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Spectra in Biological Technology

MarketResearch.com Announces Distribution of Spectra Intelligence Research

NEW YORK, Feb. 28 /PRNewswire/ -- As the leading distributor of market intelligence, MarketResearch.com is pleased to announce a business partnership with Spectra Intelligence which allows for the marketing and distribution of Spectra's proprietary market analyses through the MarketResearch.co...

Oclaro(TM) Hits Milestone With Clarity(TM) Fluorescence Filters

...definitive agreement with Newport Corporation which, if the transaction closes, will, among other things, provide Oclaro with a portion of the Newport spectra Physics high power laser diode business, and provide Newport with the existing New Focus business. Oclaro will continue to leverage its cutting edge, ...

Convey Computer™ Corporation Announces First Customer: University of California, San Diego at SC08 Conference

...taking up too much time and resources," said Pevzner. "Even the fastest blind search tools could run for months while analyzing millions of spectra against a large protein database. With the HC-1 we are predicting the same search could be accomplished in less than a day. This will benefit proces...

A phonon floodgate in monolayer carbon

... states resulting from electrons tunneling into graphene to be linear, following the smooth edge of the touching cones. "When we plotted the LDOS spectra of our gated graphene flakes, however, we found a gap-like feature that was centered on the Fermi energy no matter how we changed the density of char...

Unique infrared technique finds applications in nanoscience

...g a new sandwich-like optical configuration for the measurement of infrared spectra of thin films and solid-solid interfaces. The study represents the first e... This configuration allows not only the measurements of monolayer infrared spectra on a wide range of metal and nonmetal substrates with greatly improved sens...

Bruker Announces Availability of New HUPO World Congress Proteomics Posters on Web Site

...te Production by Myxococcus xanthus using ESI-TOF-MS and PCA Poster M327 Bioinformatics -- Support Vector Classification of Proteomic Profile spectra using Discrete Wavelet. Transformation and Statistical Feature Selection for Diagnosis and Biomarker Discovery Poster T174 -- Managing Proteo...

Stevens researchers provide new information about mass spectrometry

...colleagues in the Center for Mass Spectrometry at Stevens Institute of Technology. According to a study, Collision-induced dissociation (CID) mass spectra of anions derived from several hydroxyphenyl carbaldehydes and ketones were recorded and mechanistically rationalized. For example, the spectrum of m/...

Fluorescent glass SRMs are new tool for spectroscopy

...cent glass Standard Reference Materials (SRMs), about the size of a pack of a gum, whose certified values can be used to correct fluorescence emission spectra for relative intensity. SRM 2940 (Orange emission) has certified values for emission wavelengths from 500 to 800 nanometers when excited with light at...

Direct On-Membrane MS/MS Analysis using the Chemical Printer (CHIP-1000) and AXIMA-QIT

...hen conducted using the AXIMA-QIT. The obtained MS spectra are shown in Fig. 2. ...nal electrophoresis to the PVDF membrane. The MS spectra obtained from microscale on-membrane PMF analysi... The results of MS/MS ion search using these MS/MS spectra provided identification of these protein spots w...

LC-MALDI and MALDI-MS/MS for the Analysis of Complex Peptide Mixtures

...ts in higher sensitivity and means that more MS/MS spectra can be generated from the sample. Rather than acquiring all the MS/MS spectra from the same spot, with the sample being depleted with each MS/MS spectrum acquired, MS/MS spectra are generated from different positions on the MALD...

EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY

...within experimental error. A similar comparison of spectra of 1mg/mL Bovine serum albumin is shown in Figure 3. The spectra in Figure 3 have been offset by 0.1A in order to...g a spectrophotometer with a bandwidth of 1nm. The spectra of a sample containing 36g/mL DNA and 500 g/mL ser...

Protein Depletion for Plasma and Serum Proteomic Analysis

...cimens using surface enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrometry: Multi-institution correlation of spectra and identification of biomarkers. Proteomics 2005, 5 :34567-33474.
  • Barnea E, Sorkin R, Ziv T, Beer I, Admon A...

    A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

    ...ial detection can be used to decouple fluorescence spectra when multiple fluorophores are present. Although m...g are mostly broadband dyes. Thus their absorption spectra will overlap with one or more laser lines, which m... wider variety of dyes are used, separation of the spectra on the basis of emission wavelengths may not be sp...

