Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
Stratagene We introduce six pairs of control RT-PCR primers that are designed todetect specific human gene transcripts with various expression levels. Theseprimer sets provide insight into whether cDNA synthesis has been effective forall ab...Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
Stratagene mammalian expressionsystem allows tightc...Rapid PCR* Using Active Tube Temperature Control On Thermo's PCR Machines
Solutions Note The time taken to complete a standard PCRprotocol is becoming increasingly important forresearchers today. Whilst many manufacturers ofthermal cyclers focus on pushing the limits of ramprates, in this article we highlight how Thermo's approach of focussing on ach...Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
RNA interference is a powerful technique for geneknockdown experiments in academic research and animportant tool for target identification and target validationin therapeutic research. A growing number of different cell lines are us...Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
Antibodies are commonly used in diagnostics, as research tools(for example protein arrays) or as biopharmaceutical therapeutics. Inall cases the successful production of antibodies requires precise testingand quality control. The key quality criteria include: protein ident...Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
Whereas in the past mass spectrometers (MS) were confined to therealm of small molecules, the development of soft ionization methodssuch as matrix-assisted laser desorption/ionization (MALDI) and electrosprayionization (ESI) revolutionized the analysis of large biomolecule...Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
This Application Note describes how Gene Logic, Inc., has improved itsability to analyze the quality of total RNA and fragmented cRNA by usingthe Agilent 2100 bioanalyzer. The Eukaryote Total RNA Nano and Picoassays are currently being utilized to examine the 28S/18S ratio...Designing Controls for siRNA Experiments
Because of its apparent specificity, reproducibility, and ease of use, RNAi technology is greatly accelerating the functional characterization of disease-relevant genes for drug discovery, target validation, and basic research efforts. To ensure the validity of RNAi...RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
RNase activity varies dramatically across different tissues. A comparison of total RNase activities for 8 different mouse tissues showed that total RNase activity spans a 181,000-fold range from pancreas to brain (Figure 1), which highlights the level of RNase control required for succ...Long-Term Instrument Control and Connectivity Solutions
The key to developing long-term instrument control applications is flexible and scalable software and hardware that enable seamless connectivity to any instrument in your application and offer built-in capabilities for advanced data analysis and presentation. Your choice of instrument should not limit or control your software choice, and your software preference...Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples
James F. Eliason, Ph.D., Asterand plc The path to identification of new and improved drugs for the treatment of human diseases often begins with the identification of novel gene targets through RNA expression analysis. Most high throughput gene expression profiling studies use high density microarrays, wh...Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
Researchers in New Brunswick Scientific's in-house labs have developed a unique controlstrategy to achieve high antibiotic expression levels in a pilot-scale fermentation process ofStreptomyces hygroscopicus. Using the BioFlo 6000 fermentor's on-line controller withtouchscreen interface augmented by the powerful supervisory capabilities of AFSBioCo...Chemical-petroleum executive joins Virent Energy board
Virent, which recently entered into an agreement with , a subsidiary of Shell Oil Co., is developing technologies that allow the renewable...Rockwell Automation to acquire British process-control firm
Headquartered in Maldon, England, ICS trades and does business as , a global supplier of critical control and safety soluti...DHC 2007: Controlling health IT costs the key to capturing value
Why that distortion exists could be the subject of an entire conference, and it occupied a fair amount of time during Digital Healthc...Johnson Controls deals for asset-tracking software
The Milwaukee-based Johnson Controls, which provides systems, technology, and services to buildings - inclu...Johnson Controls adds renewable energy unit
The company's new renewable technologies unit will focus on geothermal, solar, biomass, wind, and other alternative sources of e...Johnson Controls unit lands contract for plug-in hybrid battery
Under the terms of the agreement, <a href="http://www.chall...Corporate control begins with a strong board
Question: What is the most important corporate co...Net neutrality: To control or not control, that is the question
, D-Massachusetts, will bring this hotly contested issue to th...Johnson Controls to expand in Europe
In an arrangement the company is calling "a preliminary understanding,"...Johnson Controls partners on car satellite linkup
Johnson Cont...Florida schools employ Johnson Controls technology to stop sex predators
Johnson Controls, known more for its attempts to develop hybrid car batteries and energy-efficient building systems, said the security sys...Johnson Controls partnership wins new contract
The <a href="http://www.uscar.org/consortia&teams/consortiahomepages/...Johnson Controls appoints Roell to head EOC
Steve Roell, vice-chairman and executive vice president, was named chairman of the EOC, a committee comprised of senior leaders. An emplo...Johnson Controls reports record quarterly sales
The annual profit outlook, however, is getting dimmer due to the continuing slump in North American automotive int...Research park adds Johnson Controls, Eaton
will have sales offices in the park, according to Dave Domres, vice president of asset management for...Voice-changing software could make you a troll online
"Gamers can now change their voice to sound like a troll, giant, dwarf or dark lord," said Mark Ramirez, CEO of Screaming Bee, in a p...Control the community or just let go?
That advice may never have been so apt as in the case of Wetpaint, which t...Liquid crystals show promise in controlling embryonic stem cells
Liquid crystals, the same phase-shifting materials used to display information on cell phones, monitors and other electronic equipment, can also be used to report in real time on the differentiation of embryonic stem cells. Differentiation is the process by which embryonic stem cells gradually turn into function-specific types of adult cells or so-called "cell lineages," including skin,...Bush praises Johnson Controls battery tech in Milwaukee visit
In an address before employees at Johnson Controls, a Milwaukee-based automotive battery manufacturer that is developing...Johnson Controls makes wireless partnership
The SpotCell in-building wireless systems broaden coverage and improve signal strength in specific areas of a facility. This allows people to use their cell phones, PDAs, 3G-enabled devices, and mobile enterprise applications indoors without thick walls blo...Johnson Controls buys stake in health-care tech integration firm
, in Boca Raton, makes a connectivity suite for health-care organizations. Its products will h...Johnson Controls sales up, profits down
That was despite earnings growth bolstered by quarterly sales of $7.5 billion, up from $6.6 billion a year ago, partly from acquisitions of York International and Delphi's battery business....Wisconsin State Patrol goes high-tech with e-tickets
People have come to take for granted the increasing computerization of society. For example, e-tickets for airline flights are now commonplace. But how about e-tickets for speeding or running a stop sign? Since 1996, Wisconsin State Patrol vehicles have been progressively fitted with more advanced computer systems, utilizing laptop computers connected over a radio-based IP solution. Inst...Johnson Controls jumping further into hybrid-vehicle battery world
The new lab comes amid the rising interest in hybrid vehicles that are powered by batteries and gasoline. With Japanes...Johnson Controls introducing new building controls system
The Facility Explorer system is a hardware and software solution that is scalable to provide control to a wide variety of buildings, wheth...