Yeast

Epitope-Tagging Vectors for Functional Analysis in Yeast

Stratagene Stratagene has developed a series of epitope-tagging vectors to expressand functionally analyze eukaryotic genes in the budding yeast Saccharomyce...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

Stratagene Cloning Systems, Inc. The ESP LIC cloning and expression system features rapid and high-yieldproduction of recombinant proteins in yeast. This system usesligation-independent cloning (LIC) for efficient and dire...

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

Electroporation of Yeast Artificial Chromosomes

Maren Bell and Robert Mortimer, Human Genome Center, Divisionof Cell and Molecular Biology, Lawrence Berkeley Laboratory,Berkeley, California 94720 Transformation of yeast with yeast artificial chromosomes (YACs) has traditionally been performed by a PEG-spheroplast procedure. However, the...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse Department of Biochemistry and Molecular Biology, Monash University,...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse Department of Biochemistry and Molecular Biol...

New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

, an automated device for counting mammalian cells, the new NucleoCounter YC-100 for yeast combines a fluorescence microscope with CCD camera and integrated i...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

Over the last several decades, geneticists have learned how to manipulate DNA to identify,excise, move and place genes into a variety of organisms that are quite different genetically fromthe source organism. A major use for many of these recombinant organisms is to produceprot...