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Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems

Assess the In Vivo Activation of Signal Transduction Pathways withPathDetect Stratagene Signal transduction is essential to many cellular processes, such asgrowth, differentiation, an...

Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line

midiprep kit Stratagene EF plasmid midiprep kit, a kit based on anion exchange chromatography, magneticbeads, and cesium ch...

Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance

Stratagene We introduce six pairs of control RT-PCR primers that are designed todetect specific human gene transcripts with various expression levels. Theseprimer sets provide insight into whether cDNA synthesis has been effective forall ab...

Highest Possible Transformation Efficiencies for High-Throughput Applications

Convenient, time-saving 96Pack Gold competent cells streamline transformation procedures Julie Bonnardel Ronda Allen Bruce Jerpseth Sean Banks...

Optimizing Transfection Conditions for Studying Signal Transduction Pathways

Optimizing Transfection Conditions for Studying Signal TransductionPathways in vivo reporting systems Stratagene Stratagenes PathDetect <font face="S...

Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements

Stratagene Cloning Systems, Inc. Enhancer elements are the convergent points for many intracellular signaltransduction pathways. For simple, rapid and convenient assessment of the invivo activation of these pathways, Stratagene has d...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines for...

Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells

Department of Cell Biology, Neurobiology, and Anatomy University of Cincinnati College of Medicine, Cincinnati, OH 45267 Transfection of mammalian cell lines is enhanced by the use ofliposome-based reagents. Here we compare the transfection e...

Versatile Reporter Vectors for Monitoring Viral Transduction

Determine retroviral transduction efficiency and gene expression invirtually any target cell line Stratagene , pFB-Neo-LacZ <font...

Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts

reagent Genetic Applications LLC Stratagene transfection reagentpromotes efficient transfection of human dermal fibroblasts. Twenty minutes...

In Vivo Signal Transduction Pathway Reporting Systems

Stratagene Cloning Systems, Inc. Stratagene's PathDetect in vivo signal transduction pathway reportingsystems are designed for specific, rapid and convenient assessment of the invivo activation of signal transduction pathways. These r...

Improved Vectors for PathDetect Trans-Reporting Systems

Stratagene Stratagenes PathDetect in vivo signal transduction pathwayreporting systems can be used to assess the in vivo activation of signaltransduction pathways. To expand the utility of these systems, Stratagene isreplacing the pFA-cJun...

Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance

Stratagene transfection reagent offersimproved, consistent transfections for both adherent cells and cells growing insuspension. In this article, we summarize the results of several experimentsthat characterize the eff...

Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells

Stratagene Cloning Systems Clonetics Corporation, Walkersville, Maryland LipoTAXI transfection reagent is a novel liposome reagent that offerslow cellular toxicity and high transfection efficiencies for adhe...

New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

Stratagene cis-reportingsystems now includes the GAS and ISRE reporting systems, to better study theactivation of signal transduction pathways stimulated by int...

Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells

Mycoplasma PCR Primer Set for fast and easy detection of mycoplasmacontamination Yale University Genetic Applications LLC Stratagene Mycoplasma contamination of mammalian cell cultures can substantiallyreduce the potenc...

New Competent Cells for Highest Transformation Efficiencies

Stratagene Cloning Systems, Inc. Stratagenes new, high-efficiency competent E. coli cell linesignificantly improves the transformation efficiency of ligated DNA. EpicurianColi ultracompetent cells* yi...

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

Stratagene Cloning Systems, Inc. The PathDetect in vivo signal transduction pathway reporting systemsare designed for assessing the in vivo activation of signal transductionpathways. The PathDetect trans-reporting systems include a unique fusion trans-activ...

High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

Nonliposomal formulation yields high efficiencies in a variety oftransfection conditions Stratagene Introducing foreign DNA into eukaryotic cells requires high-potencytransfections, which demand superior quality transfection reagents that areefficient, have low toxicity, and wor...

Transgenic Animals

The term transgenic animals describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in the host animal. For this reason, mice, rats or other small mammals are used to introduce foreign DNA...

Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending)....

Efficient Transfection of Neurospora Crassa

Katherine A. Borkovich PhD, University of Texas-Houston Medical Center, Dept. of Microbiology and Molecular Genetics, Houston, Texas. Kimberly Vollmer, Brinkmann™ Instruments Inc., BioSystems Application Lab, Westbury, New York....

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending)....

Efficient Transfection of Neurospora Crassa

Katherine A. Borkovich PhD, University of Texas-Houston Medical Center, Dept. of Microbiology and Molecular Genetics, Houston, Texas. Kimberly Vollmer, Brinkmann™ Instruments Inc., BioSystems Application Lab, Westbury, New York....

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

TransMessenger Transfection Reagent

For transfection of cells with siRNA in RNAi procedures Tested for a...

Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures

Jochen Meier, Ivonne Strmel, Radu Iosub, Sonja Schmidt, and Rosemarie Grantyn Developmental Physiology, Johannes Mller Institute, Humboldt University Medical School (Charit), Berlin, Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance...

Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent

QIAGEN GmbH, Hilden, Germany Primary neurons are extremely difficult to transfect, making genetic modification of neuronal cells by delivery of either siRNA or plasmid DNA challenging ( ). Recently, Krichevsky and Kosik report...

GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip expression arrays. The GeneChip Sample Cleanup Module was developed as part of an on...

Effectene Transfection Reagent

For transfection of a wide variety of cells, particularly effective for primary cells <l...

PolyFect Transfection Reagent

Optimized for transfection of COS-7, NIH/3T3, HeLa, 293, and CHO cells...

SuperFect Transfection Reagent

The proven activated dendrimer for transfection of a broad range of cell lines Ser...

TransMessenger Transfection Reagent Handbook

<a href="#T...

Transfection Reagent Selector Kit Handbook

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TransMessenger Transfection Reagent

For transfection of cells with siRNA in RNAi procedures Tested for a...

Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*

Center for Neurologic Diseases, Brigham and Womens Hospital, Harvard Medical School, Boston, MA, USA Genetic manipulations using neuronal cells are notoriously difficult. Therefore, efficient delivery of short interfering RNA (siRNA)...

Suggested applications for reverse transcriptases

High fidelity cDNA for cloning Amplification of viral RNA </s...

Properties of Reverse Transcriptases

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Reverse transcriptases suitable for one-step RT-PCRb

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