Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

Geoffrey Grove, Ed Alderman Applied Science and Technology, Caliper Life Sciences, Inc., Hopkinton, MA 01748 Monica Adams, Jason Brennan Development Integration, Amersham BioSciences, 800 Centennial Avenue, Piscataway, NJ 08855 Overview The TempliPhi DN...

Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix

Paul Burr, Wellcome Clinical Scholar, Department of Veterinary Pathology, University of Glasgow, Bearsden Rd, Glasgow. G61 1QH. Introduction A method that can be conveniently used to prepare DNA from a wide range of tissues for analysi...

Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

The endopeptidase originally isolated from the filtrate of the lower fungus Tritirachium album limber is recombinantly expressed in Pichia pastoris. Proteinase K is extremely effective on native proteins from different so...

High Pure PCR Template Preparation Kit

For isolating genomic nucleic acids for PCR, restriction enzyme analysis, and Southern blotting Catalog Number Pack Size ...

Expand Long Template PCR System

for amplification of 520 kb targets from eukaryotic genomes Cat. No. 1 681 834 100 units Cat. No. 1 681 842 2 x 250 units Cat. No. 1 759 060 10 x 250 units Description Unique enzym...

Biomimetic-engineering design can replace spaghetti tangle of nanotubes in novel material

...ices using a thermal material that naturally dissipates heat from the device's center through a hierarchical branched network of carbon nanotubes. The template for this thermal material's design is a living cell, specifically, the hierarchical protein networks that allow a cell's nucleus to communicate with t...

MIT uses nano-origami to build tiny electronic devices

...rnal magnetic field, creating a Lorentz force that lifts the face. This technique is a form of directed self-assembly, where the designer provides the template and then lets the device assemble itself. The folded shapes can be fabricated with a few different types of material, including silicon, silico...

Scientists publish complete genetic blueprint of key biofuels crop

...size difference between the rice and sorghum genomes, while also suggesting a common overall genome structure for grasses. "Sorghum will serve as a template genome to which the code of the other important biofuel feedstock grass genomes--switchgrass, Miscanthus, and sugarcane--will be compared," said Andre...

Semiconducting nanotubes produced in quantity at Duke

...tubes to form in long, parallel paths that will not cross each other to impede potential electronic performance. Their method grows the nanotubes on a template made of a continuous and unbroken kind of single quartz crystal used in electronic applications. Copper is also used as a growth promoter. Carbon n...

Fujitsu PalmSecure and HT Systems' PatientSecure Selected by BayCare Health System to Protect Patient Confidentiality and Prevent Medical Identity Theft

...more quickly and efficiently." Fujitsu PalmSecure uses a near infrared light to capture a patient's palm vein pattern, generating a unique biometric template that is matched against a database of enrolled users' palm vein patterns. This advanced, vascular pattern recognition technology provides a highly rel...

Principle of Recursive Genome Function Supersedes Dogmas; By Andras Pellionisz, Online Ahead of Print; (Scientific Visionary Vindicated)

...is illustrated in the fractal growth of cerebellar Purkinje neuron . Starting from a primary information packet, a Y-shaped, fractal protein template is constructed by a "forward growth" process - in accord with the traditional picture - via transcription of DNA to RNA (where, in turn, RNA...

Guided Surgery Technology Gaining Popularity in the Dental Implant Market

...a dental implant. Furthermore, many clinicians also use a surgical drilling template that is manufactured based on the digital x-ray of the patient's mouth. The drilling template is used during dental implant placement and allows dentists to know exactly...

Lilly CEO Urges Policy Prescriptions to Help Ensure Public and Private Biotech Investments Generate Dividends and U.S. Job Growth

...kers, business leaders and academics, under the theme "Economic Prosperity through Scientific Discovery." He suggested that the Boston area provides a template for other communities across America, citing reports from Northeastern University that show that Massachusetts has enjoyed a 10 percent rise in the nu...