    Complex Peptide Mixture Analysis on the Finnigan LTQ Linear Ion Trap Mass Spectrometer

    ... identification. Figures 2 and 3 compare the MS/MS spectra obtained on the two instruments. Moreover, with th...is figure also demonstrates the high quality MS/MS spectra (Figure 4C) produced from a low abundant ion in th...vity of the Finnigan LTQ, yield high quality MS/MS spectra and greater number of scans across an LC peak. Thi...

    Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

    ...horse serum containing 2 ng/mL of ACE and HEPS (40 pg on-column). The MS/MS spectra of the three analytes is also shown in Figure 1. The extracted ion chromato...ted by summing the intensities of the three most abundant ions in the MS/MS spectra as shown in Table 1. Quantitation and Line...

    Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

    ...ications in the sample have been determined, MS 2 spectra can then be exported from the Metabolite ID softwa...endent acquisition mode in which both MS and MS 2 spectra were acquired without the need to specify parent m... this mode, the acquisition software probed the MS spectra in real-time on a scan-by-scan basis to select the...

    Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

    ...e was 150C. The ion-trap collisional fragmentation spectra were obtained using collision energies of 35%. Eac...rum was followed by three Data Dependent TM MS/MS spectra of the three most intense peaks. The Dynamic Exclu...USA) which correlated the experimental tandem mass spectra against theoretical tandem mass...

    Use of On-line Size Exclusion Chromatography (SEC) for Sample Clean-up in LC/MS Analyses

    ....) Figure 3 shows chromatograms and mass spectra of the benzodiazepines in plasma analyzed on the S...on and extracted base peak chromatograms and MS/MS spectra representative of the data obtained by SEC-LC/MS/M...erum matrix. Figure 7 shows chromatograms and mass spectra of the first and last injections of the antidepres...

    Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

    ...tense product ions observed in the full-scan MS/MS spectra (Figure 2) acquired for the analyte (m/z 274 + 281...tense product ions observed in the full-scan MS/MS spectra for temazepam (m/z 255), alprazolam (m/z 274 + 281...m/z 279 + 286). Figure 4 shows the full-scan MS/MS spectra of temazepam, and alprazolam at a concentration le...

    UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)

    ...croplates are commercially available, but expensive (> $1000). There areat least three UVtransparent plastic microplates available. Figure 1 shows the spectra of the abovementioned microplates between 200 and 400 nm. Quartz is transparent throughout the wavelength range. The Corn...

    Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

    ...pplication note confirms the dynamic range and lower detection limit described by Molecular Probes in their application note (MP 07582). The OliGreen spectra is: excitation/emission: 500/520 nm as indicated in the product insert from Molecular Probes. However, they suggest using excitation/emission waveleng...

    A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

    ...oduce new types of enzymes, such as MBLs. Therefore, to enable the clinical spectra of established β -lactamase inhibitor/β -lactam combinations to b...β -lactamases. In addition, novel inhibitors with improved potency and spectra are required to combat the current plethora of enzymes (both metallo and se...

    Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

    ...ngle sample. This can be done by using a different fluorophore for each probe, assuming the probes do not interfere with each other. If their emission spectra do not overlap, detection of one in the presence of the other is straightforward. Such is the case for fluorescein (FAM)- and tetramethylrhodamine (TA...

    Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

    ...ithin cells 2 . However, there have been some technical difficulties associated with the use of GFP. One major concern is the excitation and emission spectra of the protein. Wild type GFP has two excitation peaks, a major one at 395 nm (in the long UV range) and a smaller one at 475 nm (blue) with an emissi...

    Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

    ...cent, causing a high background. If the absorption spectra of the substrate and product overlap, the substrat...are ~342 nm/441 nm. Therefore, both the excitation spectra and emission spectra overlap. Figure 1 shows the substrate and product ...

    Time gated separation of lanthanide luminescence

    ... The individual spectra of Tb-L and Eu-L complexes are shown in Figs. 3 an... Careful choice of gating parameters allows spectra from this mixture to be obtained selectively from ...l profile with time. A number of complete emission spectra were recorded while automatically incrementing the...

    Cyclodextrin enhances room temperature phosphorescence

    ... an analytical technique that offers well-resolved spectra and subpicogram detection limits. When polynuclear...epurified in an Alltech Oxy-Trap.

