VIT

Improved Sensitivity for Staining RNA with SYBR Gold Stain

II still video system provides quality images fromnanogram quantities of RNA Genina Troutman Ian Miller Jeff Mueller MikeKobrin Stratagene Gold nucleic acid gel stain(Molecular Probes), combined with Stratagenes Eagle Eye II stillvideo system, allows t...

cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR

RT-PCR has become an essential method that allows researchers to determine the presence, structure and level of expression of an mRNA transcript in the organism, tissue or cell of interest. The basic protocol involves the synthesis of a cDNA copy of all the mRNA present in the s...

GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip expression arrays. The GeneChip Sample Cleanup Module was developed as part of an on...

Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer

Mark Churchill , John Henninge, 1 Thermo Finnigan, Stafford House, 1 Boundary Park, Boundary Way, Hemel Hempstead, HP2 7GE, UK; 2 Aker University Hospital, Hormone Laboratory, Section for Doping Analysis,Trondheimsv...

Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology

RohanA.Thakur, AndrewW. Guzzetta, and Julie A.Horner The data presented here can be acquired using a Thermo Finnigan LCQ Advantage, LCQ Deca XP, and Deca XP Plus ion trap mass spectrometer....

In-Vitro Metabolism Studies Using Data-Dependent LC/MSn

P.R. Tiller, Thermo Finnigan W.H. Schaefer, D.M. Murphy, SmithKline Beecham Pharmaceuticals Metabolite Characterization The data presented here can be acquired using the Thermo Finnigan LCQ Series of ion trap mass spectrometers....

RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

Gene arrays can be used to simultaneously measure the expression levels of thousands of genes in a single experiment. One limitation of this technology is that large amounts of RNA may be required for each array hybridization experiment -- typically 10 g of total RNA. This becomes problematic when working with limited sampl...

RNAClean - RNA & cDNA in vitro Reaction Purification

Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates...

High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Immunoassays have traditionally employed colorimetric indicators. As such, multiple dilutions of samples were necessary to ensure that they fell within the dynamic range of the assay. Recently, chemiluminescent immunoassays, with gre...

Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is a new recombinantreverse transcriptase (RT) expressed in E. coli. Theenzyme has RNA-directed DNA polymerase activity,DNA-dependent DNA polymerase activity, unwindingactivity, and, importantly, RNase H activity....

Increased Sensitivity in Microarray Analysis

Transcriptor Reverse Transcriptase inthe Eberwine Target Preparation Workflow Reproducibility and sensitivity are key parameters for thequality of a gene expression profiling experiment. Theimproved reliability of commercially avail...

HIGH SENSITIVITY qRT-PCR

More sensitive than SuperScript II and other RNase H Linear quantitation of mRNA targets over a 106-fold range Quantitate transcripts from as little as 500 fg total RNA 1/2...

Increase Protein Activity and Yield

Higher yields of protein than reticulocyte lysate systems lysate systems Easy to...

High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

A variety of siRNA applications, including siRNA library screening, require the efficient delivery of hundreds to thousands of siRNAs to cells. Most published siRNA studies describe performing transfections in 6 well and 24 well formats. Ambion has developed two reagents, siPORT...

Measuring RNase Activity A Real-time Kinetic Analysis

The RNaseAlert QC System, when used in conjunction with a fluorometer capable of kinetic analysis, provides a rapid and ultra-sensitive assay for determining RNase A specific activity. The RNaseAlert QC System also detects a variety of other nucleases, including RNase A, T1, RNase I, micrococcal nuclease, S1 nuclease,...

Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells

More than 200 miRNAs have been cloned and sequenced from human and mouse samples. Each miRNA apparently regulates multiple genes, and miRNAs are thought to regulate as many as 10% of human genes [1]. Several reports predict mR...

RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control

RNase activity varies dramatically across different tissues. A comparison of total RNase activities for 8 different mouse tissues showed that total RNase activity spans a 181,000-fold range from pancreas to brain (Figure 1), which highlights the level of RNase control required for succ...

Translate More Protein with Higher Biological Activity

Fully compatible with eukaryotic protein translation Better yields and faster translation than traditional reticulocyte lysate systems Higher biological activit...

Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs

siRNA Construction Kit uses an in vitro transcription reaction and column purification to prepare transfection-ready siRNAs. With this kit, multiple siRNAs can be prepared in ~24 hours. Below is one example of how siRNAs prepared by the Silenc...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. Here, resear...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

Andreas Henke and Eunike Grohmann, Institute of Virology, Medical Center,Friedrich Schiller University Jena, Winzerlaer Str. 10, 07745 Jena, Germany The Helios gene gun-mediated DNA transfer can be usedas a fast and efficient method to transfect living cells. Inthis study we describe the preparation of DNA-coatedmicroc...

New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

A. Bergh, M. Carlsson, A. Karlsson, and H. Lindgren GE Healthcare, Uppsala, Sweden Histidine-tagged proteins* can be quickly and simply purified on His GraviTrap columns prepacked with Ni Sepharose 6 Fast Flow without the need for a pump or purification system. The protein binding capacity (approx. 40 mg/column) is among the hi...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

Micronucleus assays • toxicology • image analysis • IN Cell Analyzer Micronucleus induction is a key characteristic of genotoxic compounds and analysis of micronuclei formation resulting from DNA strand breakage (clastogens) or interference with chromosome segregation (aneugens) is an important component of toxic...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

<P >J. Turner GE Healthcare, The Maynard Centre, Cardiff, UK Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ Kinase Assay Kits gene...

Long-Term Instrument Control and Connectivity Solutions

The key to developing long-term instrument control applications is flexible and scalable software and hardware that enable seamless connectivity to any instrument in your application and offer built-in capabilities for advanced data analysis and presentation. Your choice of instrument should not limit or control your software choice, and your software preference...

Increase Your Organizations Productivity with LabVIEW

This paper addresses managers in different industries and describes the benefits of implementing their applications in LabVIEW. It describes how LabVIEW increases productivity, and touches upon subjects such as ease of use, complete functionality, available tools and resources, and performance. This paper also describes the main criteria managers should consider when choosing th...

High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS

The animal meat samples were prepared by homogenization, organic solvent washes, hydrolysis and derivatization follo...

Femtogram Sensitivity of the Antipsychotic Drugs Raclopride and Ritanserin Using a Varian 1200L LC/MS/MS

Varian, Inc. These two compounds were used to demonstrate low-level sensitivity of...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

SmithKline Beecham Pharmaceuticals, UK FlashPlate is a 96-well white polystyrene microplate with scintillant-coated wells designed for highvolume, in-plate radiobinding assays. This study investigates the use of FlashPlate as a platform for the measurement of G-protein act...

Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor

Dpartement dImmunologie, Laboratoires Fournier S.A., France TGF- type I and type II receptors form ligand dependent heteromeric signaling complexes, in which transforming growth factor- receptor type II (TRII) tends to act as the primary receptor....

In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

Abnormalities in neurotransmission within the brain can lead to several disease states, including anxiety, depression or schizophrenia. Extensive research has been carried out to discover the mode of action of various transmitters, in order to understand and combat these diseases. In many cells, binding of the neurotransmitter to its recept...

Maintaining High Sensitivity when Working with Small-Volume Samples

20 L, 50 L, 250 L, or 1 mL <P > <br >enzymes amino acids <P > <br>photon-counting <P >Samples can be valuable. Either you dont want to waste them, or you may not have very much with which to start. Biological prot...

Kinetic determination of vitamin B1

) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase...

Wideband sensitivity of the FluoroLog-3

Many companies claim high sensitivity for their spectrofluorometers. In their specifications, instrument vendors often cite excitation of de-ionized water at 350 nm, which results in Raman emission at 397 nm (an energy loss of 3380 cm ). This Raman-emission band of water is used, because the signal is red-shifted and weak...

Fluorescence assay for determination of protease activity

VICTOR is a new multitechnology and multilabel counter from Wallac that can me...

Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

such as for struct...

Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery

Department of Drug Metabolism and Pharmacokinetics, Sch...

Trace Analysis Optimizing Sensitivity Finnigan Surveyor PDA Detector

1 Astra Zeneca R&D, Charnwood,UK</...

Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns

While full coverage C18 phases have been responsible for the growth of HPLC as a reliable high efficiency technique, it has become apparent in recent years that such column types are not suited for reliable analysis of highly polar compounds under low organic or aqueous mobile phase conditions...

Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins

With the introduction of two new reversed-phase column types, TSKgel ODS-100V and TSKgel ODS-100Z, excellent chromatographic performance and selectivity can be achieved for a wide range of sample types. TSKgel ODS-100Z and TSKgel ODS-100V are based on a spherical, five micron, silic...