Tri

Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue

In situ Adapter for the RoboCycler 40 Temperature Cycler allows rapidamplification of rare mRNA Department of Medicine, University of Illinois at Chicago Figure 1: RoboCycler 40 Temperature Cycler In Situ Adapter Syst...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

Stratagene A 28-base insertion was introduced into a plasmid vector using theQuikChange site-directed mutagenesis kit.* Theinsertion created three unique restriction enzyme digestion sites. Plasmid DNAisolated from 9 o...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

Stratagene Restriction endonucleases are used in many molecular biology protocols,making the functional purity of critical importance. Each restriction enzymepreparation must be free of contaminants that can potentiall...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

Gel Cleanup Kit George Halley, Eppendorf 5 Prime, Inc. Agarose gel electrophoresis is used extensively to separate and analyze DNA that is subsequently used for downstream applic...

TripleMaster PCR System

3.1 <a href="#c...

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Phase Lock Gel may be used in conjunction with TRIzol Reagent for the isolation of total RNA from cell and tissue samples. Increased yields are observed using this method, because the entire aqueous phase can be recovered without interphase contamination. Below is a protocol outlining the steps involved in RNA...

Principles and applications of electrical cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cance...

TripleMaster PCR System

3.1 <a href="#c...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Phase Lock Gel may be used in conjunction with TRIzol Reagent for the isolation of total RNA from cell and tissue samples. Increased yields are observed using this method, because the entire aqueous phase can be recovered without interphase contamination. Below is a protocol outlining the steps involved in RNA...

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany The classic RNA isolation procedures are...

Clostridium botulinum

Clostridium botulinum...

Clostridium botulinum

Clostridium botulinum...

The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments (100 bp to roughly 2,000 bp). However, demanding...

TripleMaster PCR System: Direct Comparison with Competitors

Eppendorf 5 Prime, Inc., Boulder, CO, USA This experiment was performed to compare the Eppendorf TripleMaster PCR System with the long-range PCR products of leading competitors. Eppendorf was compared to competitors T...

TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates

to Amplify Long gDNA Templates Eppendorf 5 Prime, Boulder, CO, USA Eppendorf provides a complete system for long-range PCR1 analysis of gDNA. The Perfect gDNA Bloo...

TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

Eppendorf 5 Prime, Boulder, CO, USA PCR* technology has been widely used in molecular biology research, providing scientists with an efficient tool for studies such as genome analysis, sequenci...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

Eppendorf 5 Prime, Boulder, CO, USA The polymerase chain reaction (PCR*) is a powerful and sensitive technique used by scientists to amplify large quantities of DNA. Although the reaction mix and thermal cycling conditi...

Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer

Nicola Hughes, Jean-Jacques Dunyach, 1Biovail Contract Research,Toronto,Ontario,Canada; 2Thermo Finnigan,San Jose,CA,USA; 3Therm...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...

Wisconsin tries to woo another stem cell company

The company, spun out of the in Athens in 2003, has received funding commi...

WARF will ease stem cell licensing restrictions

are a step in the right direction, but don't go far enough to r...

Third Wave lands European distribution deal, reports high earnings

MADISON announced Tuesday a four-year marketing and distribution agreement. Under the terms of the agreement, Third Wave has granted Belgium-based Innogenetics the exclusive European marketing and distribution rights for select Invader molecu...

Merge eFilm Builds Distribution Channel

MILWAUKEE, WI - - has signed agreements with four European companies to resell or distribute Merge eFilm's FUSION RIS/PACS and eFilm Workstation solutions. These full-service healthcare solution firms will increase the availability of Merge eFilm's products and services throughout European healthcare organisat...

Japan, Midwest Biotechnology Industry Leaders Identify Steps for Greater Collaboration Between Countries

CHICAGO,IL--Top industry, government and academic leaders in the biotech and life science industries from the Midwest U.S. and Japan gathered Wednesday in Chicago as part of the JETRO Midwest-Japan Biotechnology Summit. The topics covered similarity of challenges faced by Midwest Biotech companies and their counterparts in Japan. The goal was to foster discussion and lay down specific p...

