Tissues

Genomic DNA Preparation from RNAlater Preserved Tissues

The following protocol is suitable for 100 mg tissue samples. Mouse kidney, lung, and spleen genomic DNA have been successfully extracted with approximate yields of 70 g DNA per 100 mg tissue....

Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation

Tissue Collection: RNA Stabilization Solution for Freshly Dissected Tissue and Cells. Successful RNA isolation requires rapid processing or appropriate storage of the tissue or cell samples. Tissue samples must be quickly dissected...

Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix

Paul Burr, Wellcome Clinical Scholar, Department of VeterinaryPathology, University of Glasgow, Bearsden Rd, Glasgow. G61 1QH. A method that can be conveniently used to prepare DNA from a wide range of tissues for analysis in the Polymerase Chain Reaction is described. The method is both quick...

Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System

Nicholas N. Lyssenko and Mark L. Tucker, Soybean and Alfalfa Research Lab,USDA, ARS, Bldg. 006, BARC-West, Beltsville, MD, USA 20705 In 1987 Jefferson et al. described the use of β-glucuronidase (GUS) as a marker to study gene expression in plants. Expression of GUS activity in plant tissu...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

This application note describes a method for extracting and isolating total RNA from rodent tissues that have been fixed in formalin and embedded in paraffin, and the subsequent amplification of mRNA from these tissues for use in microarray experiments. We have used this methodology to demonstrate that robust and reliable methods do exist for retrieval of...

Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data

Microarrays offer a powerful means of studying human gene activity and thereby providing insight into cellular, molecular, and biological changes. Applied Biosystems has developed the Expression Array System, a complete system for gene expression studies. It includes Human Genome Survey Microarrays (utilizing fully curated transcript data...

Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

James F. Eliason, Ph.D., Asterand plc The path to identification of new and improved drugs for the treatment of human diseases often begins with the identification of novel gene targets through RNA expression analysis. Most high throughput gene expression profiling studies use high density microarrays, wh...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

S. Ambrose (1936-2002) Ernest Mueller Preserved samples from medical, forensic, museum and other archival collections representa rich source of study material, much of it meticulously collected, characterized andpreserved through many decades of work by experts in the field. Wh...