Copolymers block out new approaches to microelectronics at NIST

...cheol Kim (working with a team that included researchers from the University of Maryland and IBM) has found that changing the surface chemistry of the template by making some parts hydrophillic and some parts hydrophobic also can elegantly control the dimension of the block-copolymer pattern relative to the c...

Microchip Biotechnologies, Inc. Collaborating With Stanford University on Developing a Front End Microfluidic-Based Sample Preparation System for Pyrosequencing Platforms

...evelop a front-end microfluidic based sample preparation system to generate template libraries for next generation Pyrosequencing. The goal of the project is to automate the complex, multi-step upstream template production using MBI's Microscale-On-chip-Valves (MOV(TM)) for Superscalar ...

deCODE Discovers Gene Variants that May Help to Distribute the Work of Evolution between Men and Women

...ng that there are hotspots and coldspots for recombination in all chromosomes, and that these are very different in women and men. This map provided a template for completing the final assembly of the sequence of the human genome. deCODE scientists then showed that recombination rate varies between families a...

Research by Case Western Reserve University, VA earns cover of prestigious science publication

...g nanoparticles are first assembled into a three-dimensional network through gelation of nanoparticle dispersion, essentially forming a template. This template can then be filled with any polymer of choice by exchanging the solvent with a polymer-containing solution. Through the use of this new technique, we...

Model for the assembly of advanced, single-molecule-based electronic components developed at Pitt

...e Journal of the American Chemical Society (JACS). The project presents a template for assembling molecules over troughs that are only as wide as a single ato...-shaped carbon molecules known as fullerenes, but the method can serve as a template for creating the molecule-scale wires from a broad range of organic molecul...

BioReliance Corporation Launches iNet(TM) for Online Results Tracking of Biological Safety Tests

...Customers are using the projected lab initiation and report completion dates to facilitate their project planning efforts. In addition, the electronic template used for sample submission has simplified their paperwork, while making the entire process more timely and manageable." Judith Apshago, Senior Direct...

NIST team develops novel method for nanostructured polymer thin films

...istances as small as five nanometers or equally regular arrays of nanoscale dots. Chemically removing the other polymer leaves the pattern behind as a template for building structures on the microchip. In traditional oven annealing the quality of the films is still insufficient even after days of annealing...

Aware Celebrates Fifteen Years of Innovation and Leadership in the Biometrics Industry

...compliance assurance, MINEX- certified fingerprint template generation and matching for biometric identity ver...tract and match" software, is approved in both the template Generator and Template Matcher categories. Aware X...8- compliant, MINEX-certified fingerprint minutiae template generation and matching for 1:1 biometric verifica...

Wisconsin Tourism Aided by Internet Technology

...egory. Businesses that wish to be listed on WisconsinGuide.com can build a spotlight ad or website using SiteBuilder Xpress software. They choose a template design, color and font, add their own photos or logos and text and answer a series of questions. Within 15 minutes they have a functional website. The...

DEMOLetter: Draining the Economic Swamp

...b sites. The creation tool is smartly designed and simple to use. You can post collections of photos in a variety of styles, including a photo journal template that lets you turn photos into stories. Rather than siphoning off revenue from reprint services, Homestead charges a flat monthly fee, ranging from...

Milwaukee-based eFilm software selected by InSight Health, announces Workstation upgrade

... to view and manipulate digital diagnostic studies. Software improvements include advanced volume rendering, an enhanced MPR tool, enhanced orthopedic template capabilities, customizable toolbars performance in on-demand image streaming access to FUSION PACS and integrated CD creation. Users can also examine ...

UW-Madison workgroup helps state companies tap the power of RFID

...ss the level of relevance of RFID to their business, a tool that helps companies analyze their operations to identify where RFID would fit best, and a template that helps companies develop a business case for adopting RFID. Rockwell Automation in Milwaukee has good reason to embrace the technology: Many of...

