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Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression

Stratagene tissue arrayslides offer further refinement of tissue array technology through optimizedtissue fixation and processing procedures that preserve tissue morphology,antigenicity of pr...

Highest Possible Transformation Efficiencies for High-Throughput Applications

Convenient, time-saving 96Pack Gold competent cells streamline transformation procedures Julie Bonnardel Ronda Allen Bruce Jerpseth Sean Banks...

High-Throughput Cultivation of Bacterial Clones

Stratagene The tempest thermoshaker and a 96-deep-well round-bottom plate were used to cultivate bacterial clones in a...

High-Throughput Isolation of Total RNA

Stratagene 96 total RNA purification kitprovides a convenient method for simultaneous isolation of total RNA from up to96 cultured cell samples. Because RNA is bound to a solid support, there is noneed fo...

High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications

Simultaneous purification of 96 PCR products Stratagene Phenogenex 96 PCR purification kit is a newmember of the StrataPrep product line. This kit offers a high-throughput formatfor purifying ultra-pure PCR product DNA from la...

The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments (100 bp to roughly 2,000 bp). However, demanding...

High-throughput gene expression analysis to screen for anti-asthma drugs

Andreas Pahl, Uwe Oelmller,* and Susanne Ullmann Department of Pharmacology, University of Erlangen, Erlangen, Germany * QIAGEN GmbH, Hilden Germany Drug library screening based on gene expression analysis requires reliable and reproducible methods of RNA pu...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

QIAGEN introduces the DirectPrep 96 Miniprep System for cost-effective, fully automated, high-throughput DNA purification from high-copy plasmids. Use of a novel lysis chemistry eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using a single plate and a simple bindwashelute...

Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

Joseph W. Turek and Guy C. Le Breton, Department of Pharmacology,University of Illinois at Chicago, College of Medicine, Chicago, IL 60612.Address correspondence to: Joseph W. Turek, 835 South Wolcott, Departmentof Pharmacology M/C 868, University of Illinois at Chicago, Chicago, IL 60612.E-mail: jturek1@icarus.uic.edu Introduction...

The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research

Over the past decade, many organisms have been thesubject of large-scale genome projects, and as a result atremendous number of gene sequences are now readyfor functional analysis. Knowledge of tissues and cellsthat express particular genes is key to understandinggen...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

The efficiency of the Rapid DNA Ligation Kit fromRoche Molecular Biochemicals was compared withstandard and optimized conventional ligation procedures.The reactions generated circular recombinantbacteriophage DN...

High Throughput aRNA Amplification

Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis Includes ArrayScript, an engineered M-MLV RT, to maximize yields of full-length cDNA aRNA synthesis powered by MEGAscript High Y...

High Throughput siRNA Electroporation

Efficient siRNA delivery with minimal cell mortality Electroporate up to 96 samples at the same time Deliver siRNA into primary, hard-to-transfect, and suspension cells D...

High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

A variety of siRNA applications, including siRNA library screening, require the efficient delivery of hundreds to thousands of siRNAs to cells. Most published siRNA studies describe performing transfections in 6 well and 24 well formats. Ambion has developed two reagents, siPORT...

High Throughput RNA Isolation for High Performance Microarray Analysis

Protocol combines benefits of TRI Reagent sample lysis with MagMAX magnetic bead-based RNA isolation technology Procedure is compatible with fresh, flash-frozen, or RNAlater-treated tissues...

RNAi for High Throughput Analysis

Large scale gene expression studies require reliable high-throughput methods to synthesize and then purify high quality RNA from a large number of samples. Although the MEGAscript RNAi Kit includes reagents for both dsRNA production and clean up, it is not optimized for clean up in a 96 well plate forma...

Robust High Throughput RNA Isolation from Blood Samples

Mammalian whole blood is a widely used tissue for molecular assays due to abundant genetic information as well as ease and simplicity of sample collection. RNA isolation from whole blood is challenging, but Ambions MagMAX-96 Blood RNA Isolat...

Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

Tech/Aid, 7700 Edgewater Drive, Oakland, CA 94621 USA, and Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Running PCR samples on agarose gels is a routine yet timeconsuming procedure....

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

<P >J. Turner GE Healthcare, The Maynard Centre, Cardiff, UK Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ Kinase Assay Kits gene...

The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists

Beta-agonists are group A substances of the European guideline 93/257/EWG (revision 98/536/EG) having an anabolic effect and not licensed. Thus, they have to be determined in veterinarian samples like muscle, liver, retina, and urine. The coupling of High Pressure Liquid Chromatography (HPLC) and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS-MS) seems to be the analytical m...

