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A Positive Selection Assay for Mutation Analysis in Big Blue Animals

Select-cII mutation assay kit Animals Stratagene Stratagene now offers an alternative way to perform mutational analysis inthe Big Blue<sup...

Organize Your Data from the Big Blue Mutation Assay

Stratagene Solana Consulting Group, Solana Beach, CA Stratagene introduces the MutaTrak electronic notebook to record BigBlue transgenic rode...

Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations

Xiuyuan Hu Beti Belachew Lingyu Chen HaoqiangHuang Jason Zhang Stratagene can distinguish human DNA specimens that differ by a few nucleotides or only asingle nucl...

Using XL1-Red Mutator Strain to Generate Esterase Variants

XL1-Red cells allow isolation of gene productswith altered activity Markus M.Enzelberger Institute for Technical Biochemistry, University of Stuttgart, Stuttgart,Germany Institute for Industrial Genetics, University of...

Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer

transgenicrodent mutation assay Centre for Environmental Health, University of Victoria, Victoria, BC Canada transgenic rodent mutationassay,* which carries the E. coli lacI transgene in a retrievabl...

Mx4000 Multiplex Quantitative PCR System*,PPP

Stratagene has improved upon currently available QPCR machines with the Mx4000 multiplex quantitative PCR system. This new system offers better performance and functionality at a more economical price. Real-time quantitative PCR (QPCR) allows researchers to quickly and easilyquantify nuclei...

The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis

Karen Sermon, M.D., PhD Center for Medical Genetics, University Hospital and Medical School of the Dutch-speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium Preimplantation diagnosi...

BioRobot MDx Workstation

Fully automated sample preparation for clinical laboratories. Standardized processing and proven QIAamp chemistries for reliable results QIAsoft MDx software for protected protocols and secure user environments...

Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer

Nicola Hughes, Jean-Jacques Dunyach, 1Biovail Contract Research,Toronto,Ontario,Canada; 2Thermo Finnigan,San Jose,CA,USA; 3Therm...

Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer

Mark Churchill , John Henninge, 1 Thermo Finnigan, Stafford House, 1 Boundary Park, Boundary Way, Hemel Hempstead, HP2 7GE, UK; 2 Aker University Hospital, Hormone Laboratory, Section for Doping Analysis,Trondheimsv...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

Mark Churchill and Mark Harrison, Thermo Finnigan, Hemel Hempstead, UK The data presented here can be acquired using a Thermo Finnigan TSQ Quantum mass spectrometer...

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are! Choose the LightCycler PCR System, the only instrument flexible enough to meet your changing research needs. Central to the LightCycler System is the LightCycler Instrument which is much more than "Just an...

Comparison of Different Methods of mRNA Quantitation

<TABLE width="96%" border=0 align="left" cellPadding=8 cellSpacing=1> Nuclease Protection Assays <b...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

Michael Hepburn and Glenn A. Miller, Ph.D., Molecular Profiling Laboratory, Genzyme, Framingham, MA The search for sequence variations in genomic DNA is important in the study of genes that play a role in the development of cancer and a variety of other single and multiple gene disorders...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

Martin A.O.H. Menke*, Axel Reinecke-Lthge*, Barbara Mllmann*, Angela Kellner, Jutta Lttges*, Gnter Klppel* * Department of Pathology and Department of Hematopathology, Christian- Albrechts University Kiel, Michaelisstrae 11, D-24105 Kiel, Germany. Introduction Genetic alterations/variations are...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

Terry H. Landowski, Ibrahim Buyuksal, and William S. DaltonH. Lee Moffitt Cancer Center and Research Institute, University of SouthFlorida, 12902 Magnolia Drive, Tampa FL 33612 Introduction Identifying of mutations within specific genes has become an important strategy in determining the diagnosis and prognosis f...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

, and Jef Raus , 1 Dr. L. Willems-Instituut and 2 Limburgs Universitair Centrum, Universitaire Campus, B-3590 Diepenbeek, Belgium Introduction Phenylketonuria (PKU) is a hereditary disease that gives rise to elevated blood leve...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the OliGreen Pro software from...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell number.ATP measurements are used to monitor raw materials and manufacturing...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simplest and has recently gained widespread popularity in the microplateforma...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimize experimental variability caused by differences in cell number, viabili...

