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Epitope-Tagging Vectors for Functional Analysis in Yeast

Stratagene Stratagene has developed a series of epitope-tagging vectors to expressand functionally analyze eukaryotic genes in the budding yeast Saccharomyce...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

Stratagene Cloning Systems, Inc. The ESP LIC cloning and expression system features rapid and high-yieldproduction of recombinant proteins in yeast. This system usesligation-independent cloning (LIC) for efficient and dire...

Yeast Protein Production System Features High Yields and One-Step Purification

The ESP system provides high-yield protein production in a eukaryoticorganism Quinn Lu * Bruce Jerpseth * Tim Sanchez * John C. Bauer * AlanGreener Stratagene Cloning Systems, Inc. provides novel opportunities for rapid, inexpensive and high-yield p...

Biotech industry reaches midpoint in 50-year maturation cycle

An interesting article in ) on March 1 states unequivocally that most industry cycles run about 50 years until they mature. If this...

Electroporation of Yeast Artificial Chromosomes

Maren Bell and Robert Mortimer, Human Genome Center, Divisionof Cell and Molecular Biology, Lawrence Berkeley Laboratory,Berkeley, California 94720 Transformation of yeast with yeast artificial chromosomes (YACs) has traditionally been performed by a PEG-spheroplast procedure. However, the...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse Department of Biochemistry and Molecular Biology, Monash University,...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse Department of Biochemistry and Molecular Biol...

New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

, an automated device for counting mammalian cells, the new NucleoCounter YC-100 for yeast combines a fluorescence microscope with CCD camera and integrated i...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

Over the last several decades, geneticists have learned how to manipulate DNA to identify,excise, move and place genes into a variety of organisms that are quite different genetically fromthe source organism. A major use for many of these recombinant organisms is to produceprot...

Reader predictions would make 2007 quite a year

From Virtual worlds get r...

2006: A great biotech financing year, unless you were going public!

Well folks, this is my last article of the year, and I have talked less about music this year than in years past, so my New Year's resolution for this column is bring back more music (hopefully something blue, rock, or folk-related). Part of the problem is that I simply have not gone to as many concerts this past year, or bought many new CDs (something else I will n...

Presto! A 94-year-old woman goes online

Such was the case this past weekend, when I installed...

Wait `till next year OK for baseball, not broadband

There should be as m...

Fact and friction: Putting election-year stem cell claims under the microscope

Or perhaps it only seems so in light of 2006 campaign year claims and counter-claims swirling around the "wedge issue" of human embryonic stem cell research. To assist those who won't complete...

UW System says goodbye to Lawson after 5 years, $26 million

payroll and benefit softwar...

TDS restates years of revenues, shows slight profit drop

Phone and data company TDS, also the corporate parent of U.S. Cellular, said its revenues for the last quarter were $1 billion, an increase over results from last year that it had to restate due to accounting problems. Net income for the quarter was $41.5 million, down from $43.3 million in the same quarter a year ago. TDS said in November 2005 it would have to restate some resu...

CEO of TDS Telecom to retire at end of year

Barr's replacement is already on hand: David Wittwer, 44, most recently president of TDS Telecom's local telecommunications operations, has been named chief operating officer and will step up as president and C...

Wisconsin Virtual Academy holds info sessions for new year

Wisconsin Virtual Academy, an online K-8 public school available statewide, is holding information sessions for parents until the end of the state's open enrollment program on February 24. Children enrolled in the academy are lent a computer, monitor, printer and access to the online curriculum. They work at home with a parent or other responsible adult and correspond with public school...

Sonic Foundry expects break-even this year

A company statement said revenue is expected to pick up later this year and projected cashflow break-even sometime in the next three quarters. For now, losses expanded slightly...

State tracks more angel investment, at least $19M last year

The investor tax credits offered through Act 255 have let Wisconsin, for the first time, start to officially track the volume of investments by individuals. "Even though it's not entirely complete, it's the first good pictu...

UW-Madison MBA ranks 51st in U.S., from no ranking last year

The These rankings are based on alumni careers and salary, diversity, and research capabilities. The Financial Times compiled data p...

2005 was a tough year for U.S. biotech financing

Another research tool I have always found useful for this analysis is 's financial tracking information on the biotech industry. O...

