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Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance

Stratagene transfection reagent offersimproved, consistent transfections for both adherent cells and cells growing insuspension. In this article, we summarize the results of several experimentsthat characterize the eff...

New Competent Cells for Highest Transformation Efficiencies

Stratagene Cloning Systems, Inc. Stratagenes new, high-efficiency competent E. coli cell linesignificantly improves the transformation efficiency of ligated DNA. EpicurianColi ultracompetent cells* yi...

Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures

Jochen Meier, Ivonne Strmel, Radu Iosub, Sonja Schmidt, and Rosemarie Grantyn Developmental Physiology, Johannes Mller Institute, Humboldt University Medical School (Charit), Berlin, Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance...

Effectene Transfection Reagent

For transfection of a wide variety of cells, particularly effective for primary cells <l...

Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A

The macular region of the human retina is responsible for almost all color vision and most of the high resolution visual acuity. This part of the retina, known as the macula lutea, or yellow spot, accumulates approximately 200 ng of two closely-related compounds, lutein and zeaxanthin....

Stratagene to pay Third Wave $10.75M in patent case

The case, which was litigated in U.S. District Court for the We...

eBay decision changes balance of power in patent disputes

. The first two parts, written by attorney Gina Carter, pertained to preventing intellectual property infringement within organizations. The following commentary, written by attorneys Joe Goode and Jonathan Fritz, addr...

Drug innovation beats marketing, patents and mergers

While Genentech had revenues of $4.6 b...

Want a raise? Ten best midwest tech salary-increase pitches

You know you have to have a great argument for your boss to get him on board with your pay-raise proposal. You also know that dollars are tight and your boss is pre-disposed (or programmed after several years of heart-rending pleas fro...

Creating a public service for multimedia content

Founded amid the clamor and managing to duck the hype of peer-to-peer networking, Mike Homer's Kontiki has evolved into a rich media delivery platform based on a "grid network" to accelerate the delivery of video and audio content. A skilled market reader and a clever product director, Homer has guided Kontiki around the land mines of P2P, digital rights managemen...

Carl Christensen says Marshfield Clinic's history gives it an IT edge

In the last decade, technology applied to medical records and other information has become hot enough to raise a new term, "medical informatics," to more common usage. At the <a href="http:...

High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

By Susan Catalano, Ph.D., Acumen Pharmaceuticals, Sylvia de Bruin, Joe Bosworth, Ph.D., Neal Gliksman, Ph.D., Kimberly Best, Ph.D. and Paula Rickert, Ph.D., Molecular De...

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

Amplification of DNA through the polymerase chain reaction (PCR) is a highly sensitive method capable of copying millions of DNA molecules from just a single molecule. Because of the extreme sensitivity of this method, any carry-over product from previous reactions can result in false positiv...

Is Z' factor the best assessment for the quality of cellular assays delivering higher content?

Amersham Biosciences UK Limited, Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA England As high throughput imaging systems, analysis al...

Extended data analysis using DeCyder EDA

DeCyder EDA • extended data analysis • workspace • base set • differential expression analysis • principal components analysis • pattern analysis • discriminant analysis • Ettan DIGE DeCyder™ Extended Data Analysis (EDA) Software was used to perform advanced statistics on samples fr...

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

5, a pH sensitive dye, has shown utility in β2-adrenergic receptor agonist screening (1). CypHer5 has been used to obtain dose-response and rank-order potency data for both agonist and antagonist treatment of β2-adrenergic receptor expressing cells (2). For live-cell receptor internalization studies,...

Detection of N-in-1 Experiments: Extending the Range of N Using the API 3000 LC/MS/MS System

A significant...

Document retention policy can get your arms around eDiscovery

As information that a business receives, creates and utilizes expands over time, so does the risk of loss of that information or control over it. Besides the practical rea...

Techware to extend broadband wireless network to Butler

The company, which started the build out in Germantown, said it will begin to deliver broadband service that is capable of providing speeds up to 14 <a href="http://en.wikipedia.org/wiki/Mega...

The Right Brain: A neurological solution to the flattening world

article titled It's a Flat World After All and its impact on the world of biotechnology. After returning from my visit to India this year, Friedman's thoughts brought new meaning to my persp...

Changes to U.S. patent law must nurture our culture of innovation

Even during an era when America-bashing is back in vogue, there are certain things this country does well - and has always done well - much to the consternation of our rivals and com...

WARF questions relevancy of documents used to uphold patent challenge

refu...

Kauffman study shows consistent entrepreneurial growth

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Stem cells get most attention, but they are only part of Wisconsin's bio-portfolio

Wisconsin's high-tech loss leader these days are human embryonic stem c...

Supreme Court limits foreign reach of U.S. patents

the Supreme Court delivered another blow by limiting their reach abroad and...

Cardinal Health likes growth potential of Viasys NeuroCare Group

As part of a merger agreement between Cardinal Health and Viasys Healthcare, Car...

High court sends message on patent obviousness

is the requirement that the claimed invention is not obvious under 35 U.S.C. 103. Similarly, obviousness is a typical defense raised in patent infringement cases. Yet, overcoming obviousness objections, both in the PTO and in...

Supreme Court ruling seen as blow to WARF stem cell patents

The answer is yes, according to the California consumer watchdog organization that is challenging the patents, but that's not necessarily a consensus opinion. The nation's high cou...

Patent licensing: For enforcing companies, the rules have changed

Typically, the patent owner would do this by sending out a carefully worded letter asking that a party agree...

Patent ruling doesn't tarnish Wisconsin's stem cell leadership

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Here's why Wisconsin's stem cell patents are being challenged

Not only does that make for appealing television drama, but it's pretty...

On the Web, attention spans shrink, the need for value rises

With these tools, everyone can have their 15 minutes - but just 15 minutes. Maybe not even that. It seems that 15 minutes is about all anyone has time for these days, at least in...

WARF is likely to hold on to stem cell patent rights

A look at the facts in the dispute over three important stem cell patents - and the history behind similar disputes - sho...

Patent office upholds challenge to WARF stem cell patents

Every WARF claim regarding the met...

Rocket docket speeds patent infringement suits

Intellectual property and science, particularly biotechnology, go hand-in-hand. In the highly competitive science and technology community, patents, copyrights, and trade...

Third Wave files countersuit vs. Digene in patent case

The countersuit was filed with the in Madison, whe...

Bio-energy and cleantech grab attention of investors

- In 2000, so-called cleantech investments made up only one percent of the venture capital market in the United States. By late 2006, that slice of the venture capital pie had grown to 14 percent. The message is clear: The markets are warming to technologies that produce alternative fuels, keep the air and water clean, and help conserve energy.<...

Third Wave responds to Digene patent lawsuit

(HPV) and would continue to develop products to detect high-risk infections.<...

Wisconsin court affirms damages in Innogenetics, Abbott patent case

Madison, Wis. - The The Madison-based court affirmed the damage award to , a biopharmaceutical company based in Gent, Belg...

Wisconsin judge dismisses patent suit vs. Google

U.S. District Judge John Shabaz has ruled that Google's and AutoLink products do not infr...