The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling

In general, oligonucleotide microarrays are composed ofa two- or three-dimensional solid surface where DNA,used as a capture molecule (probe), is attached in anorderly fashion. Each of the oligonucleotide probes onthe microarray corresponds to a specific target gene,...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...

Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining

A fast and accurate method to detect green fluorescent proteinexpression in samples of low cell numbers This Application Noteaddresses the major problemsthat many researchers workingwith primary cells have, for example,limited availability, quan...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

K. H. Valkonen*, N. Marttinen*, H.-L. Malinen*, V.-P. Jaakola** and T. Alatossava* * Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo, Finland ** Department of Biological and Environmental Science, University of Jyvskyl, PL 35, FIN-40351 Jyvskyl, Finland...

InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C

Prepare DNA from blood, cultured cells, or bacteria: Just incubate sample with InstaGene matrix at 56C for 1530 min, then boil for 8 min. InstaGene matrix absorbs cell lysis products, producing an improved substrate for PCR amplification. Use whol...

Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix

Paul Burr, Wellcome Clinical Scholar, Department of VeterinaryPathology, University of Glasgow, Bearsden Rd, Glasgow. G61 1QH. A method that can be conveniently used to prepare DNA from a wide range of tissues for analysis in the Polymerase Chain Reaction is described. The method is both quick...

Enzyme Purification With the Econo-Pac Q Cartridge

Data submitted by Dr. Ivan Kaiser, University of Wyoming,Laramie, WY USA Dr. Ivan Kaisers research involves the isolation and purification of proteins from snake venom, such as post-synaptic neurotoxins and hemorrhagic factors. In addition to research, Dr. Kaiser teaches a course on protein...

Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

Theresa Redila-Flores, Bio-Rad Laboratories, 2000 Alfred Nobel Drive,Hercules, CA 94547 USA The Bradford and Lowry assays are 2 colorimetric assays that are commonly used to quantitate protein concentration. Bio-Rads protein assay (catalog #500-0001), based on the Bradford dye-binding assay (Bradford 1976), measures t...

Detection of Alprazolam and Temazepam in a Bovine Plasma Matrix Using the Varian 500-MS Ion Trap Mass Spectrometer

Alprazolam (Xanax) and Temazepam (Restoril) were analyzed in a Bovine Serum Albumin (BSA) matrix, both before and after protein precipitation using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC/ESI/MS/MS). The ability of the Varian 500-MS IT...

Fast Analysis of Phenoxyacetic and Phenoxypropionic Herbicides in Vegetable Matrix by Negative Ion Electrospray LC/MS/MS

C. Schauber and T. Faye, Varian, Inc., France Phenoxy acids are used as herbicides in agricultural and domestic areas. These compounds have to be detected at trace level to ensure a safe drinking water and food supply and verify appropriate remediation of these...

Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum

One of the major difficulties in analyzing the proteome of human serum is the dynamic range of the concentrations of the proteins present in the sample. Human serum albumin (HSA) constitutes 57-71% of total serum protein and gammaimmunoglobulin (IgG) ranges from 8-26%. Removal of these two proteins alone clears about 75% of t...

Cyclodextrin enhances room temperature phosphorescence

Cyclodextrin-enhanced room-temperature phosphorescence (CD-RTP) is an analytical technique that offers well-resolved spectra and subpicogram detection limits. When polynuclear aromatic hydrocarbons (PAHs) are included in cyclodextrin molecules in the presence of a heavy-atom moiety such as 1,2-dibromoethane, they exhibit intense, well-defined...

Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms

Tunable Parameters of the Affymetrix probe set data to generate expression values (Signals and LogRatios), Detection and Change calls, and associated p-v...

The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range

New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range platform as an integrated genetic analysis system, supporting continued...

The New Affymetrix GeneChip Scanner 3000

Instrument System is the most advanced microarray analysis platform available and reduces the time and cost of discovery research. Improvements in array manufacturing pr...