    All spectra were taken on a SPEX FLUOROLOG spectrophotomet...ission). The scan rate was 1 nm s 1 . All emission spectra were corrected for fluctuations in the intensity o...

    Photoluminescence Spectroscopy of Quantum Dots, monitored by the Spex FluoroMax

    ...ored at room temperature. Absorption spectra were monitored on a Shimadzu spectrophotometer ... data. Photoluminescence spectra were recorded using a Spex FluoroMax spectrofluorometer. The emission spectra were performed with an excitation wavelength ...

    Near IR capability of the FluoroLog

    ... Results Figure 1 (Nd-doped glass, below) shows clear peaks in the red and near-IR regions. Fig-ure 2 (on back) shows spectra for Er 2 O 3 powder in a Model 1933 solid-sample holder. A 780-nm cut-off filter was used in the emission spectrum. Obvious near-IR bands are visible...

    Wideband sensitivity of the FluoroLog-3

    ...se as the excitation wavelength increases. Measurement of a series of Raman spectra across the instruments usable wavelength range is much more relevant as a f... . Raman peaks are also visible at 275 nm, 531 nm, and 693 nm. The emission spectra are re-scaled to reveal the Raman bands more clearly at 667 nm and 753 nm (...

    Fluorescent pigments in living coral

    ...atures, characterizing the excitation and emission spectra of different corals in vivo and non-destructivel...r-optic probe 2 m long, the resultant fluorescence spectra were collected. For the excitation spectra, emissi...monitor of reef stability through bleaching, these spectra also serve as a catalog of coral identity to help ...

    Food science: increasing crop yields

    ....

    Results Figure 1 shows spectra taken from two different soy plants (one stressed and one unstressed) in si...t samples the unique ability to measure fluorescence spectra in the IR region of the spectrum. back to top ...

    Screening and classifying hazardous waste samples

    ... Computerized search routines for classifying spectra based on feature sets and similarity measures were... a water phase) that were not pure oils.

    All spectra presented herein were taken with the excitation ba...eference oils were characterized by their emission spectra as well as by data obtained from synchronous scann...

    Characterizing galvanizing baths

    ...on, menu-driven operation.

    Therefore, spectra can be acquired in seconds, and a time-based scan ...ere characterized by their excitation and emission spectra shown in Figures 1 through 4. The fluorescence spectra could offer a possible method for correctly mixing...

    Front-face detection for high-concentrate, opaque or solid sample

    ...tenuation of the beam. When measuring fluorescence at 90, intensity measurements may not be reproducible or detectable, and the excitation or emission spectra may appear distorted. Front-face detection offers the solution for these problems.

    Using the technique of front-face detection for opaque li...

    Immunoassay of Theophylline by fluorescence polarisation

    ...P detectors. A 450-W xenon lamp supplied the excitation.

    Results and Discussion Figure 4 shows excitation and emission spectra of bound, tagged theophylline and antibody taken with a bandpass of 7.2 nm on the excitation, and 3.6 nm on the emission spectrometers. The excitation...

    Using THERMAL DESORPTION GAS CHROMATOGRAPHY for the identification of fragrance and other components in cosmetic products

    ...ass spectral data of each of the major components and to perform a library search to identify each of these. Figures 3, 5 and 7 show some typical mass spectra taken from the liquid soap chromatogram and Figures 4, 6 and 8 show the corresponding results from the spectral library search. Figures 9 to 15 show t...

    Polymer Degradation Mechanisms Encountered in Pyrolysis-GC/MS

    ..., the pattern consists of triplets of peaks, as shown in the expanded chromatogram in Figure 2. These are all normal hydrocarbons, and as shown by the spectra in Figure 3, they are identified as the alkane, alkene and diene of increasing chain lengths. Other polyolefins, such as polypropylene, polybutylene a...

    Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System

    ...dentifications with high-sensitivity MS/MS and MS3 spectra to confirm the presence and structure of the metab...re 4 shows the enhanced resolution and product ion spectra that provided excellent confirmation and assignmen...ere ten. As with the other approaches, product ion spectra provided characterization and confirmation of the ...

    Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum

    ...de mass fingerprinting was performed on low intensity bands that did not correspond to the intact molecular weight of albumin. Figure 4 shows the mass spectra performed on peptide digests from the bands with corresponding protein identification using SwissProt. Most of these bands were identified as human se...
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