Trop

A Versatile Power Supply for All Electrophoresis Applications

Douglas Drake Michelle Cayouette Lisa Filippone Bob LeSchofs Stratagene 4000 Power Supply Stratagene has developed the new SmartPower...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as quickly as possible are adapted from Eppendorf Brochure...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Optimizing electroporation parameters

To ensure that maximum transfection rates are achieved, the electroporation parameters should be optimized for each new cell line. The guidelines listed below for determining the ideal electroporation parameters as simply and as quickly as possible are adapted from Eppendorf Brochure...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompar...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocomp...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompar...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

Optimizing electroporation parameters

Other important parameters for optimizing electroporation. Field strength Pulse length <a href="http://www.biocompa...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

Michael Hepburn and Glenn A. Miller, Ph.D., Molecular Profiling Laboratory, Genzyme, Framingham, MA The search for sequence variations in genomic DNA is important in the study of genes that play a role in the development of cancer and a variety of other single and multiple gene disorders...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

K. Sestak, L. Kim, K.-O. Chang and L. J. Saif, Food Animal Health Research Program, Ohio Agricultural Research and Development Program, The Ohio State University, Wooster, OH 44691 Three major structural proteins of the porcine coronavirus, transmissible gastroenteritis virus (TGEV), membrane (M), nu...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

Martin A.O.H. Menke*, Axel Reinecke-Lthge*, Barbara Mllmann*, Angela Kellner, Jutta Lttges*, Gnter Klppel* * Department of Pathology and Department of Hematopathology, Christian- Albrechts University Kiel, Michaelisstrae 11, D-24105 Kiel, Germany. Introduction Genetic alterations/variations are...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

Terry H. Landowski, Ibrahim Buyuksal, and William S. DaltonH. Lee Moffitt Cancer Center and Research Institute, University of SouthFlorida, 12902 Magnolia Drive, Tampa FL 33612 Introduction Identifying of mutations within specific genes has become an important strategy in determining the diagnosis and prognosis f...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

Kristi M. Miller, Tobi J. Ming and Ruth E. Withler, Pacific Biological Station,Department of Fisheries and Oceans, Nanaimo, B.C. V9R 5K6, Canada Introduction We describe a rapid and sensitive method for the detection of nucleotide sequence variation which can be used for largescale screening of population...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

Tochigi Cancer Center, Tochigi, Japan Introduction Denaturing gradient gel electrophoresis (DGGE) is one of the most consistently used mutation scanning methods. It has evolved enormously over the last decade to be widely used particularly in gene diagnostic l...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

, and Jef Raus , 1 Dr. L. Willems-Instituut and 2 Limburgs Universitair Centrum, Universitaire Campus, B-3590 Diepenbeek, Belgium Introduction Phenylketonuria (PKU) is a hereditary disease that gives rise to elevated blood leve...

Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

Bernd Eschweiler and Beate Kilb, Institute for Water Research, Schwerte, Germany Introduction Bacterial diversity in environmental samples is usually determined by a characterization of isolated strains. A problem for the analysis and characterization of microbial communities is the inability to culture...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

Most spectrophotometric methods for inorganic phosphorus (Pi) analysis are based on the color formed upon the reduction of a phosphomolybdate complex. The classic (and still widely-used) method is that of Fiske and Subbarow , in which the reduction is carried out with sulfite and aminonaphtholsulfonic acid. Over the years, modi...

UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)

Molecular Devices Corporation. 7/99 In 1994, Molecular Devices introduced SPECTRAmax , the first microplate reader equipped with a monochromator, rather than bandpass filters, for wavelength selection. It was also the first microplate reader capable of measuring absorbance below 340 nm. These new features transformed the micropl...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

(Research Associate II, Ambion, Inc.) Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells are often desired for such experiments because they are mor...

Optimizing Chemical Transfection and Electroporation of siRNAs

(Associate Scientist, Ambion, Inc.) (Vice President and CSO, Cyto Pulse Sciences, Inc.) Low transfection efficiency is the most frequent cause of unsuccessful gene silencing...

High Throughput siRNA Electroporation

Efficient siRNA delivery with minimal cell mortality Electroporate up to 96 samples at the same time Deliver siRNA into primary, hard-to-transfect, and suspension cells D...

