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Map and Link Human Genetic Disorders with SSLP Analysis

system Medical Genetics Branch, National Human Genome Research Institute NationalInstitutes of Health, Bethesda, MD system is idealfor analyzing SSLPs (simple sequence-lengt...

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

Optical Filter Optimized for Imaging Silver-StainedGels Stratagene II Still Video System that increases contrast...

Genetic Reporter Systems

Genetic reporter systems have developed into an essential tool for examining regulatory promoter and enhancer sequences as well as transcription factors. In most cases, the element under investigation (promoter, enhancer) is cloned together with the reporter gene in an expression vector, which is subsequently used to tra...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

Genetic Reporter Systems

Genetic reporter systems have developed into an essential tool for examining regulatory promoter and enhancer sequences as well as transcription factors. In most cases, the element under investigation (promoter, enhancer) is cloned together with the reporter gene in an expression vector, which is subsequently used to tra...

Eppendorf Electroporation Application Notes Your participation is highly welcome !!!

E-mail us your application notes for inclusion in this Eppendorf web page. Each contribution published will be acknowledged with a set of electroporation cuvettes. ...

Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells

<A href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus pleuropnomoniae <A href="http://www.biocompare.com/techart.asp?id=576">Actinomyces viscosus <A...

An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat

GBF Gesellschaft fr Biotechnologische Forschung mbH (Society for Biotechnological Research) DNA microarrays are a key technology in modern molecular biotechnology. Due to their high degree of parallelism, they allow the...

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Sonja Vorwerk, Max Planck Institut fr Zchtungsforschung, Carl-von-Linn Weg 10, 50829 Cologne, Germany Phone: ++49-221-5062-441, Fax. ++49-221-5062-413, e-mai...

Critical factors for successful microarray and real-time PCR analyses

QIAGEN GmbH, Hilden, Germany Gene expression profiling at the RNA level has been significantly facilitated by microarray analysis and quantitative real-time RT-PCR. Microarray analysis offers the advantage of profiling expression levels of hundreds or thousands...

Analyzing Trace-Level Impurities of a Pharmaceutical Intermediate Using an LCQ Deca Ion Trap Mass Spectrometer and the Mass Frontier Software Package

Mark R. Kagan , Carrie Liu 1Thermo Finnigan, Piscataway, NJ; 2Thermo Finnigan, San Jose, CA; 3Eisai Research Institute, Andover, MA The data presented here can be a...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

Mark Churchill and Mark Harrison, Thermo Finnigan, Hemel Hempstead, UK The data presented here can be acquired using a Thermo Finnigan TSQ Quantum mass spectrometer...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

One of the RNA silencing products available from Ambion is a kit for the synthesis of siRNA, the <A href="http://www.ambion.com/catalog/CatNum.php?1620">Silencer <A href="http://www.ambion.com/catalog/CatNum.php?16000"> siRNA Construction Kit . While this kit is very cost effective...

RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater

is a tissue storage solution that stabilizes and protects cellular RNA in fresh tissues. (Ambion's new RNA -ICE is designed specifically for use with frozen tissues - see "<A href="http://www.ambion.com/techlib/tn/103/1.html">Stop Grinding...

RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

Gene arrays can be used to simultaneously measure the expression levels of thousands of genes in a single experiment. One limitation of this technology is that large amounts of RNA may be required for each array hybridization experiment -- typically 10 g of total RNA. This becomes problematic when working with limited sampl...

Acrylamide Polymerization A Practical Approach

Paul Menter, Bio-Rad Laboratories, 2000 Alfred Nobel Drive,Hercules, CA 94547 USA The unparalleled resolution and flexibility possible with polyacrylamide gel electrophoresis (PAGE) has led to its widespread use for the separation of proteins and nucleic acids. Gel porosity can be varied over a...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

Contributed by James V. Anderson and Charles L. Guy, The University of Florida, Institute of Food and Agricultural Sciences BiPs (Immunoglobulin Heavy Chain Binding Proteins, also known as GRP78; Glucose Regulated Proteins) are ER-luminal proteins and members of the HSP70 family. 1</...

Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE

David A. Crispin,* Ru Chen,* Michael B. Kimmey,** Teresa A. Brentnall** * Departments of Pathology and **Medicine, Division of Gastroenterology, University of Washington, Seattle, Washington. Introduction Pancreatic cancer is the fourth leading cause of cancer death in the U.S....

Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

Pipettors often fail to meet the manufacturers stated specifications and pipetting is a major source of laboratory error even when the pipettor is operating properly. Accuracy and precision specifications typically provided by pipettor manufacturers are obtained under ideal conditions. In the laboratory, performan...

A prognosis for GE and Abbott Diagnostics

It is a...

Kanavas calls for audit of data security practices

In the letter, addressed to <a h...

Silva-Craig joins Neurognostics board

Neurognostics, which spun out of the Medical College of Wisconsin in 2003, develops magnetic resonance imaging (fMRI) products and services Silva-Craig is the president and chief operating officer of Ema...

Medical College researchers receive new grants to enhance discovery of heart-protecting anesthetics

Zeljko Bosnjak, Ph.D., professor and vi...

Third Wave receives FDA clearance of pharmacogenetic test

It is the first pharmacogenetic test to be approved by the FDA for use as a companion diagnostic to a specific drug therapy. The test is c...

Midwest holds strong position in worldwide diagnostics business

To put diagnostics into perspective, Steve B...

Ready to leave? Why you shouldn't give two weeks' notice

The "Exit Stage Right" article on Why? Because giving two week's notice (let alone four week's notice) went out with nickel beers. The article also needs a reality check on some of its observations and assertions of how life is in today's corporate...

Third Wave continues molecular diagnostic focus with new CEO

MADISON , is transitioning to the position of executive chairman in order to focus on the assessment and development of next-generation technologies. In addition, the company announced that <a href="ht...

Diamond Film May Enable Critical New Sensors For Bioterror

MADISON - In this time of the chronic threat of terrorism and the possibility of war with an adversary who may be armed with biological weapons, high on the wish list of security agencies and battlefield commanders is a quick and easy way to detect the presence of dangerous biological agents. Now, with the help of a novel diamond film developed by chemists at the University of Wisconsin-...

Industry Veterans take a look back from 2025: Bio-Technology, and Bio-Informatics are the focus

SCOTTSDALE, Ariz. -- Panelists at the Demo 2003 conference offered insights into the next technology innovations by answering Producer, Chris Shipley's question, "If we look back in the year 2025 what will see as the big innovations?" Mitch Kapor, Chairman of the Open Source Applications Foundation, and founder of Lotus, began the discussion by stating, "I would be hanging out in the Bio...

Republicans criticize Doyles growth package

While Republican leaders in the State Assembly and Senate expressed support yesterday for Gov. Jim Doyles Grow Wisconsin program, Sen. Ted Kanavas -- a Brookfield Republican expressed support, but takes issue with the provisions for the state-funded venture capital authority. Kanavas reserved his stronges...

Roche Diagnostics Corporation

Advantages of Roche Applied Science amplification products <a href="http://w...

Roche Diagnostics Corporation

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Ampl...

Multichannel Pipetting: How to Choose the Correct Pipettor

by Sari Mannonen, Pasi Nieminen, Jukka Kaasinen and Kati Andersin Multichannel pipettors are widely used in microplate work, includingserological testing, molecular biology, immunology assays, andpolymerase chain reaction (PCR). The explosion of specific markets suchas genome projects and drug discovery has further increased the use ofthe microplate. Because mic...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

90% of mammalian RNA from complex mixtures of host-bacterial samples Seamless integration with MICROB <...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

Jaclyn Shingara, Kerri Keiger, Ila Wolf, Jeffrey Shelton, Emmanuel Labourier, and David Brown R&D Division, Ambion, Inc. MicroRNAs (miRNAs) are small, siRNA-like molecules, encoded in the genomes of plants and animals,...

Measuring RNase Activity A Real-time Kinetic Analysis

The RNaseAlert QC System, when used in conjunction with a fluorometer capable of kinetic analysis, provides a rapid and ultra-sensitive assay for determining RNase A specific activity. The RNaseAlert QC System also detects a variety of other nucleases, including RNase A, T1, RNase I, micrococcal nuclease, S1 nuclease,...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most common m...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most common m...