bioanalyzer in Biological Technology

Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis

Whereas in the past mass spectrometers (MS) were confined to the realm of small molecules, the development of soft ionization methods such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization...

Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay

Antibodies are commonly used in diagnostics, as research tools (for example protein arrays) or as biopharmaceutical therapeutics. In all cases the successful production of antibodies requires precise testing and quality c...

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

This application note describes how the Agilent 2100 bioanalyzer can be used in combination with Standardized RT (StaRT)-PCR reagents from Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville, AL, USA) to obtain...

Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer

Duplications and deletions are known to cause a wide variety of human diseases. Many different methods are used for detecting such rearrangements, most based on either FISH or the quantitative analysis of PCR products. FI...

siRNA transfection optimization with the Agilent 2100 bioanalyzer

When working in the field of target validation, or when studying functional assays requiring the transfection of small interfering RNA (siRNA) into a cell, the presence of having a fast, easy-to-use method to optimize tra...

2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer

2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer The Agilent 2100 bioanalyzer is a unique, on-chip, electrophoresis and flow cytometry analy...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer and the Cell Fluorescence LabChip kit can be used to detect cell surface and intracellular protein targets by antibody staining. CD3 staining on Jurkat cell...

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer and the Cell Fluorescence LabChip kit can be used to determine the efficiency of transfection of mammalian cells using green fluorescent protein (GFP) as a ...

Biomatrica's RNAstable(TM) Stabilizes RNA at Room Temperature

...on than freezer storage. Furthermore, RNA samples recovered following stabilization in RNAstable can be used directly in applications such as qRT-PCR, bioanalyzer and microarray analysis, end-point PCR, gel analysis, cDNA synthesis and reverse transcription without inhibition or interference. "RNA is hard to w...

Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

...act RNA. Recent widespread use of the Agilent 2100 bioanalyzer has shown that this measure is in fact a very poor indicator of RNA quality. The bioanalyzer provides a rapid evaluation of RNA with small quan...s Quality assured using Agilent 2100 bioanalyzer Provided with RIN score and Agilent ...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

...1000 Spectrophotometer ◊ Agilent 2100 bioanalyzer ◊ Affymetrix GeneChip Scanner 3000 ... was assessed using the Agilent 2100 bioanalyzer (Figure 1). 6. 5 ng of total RNA from the...rial is also an important factor. The Agilent 2100 bioanalyzer plots shown inFigure2 are used to helpqualifythesa...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

...r, ranging from under 200 bases to over 600 bases in length (Figure 1B). The quality and quantity of aRNA after amplification were further examined by bioanalyzer (Agilent Technologies, Palo Alto, CA) (Figure 1C). Eight to ten micrograms (8-10 g) of aRNA was obtained from CapSure caps filled with microdissected ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...674; RiboAmp HS RNA Amplification Kit (Arcturus, Cat. #KIT0205) ◊ bioanalyzer 2100 (Agilent Technologies) ◊ NanoDrop ND-1000 Spectrophotometer ...cturers instructions. 3. aRNA quality was evaluated on the Agilent bioanalyzer 2100 using the Nanochip, and the aRNA yield was measured on the NanoDrop ND...

Microgenomic Expression Profiling

...t critical steps in the workflow using the Agilent bioanalyzer system. Final probe quantity, as well as efficienc...using the RNA 6000 PicoLabChip on the Agilent 2100 bioanalyzer (figure 1b). RNA concentrations < 2ng/L or 260/280... other cellular material. Furthermore, if the 2100 bioanalyzer output profiles do not exhibit clean 28S and 18S p...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...f the RNA was assessed and was found to be of high quality (Fig. 2). Peaks corresponding to the 28S and 18S ribosomal RNAs were clearly visible on the bioanalyzer profile (A) and pseudo-electropherogram (B). There was no detectable genomic DNA contamination, as evidenced by a lack of additional peaks to the righ...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

...tation generated by the protein 200 plus assay and bioanalyzer was less reproducible (CV of 19.0% and ...e data from the same samples run on the bioanalyzer system also yielded an average r 2 of ... a much higher intensity than those in the bioanalyzer electropherogram. The sensitivity of bot...

RNA & cDNA Purification Using RNAClean

...plications. Overall quality of RNA purified with RNAClean from an in vitro transcription reaction was assessed using the Agilent bioanalyzer 2100. Before testing, the RNA was incubated at either 4C for 2 days, at room temperature for 2 days, or 37C for 2 hours. No dete...

RNAPrep - Total RNA Isolation Purification

...cation RNAPrep was compared against two competitor kits for isolated total RNA quality. Analysis of samples by the Agilent 2100 bioanalyzer showed RNAPrep isolated RNA had consistently higher RIN scores (Figure 2) that asses 18/28S ribosomal peak highs and ratios alon...

RNAClean - RNA & cDNA in vitro Reaction Purification

...y. RNA was incubated at varying temperatures following RNAClean purification for up to 2 days and analyzed using an Agilent 2100 bioanalyzer to detect RNA degradation (Figure 3). There were no signs of degradation even after a 2 hour incubation at 37 C. ...