Gq-coupled Receptor Assays Using FLEXstation

Departments of...

Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

SIMPLE, RAPID EXPERIMENTAL SET-UP AND DATA ANALYSIS DETERMINE EC EXCELLENT FOR ASSAY DEVELOP-MENT AS DEMONSTRATED BY Z FACTOR INTROD...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

Simon Lydford & Petra Averbeck, Molecular Devices Limited, U.K. The FlexStation scanning fluorometer integrates sensitive optics, fluid transfer and temperature control, making it ideal for kinetic, cell-based fluorometric assays, such as the measurement of intracellular calcium, membrane potential and intracellular pH. <...

Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR

SIMPLE, FAST EXPERIMENTAL SET-UP Activation of G -coupled...

96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation

Initially, work in 96 well microplat...

New Sonic Foundry search engine finds words, phrases in video presentations

The Madison-based business is marketing the e...

Oskar Anderson to succeed Miszewski as head of state technology division

Anderson, who is on vacation this week, will join the <a href="http://ww...

Real pulse of the economy? Check real estate foreclosures

annual forecast for this year, I thought it would be a good idea to go back and look at the foreclosures on houses. You would think that by now the traction of the economy would grip and people would start to be more financially secure, but it does not look tha...

State's investment in early-stage businesses is changing perceptions

Attorney Joe Hildebrandt has been a part of many deals connecting promising young Wisconsin companies with investors, some close at home and others in the financial centers of the nations East and West coasts. Over the years, Hildebrandt told a legislative committee last week, he's battled the notion that Wisconsin is a "flyover state" that nei...

Start-ups, angels can connect on new state Web site

Wisconsin entrepreneurs can submit executive summaries of their businesses for angel investors' perusal to a new state Web site beginning May 9. The The WAN website, which is being ca...

CTT - Bridging the states entrepreneurial gap

MADISON In the past, when entrepreneur Ken Schlager started a company, he looked to the west or east coasts for funding sources. This time around, Schlager is turning to the Center for Technology Transfer (CTT) in Madison for help as he launches a cutting-edge technology that applies electromagnetic fields to wastewater treatment. Schlager, a 75-year-old electrical engineer, has develop...

Distance Education Conference Selects Sonic Foundry to Make Keynotes, Panel Presentations Available On-Demand

MADISON, WI Aug. 11 Sonic Foundry a rich media solutions company, today announced the 19th Annual Conference on Distance Teaching and Learning will use its Mediasite Live rich media Web presentation system to capture the keynote addresses and panel presentations, and make them available on-demand via the Web and on CDs containing this year's conference proceedings. Held Aug. 13-15 at th...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...

Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples

MIA (Melanoma Inhibitory Activity) was cloned as asecreted protein from human melanoma cell lines [1]. MIAis expressed in and secreted from malignant melanomasand chondrocytes [2, 3]. As MIA expression in chondrocytesis dependent on the differentiation status of the cel...

Comparison of different protein quantitation methods

This application note compares the accuracy and reproducibility ofabsolute protein quantitation of the Agilent 2100 bioanalyzer in combinationwith the Protein 200 Plus assay to the batch-based methodsLowry and Bradford as well as to denaturing gel-elctrophoresis (SDSPAGE)....

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

This application note describes how the Agilent 2100 bioanalyzer canbe used in combination with Standardized RT (StaRT)-PCR reagentsfrom Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville,AL, USA) to obtain highly reproducible, standardized gene expressionme...

Mutation detection for the K- rasand P16 genes

Mutations in the K-ras gene codon 12 region can lead to cancer, forexample of the colon, pancreas, liver, spleen, stomach or lungs. TheCDKN2A/P16 gene is a familial melanoma gene. Routine PCR and DNAsequencing methods can identify exactly which point mutation is present...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

Bio-Rad Laboratories, Paul Zoller, Theresa Redila-Flores, Dan Chu, andAsmita Patel, Ph.D. As the number of diseases and disorders linked to gene mutations grow, the need for scanning single-base mutations becomes ever more vital to extending understanding of disease-risk predictor mutations and their...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

MJ Research, Inc., South San Francisco, CA We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green...