, high on the wish list of security agencies and battlefield commanders is a quick and easy way to detect the presence of dangerous biological agents. Now, with the help of a novel diamond film developed by chemists at the University of Wisconsin-...

University Consortium Enables Transformation and Survival for Wisconsin Businesses

Madison, WI - Todays business climate is not for the faint of heart. Global competition and offshore manufacturing are striking a blow to Wisconsins economy, and this, combined with rising unemployment, the need to retrain workers, and a huge budget deficit is forcing Wisconsins business leaders to look for new ways to survive and grow. If youre in business, youd better start thinking a...

Trouble-shooting: Low yield

Here are some troubleshooting hints that we have gathered regarding low yield after PCR reactions: <a href="#Option1">Primer annealing temperature is too high...

Trouble-shooting: Non-specific bands

Here are some troubleshooting hints that we have gathered regarding non-specific bands after PCR reactions: <a href="#Option1">Non-optimal Mg<s...

Trouble-shooting: Misincorporation or low fidelity

Here are some troubleshooting hints that we have gathered regarding misincorporation or low fidelity of PCR reactions: <a href="#Option1">Magne...

Trouble-shooting: No product

Here are some troubleshooting hints that we have gathered if your PCR yields no product: Non-optimal Mg...

Trouble-shooting: Smeared bands

Here are some troubleshooting hints that we have gathered regarding smeared bands after PCR reactions: <a href="#Option4">Primer annealing temperature is too low...

PCR-Grade Nucleotides Larger Pack Sizes Now Available

Roche Applied Science now offers new larger pack sizesof PCR-Grade Nucleotides. These larger pack sizescombine the best available quality with attractive pricing. Single dN...

The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling

In general, oligonucleotide microarrays are composed ofa two- or three-dimensional solid surface where DNA,used as a capture molecule (probe), is attached in anorderly fashion. Each of the oligonucleotide probes onthe microarray corresponds to a specific target gene,...

Efficient and Reliable Linear Amplification of cRNA

Gene Expression Profiling Using Microarray Kits from Roche Applied Science The use of gene-expression profiling, a genomic applicationmeasuring mRNA transcript levels of many genes inparallel, has been growing rapidly...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins from total lysates. There are several commercially available resins but all of them s...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

RNAqueous-Micro Kit Ideal for Processing Laser Capture Microdissection Samples Further development of Ambion's RNAqueous-Micro Kit has resulted in a 3-6 fold increase in total RNA yields from LCM samples. In addition, Ambion has introduced a new LCM Staining Kit that has been o...

siRNA Expression Vectors with Selectable Markers

siRNA expression vectors are now available, each with an antibiotic resistance gene to facilitate selection in mammalian cultured cells. These new vectors are ideal for the long-term study of gene specific kno...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

Departments of Ophthalmology and Cell Biology, Duke University Medical Center, Durham, NC 27710 USA Correspondence: Catherine Bowes Rickman, PhD, Department of Ophthalmology, Duke University Medical Center, Box 3802, Durham, NC 27710, Phone (91...

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

James R. Wong, Ph.D. and Wei-Yu Lin, Bio-Rad Laboratories A double pulse format for electroporation of mammalian tissue culture cells gave lower transient expression than a single exponential decay pulse. Moreover, separate administration of the two components of the double pulse rev...

Oragene is compatible with TaqMan SNP genotyping

DNA Genotek Inc., Ottawa, ON, Canada DNA collected with Oragene works well with TaqMan SNP Genotyping Assays. SNPs in the thymidylate synthetase and apolipoprotein E genes were reliably detected. Single-nucleotide polymorphisms (S...

Fast Multi-Residue Pesticide Analysis in Soil and Vegetable Samples

The strict detection limits that are required for the quantitation of pesticide residues in food and environmental samples requires the use of very sensitive, selective, and rugged LC/MS/MS technologies. In this application note the benefits of using LC/MS/MS for this type of analysis...