Physician mindset slows adoption of electronic health records

... presenting new tools with the daily routine of doctors in mind. One example comes up when doctors who run a general practice may not find a record template useful, because so many symptoms and patients present too much information to stuff into predetermined, labeled cells. In contrast, specialists workin...

ZoomAlbum operates virtually

...ed to create a three-inch square photo album that easily slips into a pocket or purse. The ZoomAlbum software helps you drag and drop photos onto a template that arranges them for printing onto the special glossy photo paper provided in the ZoomAlbum kit. Each sheet and thus each album holds 12 images. ...

IT workforce development: Losing the numbers game

...and health information technicians, 1,230 new jobs, $28,976. To spread the word about emerging growth opportunities, and combat the lingering media template that off shoring spells doom for IT careers, ITAWi is preparing a public awareness campaign to dispel the myths. Rice said the campaign will be target...

The business case for software applications

...ce, those deliverables will be evaluated in years three, four, and five. Cagigal said the company has established a pretty well buttoned up process template that minimizes errors and risk, and always delivers value - perhaps not always in the anticipated timeframe. The payback in some cases might take ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

.... The GenomePlex hybrid method has several advantages over the previously described WGA methods. It does not require template with long, amplifiable regions, as does MSD. The method does not rely on highly efficient digestion and ligation as does LAP amplification. Finall...

QIAGEN Fast Cycling PCR Kit

...g a novel, patentpending PCR buffer formulation that significantly reduces the time required to form the polymerase, primer, and template complex. This allows the polymerase to extend the primers during the PCR annealing step thereby dramatically reducing the durati...

REPLI-g Mitochondrial DNA Kit

...and strand displacement activity, REPLI-g DNA Polymerase is capable of amplifying up to 100 kb without dissociating from the DNA template and minimizes unequal sequence and locus representation compared to PCR-based amplification methods. Simple one-tube...

REPLI-g UltraFast Mini Kit

... and no amplification in the absence of template Versatility optimized protocols for ...uration of genomic DNA and avoids fragmentation of template DNA caused by heat denaturation methods...denaturation, avoiding fragmentation and damage of template DNA. Next the sample is neutralized, an...

QIAGEN LongRange PCR and 2Step RT-PCR Kits

... The unique zwitterionic formulation of LongRange PCR Buffer improves pH buffering at high temperatures (7294C), minimizing pH-driven template degradation. In combination with Q-Solution (provided), it further facilitates amplification of GC-rich and other difficult temp...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

... DNA sequencing is performed using conditions determined to be optimal for the Eppendorf Perfectprep BAC 96 kit. A volume of 10 μL of purified template DNA were used in all 384 sequencing reactions. The following sequencing reaction setup (Table 1) and cycling conditions (Table 2) were used: ...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

...vidual values in the Group Table, this can be easily accomplished as described below. When you create a group and assign sample locations in template Editor, SOFTmax PRO automatically creates a group table to list the sample names and the corresponding data. The actual values which appear in the gro...

Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

...ctions Step 1 In Plate#1s template Editor, select to create a new group (doing so wil...lumn #2 in the Instrument Setup dialog box. In the template Editor, assign the wells in the second column to t...calculating pathlength). Step 3 Open the template Editor, create a new group (doing so will cause a ...

Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)

...ally less accurate. Step 3 Use the template Editor to create a template showing where standards, blanks and unknowns wil...wn in Figure 1 and Figure 2 (page 3). In the template Editor, two Groups were created, named Prednisone ...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

...ngth gives approximately 25% higher sensitivity. Step 3 Create a template showing where standards and unknowns will be located in the Cuvette Set or ...tributes negligible absorbance at 820 nm. Step 3 Create a template showing where standards and unknowns will be located in the Cuvette Set o...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

... Step 3 Use the template Editor to create a template showing where standards, blanks, and unknowns wi...nks (plate, sample or group) are designated in the template and are subtracted after pathlength correction. In...