Technical Specifications Accelerating Your Research with the BD FACSCanto High Throughput Sampler Option

Accelerating Your Research with the BD FACSCanto High Throughput Sampler Option The new BD High Throughput Sampler (HTS) provides fully automated and rapid sample acquisition. It is designed to speed through a microtiter plate in 15 minutes* with sample carryover to...

Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers

SSCP analysis is based on...

High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

Applied Science and Technology Department, Caliper Life Sciences, Inc., Hopkinton, MA 01748 Agencourt Bioscience Corporation, 100 Cummings Center, Suite 107J, Beverly, MA 01915 The goal for plasmid purifi...

Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery

Department of Drug Metabolism and Pharmacokinetics, Sch...

Three Micron TSK-GEL ODS-100V Columns for High Throughput LCMS

It is well known in HPLC that columns packed with smaller particles are more efficient at the expense of higher back pressure compared to columns packed with larger particles. The gain in efficiency is usually traded off by reducing column length, which in turn results in shorter analysis...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

S. Ambrose (1936-2002) Ernest Mueller Preserved samples from medical, forensic, museum and other archival collections representa rich source of study material, much of it meticulously collected, characterized andpreserved through many decades of work by experts in the field. Wh...

Three finalists named for UW-Madison CIO

The finalists are Ronald D. Kraemer, deputy CIO and associate director of the UW-Madison Division of Information Tec...

Fusion 2007: IT threats make risk management paramount

The CEO's head, too. If there was a consistent theme during the , it was that threats are bombarding business organizations from many directions, and risk management has surpassed security as the paramo...

Third Wave Technologies to raise $14.9M through private debt

According to Third Wave, the notes are convertible into approximately 2.48 millio...

Raising capital through placement agents

Editor's note: After more than a year-long "break" in Iraq, Matt Storms is ready to continue his series of columns about raising capital. His previous columns covered due diligence and corporate clean up, the mechanics of raising capital, and Small Business Innovation Research (SBIR) and Small Business Technology Transfer (STTR) grants. We thank Matt for his service, and we thank him for the f...

Power services firm JT Packard isn't through growing

As founder of , Cason presides over a rapidly expanding power service provider. The company has grown by 500 percent over the past three years, and now is the largest uni...

Mithridion wins grants for Alzheimer's research

The twin grants, awarded by the , a branch of t...

Cutting through the blog fog

suggesting...

Growth through the eyes of GE's Immelt

GE is a world-class company and object of emulation for many. It is also a unique organization. The company is a true conglomerate, but one that is more than an investment portfolio. It operates in a diverse set of businesses ranging from aircraft engines and massive power-generating turbines to financial services and broadcasting. It is huge - $150 billion in reven...

New book from former CDC CEO walks readers through innovation

MINNEAPOLIS - I recently interviewed Robert M. Price about his book "The Eye For Innovation: Recognizing Possibilities and Managing the Creative Enterprise". This column includes his comments about business, strategy, innovation and "the `C' word". "At its most basic level," he writes, "business strategy is about people." Not surprisingly, Price's people orientation is evident...

Why Milwaukee needs its own thriving research base

Don't suggest to UW-Milwaukee Chancellor Carlos Santiago that research projects on his campus will get ahead by pilfering from programs at UW-Madison. He'll probably laugh at you. "Let's get real," Santiago told listeners at a recent Madison forum on the future of higher education in Wisconsin. "(UW-Milwaukee research programs) are not competing with UW-Madison. These are institutions at...

Three state businesses receive technology grants

Madison, Wis. - Three Wisconsin businesses will receive funding for technology development projects from the Wisconsin Department of Commerce under the Technology Assistance Grant program. The TAG program provides grants of up to $15,000 to early-stage Wisconsin high-tech companies to offset the cost of professional services. The latest group of grant recipients are: <a href="http://www....

Leading cancer institute to install three TomoTherapy systems

The , based at the University of South Florida in Tampa, will install three of TomoThera...

Fiserv crosses Fortune 500 threshold

Fiserv, which reported $4.1 billion in annual revenue and earned $516 million in 2005, cracked the...

Waukesha considers wireless bids from three firms

Three firms want to build the infrastructure on city property such as light poles, then wholesale bandwidth to Internet service providers. After waiting several years for the winning company to recoup some costs, the city would start to receive royalties....