Vitro

GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip expression arrays. The GeneChip Sample Cleanup Module was developed as part of an on...

In-Vitro Metabolism Studies Using Data-Dependent LC/MSn

P.R. Tiller, Thermo Finnigan W.H. Schaefer, D.M. Murphy, SmithKline Beecham Pharmaceuticals Metabolite Characterization The data presented here can be acquired using the Thermo Finnigan LCQ Series of ion trap mass spectrometers....

RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

Gene arrays can be used to simultaneously measure the expression levels of thousands of genes in a single experiment. One limitation of this technology is that large amounts of RNA may be required for each array hybridization experiment -- typically 10 g of total RNA. This becomes problematic when working with limited sampl...

RNAClean - RNA & cDNA in vitro Reaction Purification

Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates...

High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

A variety of siRNA applications, including siRNA library screening, require the efficient delivery of hundreds to thousands of siRNAs to cells. Most published siRNA studies describe performing transfections in 6 well and 24 well formats. Ambion has developed two reagents, siPORT...

Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs

siRNA Construction Kit uses an in vitro transcription reaction and column purification to prepare transfection-ready siRNAs. With this kit, multiple siRNAs can be prepared in ~24 hours. Below is one example of how siRNAs prepared by the Silenc...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. Here, resear...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

Micronucleus assays • toxicology • image analysis • IN Cell Analyzer Micronucleus induction is a key characteristic of genotoxic compounds and analysis of micronuclei formation resulting from DNA strand breakage (clastogens) or interference with chromosome segregation (aneugens) is an important component of toxic...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

SmithKline Beecham Pharmaceuticals, UK FlashPlate is a 96-well white polystyrene microplate with scintillant-coated wells designed for highvolume, in-plate radiobinding assays. This study investigates the use of FlashPlate as a platform for the measurement of G-protein act...

Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor

Dpartement dImmunologie, Laboratoires Fournier S.A., France TGF- type I and type II receptors form ligand dependent heteromeric signaling complexes, in which transforming growth factor- receptor type II (TRII) tends to act as the primary receptor....

In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

Abnormalities in neurotransmission within the brain can lead to several disease states, including anxiety, depression or schizophrenia. Extensive research has been carried out to discover the mode of action of various transmitters, in order to understand and combat these diseases. In many cells, binding of the neurotransmitter to its recept...

Invitrogen boosts drug development capability

The new capability will be made possible by 's recent acquisition of Sentigen Holding Corp., which will be integrated with Inv...

Prodesse joins forces with Invitrogen

As part of a new license agreement, Prodesse will add to its offerings the first diagnostic assays with light upon extension (LUX) techn...

The world of in-vitro diagnostics is another Midwest success story

I am in a real quandary this week: I have a number of juicy topics I wanted to review with you, including my recent participation in the annual Israel biotech/medical device conference (BIOMED 2006), recent participation in meetings about India and my growing insights into the Indian biotech market, happenings in Japan, and other research that has crossed my transom...

The world of in vitro diagnostics is another Midwest success story

I am in a real quandary this week: I have a number of juicy topics I wanted to review with you, including my recent participation in the annual Israel biotech/medical device conference (BIOMED 2006), recent participation in meetings about Indian and my growing insights into the Indian biotech market, happenings in Japan, and other research that has crossed my transo...

Invitrogen to buy Norwegian biotech with operations in Brown Deer

Dynal Biotech ASA</...