bioanalyzer

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to determine the efficiencyof transfection of mammalian cells using green fluorescent protein(GFP) as a reporter molecule. Transfection of CHO-K1 cells with...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer canbe used to analyze apoptotic cell samples. Performance of two assaysfor apoptosis detection is demonstrated and the data are compared tothose obtained with a conventional flow cytometer. Histogram quality...

Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer

A simplified and fast protocol for the analysis of DNAfragmentation during apoptosis This Application Note describes a simplified and fast protocol for apoptosisDNA fragmentation analysis with the Agilent 2100 bioanalyzerusing the DNA...

Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining

A fast and accurate method to detect green fluorescent proteinexpression in samples of low cell numbers This Application Noteaddresses the major problemsthat many researchers workingwith primary cells have, for example,limited availability, quan...

Agilent 2100 Bioanalyzer Automated Analysis System

Powered by major advances in microfabricationtechnology, the developmentof a fully automated laboratory on a chipis now a reality. Designed to integratesample preparation, fluid handling andbiochemical analysis, "lab-on-a-chip"is revolutionizing life science resea...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to detect cell surfaceand intracellular protein targets by antibody staining. CD3 staining onJurkat cells as well as CD4 staining on CCRF-CEM cells and intrace...

2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer

Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer The Agilent 2100 bioanalyzer is aunique, on-chip, electrophoresis andflow cytometry analysis tool for RNA,DNA proteins and cells....

siRNA transfection optimization with the Agilent 2100 bioanalyzer

When working in the field of target validation, or when studying functionalassays requiring the transfection of small interfering RNA (siRNA)into a cell, the presence of having a fast, easy-to-use method to optimizetransfection conditions for given cell lines would be of g...

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

This application note describes how the Agilent 2100 bioanalyzer canbe used in combination with Standardized RT (StaRT)-PCR reagentsfrom Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville,AL, USA) to obtain highly reproducible, standardized gene expressionme...

Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay

Antibodies are commonly used in diagnostics, as research tools(for example protein arrays) or as biopharmaceutical therapeutics. Inall cases the successful production of antibodies requires precise testingand quality control. The key quality criteria include: protein ident...

Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis

Whereas in the past mass spectrometers (MS) were confined to therealm of small molecules, the development of soft ionization methodssuch as matrix-assisted laser desorption/ionization (MALDI) and electrosprayionization (ESI) revolutionized the analysis of large biomolecule...

Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer

Duplications and deletions are known to cause a wide variety of humandiseases. Many different methods are used for detecting such rearrangements,most based on either FISH or the quantitative analysis of PCR products.FISH is popular and accurate, but very labour intensive a...