Yield

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini

Recombinant Enterokinase for highly specific digestion of purifiedproteins or proteins in crude extracts Ed Marsh * Johanna Claycomb * ChaoFeng Zheng * Denise Wyborski* John C. Bauer * Joseph A. Sorge * A.K. Bernardino * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene introduces recombinant Enterokinase, an efficient, sitesp...

Trouble-shooting: Low yield

Here are some troubleshooting hints that we have gathered regarding low yield after PCR reactions: <a href="#Option1">Primer annealing temperature is too high...

Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System

Roche Applied Science pioneered the blending of thermostableDNA polymerases and the associated buffersystems. The well-known Expand High Fidelity andExpand Long Template PCR Systems were the first commerciallyavailable blends to provide amplification offragments up t...

ArrayScript RT: Maximize Yields of Full-length cDNA

Ambion scientists have engineered a mutant form of M-MLV reverse transcriptase to improve production of full-length cDNA. This engineered reverse transcriptase, <A href="http://www.ambion.com/catalog/CatNum.php?2048">ArrayScript (patent pending), produces significantly higher yields o...

Increase Protein Activity and Yield

Higher yields of protein than reticulocyte lysate systems lysate systems Easy to...

High Yield Transcription for Every Application

The MEGAscript Kit is the most frequently cited large-scale transcription kit, and with good reason. It enables the consistent transcription of high yields of full-length RNA (Figure 1). Ambion offers a complete line...

Maximize Viral RNA Yield from Biological Fluid

Ambions magnetic bead-based MagMAX Viral RNA Isolation Kit is optimized for isolating viral RNA from biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. RNA isolation from cell-free samples presents some unique challenges compared to RNA...

Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

RecoverAll Total Nucleic Acid Isolation Kit For decades, FFPE tissue has been the staple of histologists and pathologists for creating tissue slides and making patient diagnoses. More than 400 million FFPE tissue samples exist for cancer studies alone, and many of...

Synthesize High Yields of Biotinylated aRNA

Ambion now offers an improved MessageAmp II Kit with biotin-11-UTP. MessageAmp II-Biotin Enhanced provides a complete single round amplification kit for biotin array analysis. The biotin modified UTP and ratio of labeled...

Translate High Yields of Active Protein

Translation products have higher biological activity than those from other E. coli Efficient synthesis of a wide range of prokaryotic and eukaryotic proteins Fast translation and hi...

Translate High Yields of Biologically Active Proteins

Cell-free translation systems are a convenient method for synthesizing proteins in vitro. The major advantages of these in vitro translation systems over conventional in vivo systems are greater flexibility, faster reaction times, and less...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

Kathleen A. Maguire-Zeiss, Brandon K. Harv e y, Rita E. Giuliano andH o w a rd J. Federo ff, University of Rochester, Rochester, NY, USA Transfection efficiency is dependent upon a number of factors, including method of transfection, cell type, and DNA concentration and purity. Liposome-mediated transfect...

DNA Yield with Oragene

DNA Genotek, Ottawa, Ontario, Canada Oragene gives higher DNA yields than other oral collection methods. The median yield of DNA from Oragene samples is 110 g. In comparison, buccal swabs may yield as little as 1.9 g of DNA. The number...

Food science: increasing crop yields

Protein-containing plants are staples in the diets of all countries. The ability to produce enough crops is essential to the well-being of all citizens, for people have to have enough to eat before they can advance to higher endeavors. <P>Uncontrollable changes in weather such as the amount of rainfall, the occurrence of fro...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

Over the last several decades, geneticists have learned how to manipulate DNA to identify,excise, move and place genes into a variety of organisms that are quite different genetically fromthe source organism. A major use for many of these recombinant organisms is to produceprot...

A technique for increasing yields in bioreactors and disposable cell-culture systems

FibraCel disks are a new cell-growth matrix that dramatically increase yields in packed-bed reactors, andhave also proven highly effective when used in spinners, disposable bottles and bags. New BrunswickScientific offers two new devices that use FibraCel disks to increase productivity: the FibraStage , a simpleand disposable c...

Lucigen receives $750K grant to boost biofuel yields

The product is the company's second bioprospecting find as the result of d...

The science is clear: Ethanol yields a net increase in energy

The final report by the Governors Consortium on Bio-Based Industry, issued Friday, will no doubt spark another round of anti-ethano...