Unmask Blood RNA for Gene Expression Profiling

... was used to linearly amplify 2 g total RNA or RNA enriched by the GLOBINclear Kit. The resulting aRNA was resolved on an Agilent 2100 bioanalyzer RNA LabChip assay to monitor quality and quantity. Note the complete removal of the abundant "globin spike" in the GLOBINclear-t...

Total RNA from Whole Blood for Expression Profiling

... The remaining samples were stored at room temperature for three days before RNA isolation. RNA was quantified using Agilent 2100 bioanalyzer software after electrophoresis on an RNA LabChip Kit (Agilent). Values represent the average of three samples for both day 0 and...

SUPERIOR RNA FOR DEMANDING APPLICATIONS

...g for nucleases. The integrity and stability of each RNA is verified by capillary electrophoresis using the Agilent 2100 bioanalyzer as shown in Figure 2. The Purist Poly(A) RNA Available RNAqueous-Micro Kit (see sidebar) and the purified RNA was assessed on an Agilent bioanalyzer (Figure 2). This RNA was of sufficient quality and amount for real-time RT-PCR analysis, which showed consistency between replicates ...

RNA Amplification for Array Analysis

... shorter products. We recommend using an Agilent 2100 bioanalyzer to visualize these products. Amplification products ca...this data is still useful, it is less informative than bioanalyzer analysis. E. What is the typ...

Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

...treatment to fully eliminate RNA. Figure 2 shows an Agilent 2100 bioanalyzer scan of total nucleic acids, RNA, and DNA from an FFPE sample p...e RecoverAll Kit. Figure 2. Agilent 2100 bioanalyzer Scan Demonstrating Presence of RNA and DNA from FFPE Mouse Liver...

Ready for Real-time in 5 Minutes

... processed using the RNAqueous procedure is shown on the far right. The RNA samples were analyzed on an RNA LabChip using the Agilent 2100 bioanalyzer and show rRNA bands. Complete Kit for Cell Lysis and cDNA Synthesis The Cells-t...

Rapidly Synthesize Large Amounts of Capped RNA

...f capped RNA transcripts (Figure 1), mMESSAGE mMACHINE reactions also produce full-length transcripts. Figure 2 shows an Agilent bioanalyzer 2100 electropherogram of the 3.7 kb transcript. The presence of a single, strong peak indicates the predominance of full length ...

Protect RNA and Improve Target Cell Identification

...rom various types of tumor samples. (B) Representative Agilent bioanalyzer traces for RNA preparations from human colon cancer sections sho...(Cat# 1751) and used for subsequent microarray analysis. Agilent bioanalyzer results show that yield (mean SD, 142 12 g) and size distribut...

Hands-free Tissue Disruption No Homogenization Needed

...ass fiber filter purification step for Kit 1. (A) Agilent 2100 bioanalyzer scans demonstrate the high quality total RNA isolated using the ...tored at room temperature (2225C) for 6 days. (A) Agilent 2100 bioanalyzer gel of 28S and 18S rRNA bands for samples that were immediately ...

Got Small RNA?

...ame number of HeLa cells. Analysis of each fraction on a denaturing agarose gel, a denaturing acrylamide gel or the Agilent 2100 bioanalyzer showed that 28S, 18S, and 5.8 rRNAs were quantitatively recovered in the fraction depleted in small RNA. In contrast, RNAs small...

MessageAmp II: Use Less RNA for Array Analysis

...on is shown in Figure 2. Figure 3 shows bioanalyzer traces of aRNA produced after two round...NA Produced from Different RNA Inputs. bioanalyzer electropherogram of amplified RNA (sing... Derived From Different Total RNA Inputs. bioanalyzer electropherogram of amplified RNA deriv...

High Quality RNA Isolation Services

... protocols. Aliquots of the RNA were then stored at 4C for extended periods of time. 100 ng of the RNA was assessed on an Agilent 2100 bioanalyzer after (A) 1 month at 4C and (B) 7 months at 4C. Both scans indicate high quality RNA that is stable at suboptimal storage co...

Determinants of RNA Integrity and Purity

... alone (1-3). Figure 1 compares the Agilent 2100 bioanalyzer profiles of RNA isolated with a single-...n Total RNA Preparations. Agilent 2100 bioanalyzer electropherograms of RNA contaminated w... evaluate the baseline in the bioanalyzer electropherogram. In high quality RNA (rRNA ...

Improve RNA Isolation for Array Analysis and More

...iae RNA , C. albicans , and S. pombe RNA, isolated using the RiboPure-Yeast Kit and treated with DNase, was run on an Agilent bioanalyzer chip. The ratio of 28S:18S rRNA is given for each sample. Figure 2. RNA Yields From 3 Yeast Species Using...

Bacterial Whole Genome Array Analysis

...nome Arrays (Figure 3). Figure 1. Agilent bioanalyzer Image of E. coli mRNA Purified Using MICROB Express . 16S and ... control lane. Figure 2. Agilent bioanalyzer Electropherograms of mRNA from Figure 1. As seen in the (top) e...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

...er magnetic separation, the bacterial RNA remaining in the supernatant and can be precipitated with ethanol. Figure 1 shows an Agilent 2100 bioanalyzer electropherogram of a complex host-bacterial RNA population before and after bacterial RNA purification with MICROB Enrich . The...