Biomedical alliance marks first full year

Founded in 2004, the alliance received $500,000 from the state, which was matched by participating academic institutions. That was not qu...

The year of working together (really!)

I share this with a minor tremor of trepidation. I worked in IT publishing through the late '80s, when each year was declared w...

2005: the year of the comeback

The last column of the year is an invitation for reflection, and this one is no different. These last 12 months have seen a resurgence of the technology market and well no doubt look back on 2005 and pronounce it the year the Internet economy got back on its feet. In the overall scheme of things, the previous four years were a standing eight count, not the death blo...

Space port could open in Sheboygan in next 10 years

Private space flights could be launched from Wisconsin in the next ten years, according to a proposal that has passed the Joint Finance Committee 14-1 and could reach the Senate for a vote this month. State Senator Joe Leibham, R-Sheboygan, has a vision of a private spaceport in Wisconsin that would be regulated similarly to airports. Leibhams bill, SB 352, would establish a Wisconsin Ae...

Passports to contain RFID chips next year

International travelers will find a new accessory among their standard travel gear starting in late 2006: an RFID-equipped passport. The new passports, mandated by State Department regulations, will feature a 64 KB chip containing a duplicate of the information featured on the passport's physical page, which the government hopes will strengthen national security. "The electronic...

Internet Scout celebrates 10 years on web, eyes new project launch

From the beginning, the Internet Scout Project has been on the job, providing better tools for finding, filtering and presenting online information. Internet Scout is currently celebrating its first decade on the Web and also looking forward to a fu...

Food microbiology symposium will mark 25 years

The Food Microbiology Symposium is Oct. 15-19 and features speakers from academia, industry and regulatory agencies will provide an overview on various topics including: GMO...

Farm Aid celebrates 20-year journey, heads back to Illinois roots

As indicated by the in an article this weekend on this unique phenomenon, the American farm is not on...

UW engineering career fair sees highest attendance in four years

Sponsored by , the fair brought at least 200 companies...

Wisconsin ranks low in mid-year health care capital investment report

The report was compiled by , a business formation and management counseling service specializing i...

ITEC coming to Milwaukee this year

The conference will feature <a href="http://www.microsoft.com" target="b...

Kanavas thinks personal ID theft bill will pass this year

State Senator Ted Kanavas remains optimistic that his bill requiring notification of electronic data breaches will pass the legislature yet this year. Senate Bill 164, co-authored by Kanavas, R-Brookfield, and Representative Jeff Fitzgerald, R-Horicon, would require companies that keep personal data on customers and clients to notify those people when that data is obtained by unauthoriz...

Star panel re-evaluates Sarbanes-Oxley one year in at SEC headquarters

The event was a roundtable of public companies, auditors, investors, and members of the legal community to discuss the pros an...

Northwoods Software co-owner distills 30 years of business experience

Early in his management career, Bob Weisenberg read a lot of books on management and leadership. One day, when he was about one-third of the way through yet another book, he realized he had read it before. Now retired after a 30-year career in the software industry, Weisenberg says the way to excel at leadership is by getting good at applying a very limited number of skills, not by readi...

2004 a banner year for biotech financing

does a great job of tracking U.S. biotech financing in all its shapes and forms (including funds flowing through due to partnerships with other biotech companies and Big Pharma). From its recent analysis, it would appear that 2004 was a very good year for biotech in almost all the financing categories. Lets take a look:<br...

Year ahead offers payoff for hard work

First-of-year columns are ripe for prognostication and this one is no different. Like a creaky car on an old wooden roller coaster, the technology industry has been laboring up, up, up the steep incline of recovery. After so much work, the year ahead will offer a bit of payback, a little joy ride. And to be clear in my metaphor, lets be sure to picture a few playfu...

Ten resolutions that could produce a happy new (tech) year

Wisconsins real-time economy kept pace with a globally paced clock in 2004 as the state became better known as a place where innovation, intellectual property and investment are welcome. Dont pop the Champagne corks just yet: Wisconsin still has a long way to go before its economy is creating as many high-wage jobs as the state lost in the three-year manufacturing recession. The chasm be...

Sonic Foundry triples revenue, looks for profit next year

That leaves the company with an overall loss this year of $5.4 million. Last year, operational losses were greater but the $11.9 million sale of d...