TOS

hNT BlueNeurons Express b-Galactosidase at High Levels

-Galactosidase at HighLevels Layton BioScience, Inc. Gilroy, California Stratagene's hNT BlueNeurons express beta-galactosidase at h...

RT-PCR Primers for the Study of Apoptosis

Wei-ping Yang Danny Hoang Douglas McKenzie PeterPingerelli Stratagene The apoptotic response is a complex biological process that requires theregulation of a variety of genes that interact with and respond to intracellularand extracellular stimuli. Stratagene is introducing RT-PCR primer<sup...

A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage

Detection of early apoptotic events has become quiterelevant since many studies have demonstrated thatabnormalities in cell death regulation cause a variety ofdiseases and pathological conditions accompaniedeither by cell accumulation (e.g., cancer) or by cell loss(e...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer canbe used to analyze apoptotic cell samples. Performance of two assaysfor apoptosis detection is demonstrated and the data are compared tothose obtained with a conventional flow cytometer. Histogram quality...

Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer

A simplified and fast protocol for the analysis of DNAfragmentation during apoptosis This Application Note describes a simplified and fast protocol for apoptosisDNA fragmentation analysis with the Agilent 2100 bioanalyzerusing the DNA...

Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

Volker Wippersteg, Katja Kapp, Werner Kunz, and Christoph G Grevelding,Institute for Genetics, Center of Biological and Medical Research,Heinrich-Heine-University, D-40225 Dsseldorf, Germany Schistosomes are parasitic helminths that cause bilharzia (schistosomiasis) in humans, a tropical disease of worl...

The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays

Apoptosis represents a gene-directed, morphologically and biochemically distinct form of programmed cell death. Inappropriate control of apoptosis is associated with a variety of disease states such as cancer, AIDS, neurode...

The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System

Somatostatin (SS) is a neurotransmitter/hormone with a wide range of biological actions (1–3) and a reduction in cortical SS levels has been reported in Alzheimer’s and Parkinson’s diseases. The SS analog...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse Department of Biochemistry and Molecular Biology, Monash University,...

Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays

The understanding of the cellular signaling processes leading to programmed cell death (apoptosis) is of utmost importance in the study of autoimmune diseases such as rheumatoid arthritis or Parkinsons and Alzheimers disease. Here, errors in the signaling cascade are believed to lead to premature apoptosis in the affected t...

Counting Cytostar-T scintillating microplates on the MicroBeta JET

JET is a multi-detector...

Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard

Thermo Electron Corporation, San Jose, CA, USA The Deferred Standard (DS) concept was introduced more than 40 years ago with the purpose of monitoring the reliability of process chromatography. The DS consi...

Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar

Promega Corporation, Madison, WI, USA Application Note 138 Rev. 07/2006 Correlated measurement of an apoptosis marker (or caspase activity) and cell viability Monitoring of both lumi...