Oligonucleotide Purity Analysis by Capillary Electrophoresis

Introduction Millions of oligonucleotides are synthesized each year for use as primers or probes in genetic analysis. Quality control of these preparations has traditionally been done by slab gel electrophoresis or HPLC, but these methods may require expensive reagents and laborious protocols, and may...

Preparative Nondenaturing Gel Electrophoresis Used in the Purification of an Esterase Involved in Insecticide Resistance, Rev C

Dr Albert J Ketterman and SHP Parakrama Karunaratne, Dept. of MedicalParasitology, London School of Hygiene and Tropical Medicine, Keppel St,London WC1E 7HT UK Introduction In attempts to control Culex quinquefasciatus as a worldwide vector of diseases such and filariasis and encephalitis, mosquito field populati...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

Contributed by Philip W Miller, Waltraud I Dunn, and Robert R Schmidt, University of Florida, Dept. Microbiology and Cell Science, Gainesville, FL 32611 USA Florida Agricultural Experiment Station Serial Number T-00256 Introduction One of the research goals of this laboratory is to elucidate t...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

Udo Reischl and Christian Gerdes, Institute of Medical Microbiology and Hygiene,University of Regensburg, 93053 Regensburg, Germany Abstract Serodiagnosis of Epstein-Barr virus (EBV) infection is currently based on the detection of antibodies to distinct EBV antigens. In the past few years the specificity...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

K. H. Valkonen*, N. Marttinen*, H.-L. Malinen*, V.-P. Jaakola** and T. Alatossava* * Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland ** Department of Biological and Environmental Science, University of Jyvskyl, PL 35, FIN-40351 Jyvskyl, Finland...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

Menu B. Leddy, Biotechnology Research Department,Orange County Water District, 10500 Ellis Ave., Fountain Valley, CA 92708 Identification and characterization of bacterial species from membrane biofilms has been a microbiological challenge. A vast majority (>90%) of bacterial cells from membrane...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

M. E. Eberle, Wisconsin Regional Primate Research Center, University of Wisconsin, Universi...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

Bio-Rad Laboratories, Paul Zoller, Theresa Redila-Flores, Dan Chu, andAsmita Patel, Ph.D. As the number of diseases and disorders linked to gene mutations grow, the need for scanning single-base mutations becomes ever more vital to extending understanding of disease-risk predictor mutations and their...

DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

The Trans-Blot SD DNA/RNA agarose gel support frame, used with the Trans-Blot SD semi-dry electrophoretic transfer cell, allows blotting of DNA/RNA from agarose gels to nylon membrane in minutes, without any gel pretreatments. The Trans- Blot SD semi-dry electrophoretic transfer cell generates a high fiel...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

Marie Nguyen and William Strong, Bio-Rad Laboratories, Inc.,6000 James Watson Drive, Hercules, CA 94547 USA Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an established technique employed for protein separation. However, this traditional gel-based method involves...

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

Contributed by Alan T. Bakalinsky Department of Food Science and Technology, Oregon State University, Corvallis, OR 97331-6602 Chromosomal profiles of wine strains of Saccharomyces cerevisiae were compared to those of a standard laboratory karyotype. Qualitative analysis supported previous genetic...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

, and Scott E. LaPatra 1 Department of Fish and Wildlife Resources, University of Idaho, Moscow, ID USA 2 Clear Springs Foods Inc., Research Division, PO Box 712, Buhl, ID USA Coldwater disease (CW...

Electroporation of Primary Bone Marrow Cells

, and Lawrence B. Schook, Laboratory of Molecular Immunology, Department of Animal Sciences, University of Illinois, Urbana- Champaign The transfer of genes into eukaryotic cells permits the study of many things, such as gene regulation, gene structure, and gen...

Electroporation of Yeast Artificial Chromosomes

Maren Bell and Robert Mortimer, Human Genome Center, Divisionof Cell and Molecular Biology, Lawrence Berkeley Laboratory,Berkeley, California 94720 Transformation of yeast with yeast artificial chromosomes (YACs) has traditionally been performed by a PEG-spheroplast procedure. However, the...