Silencing

Custom and library siRNA for efficient gene silencing

High-quality short interfering RNA (siRNA) from QIAGEN allows efficient gene silencing in eukaryotic cell culture by targeting complementary endogenous mRNA. This effect, termed RNA interference (RNAi), enables targeted gene silencing in functional genomics studies. Now, QIAGEN offers a custom siRNA oligonucleotide syn...

Custom and library siRNA for efficient gene silencing

High-quality short interfering RNA (siRNA) from QIAGEN allows efficient gene silencing in eukaryotic cell culture by targeting complementary endogenous mRNA. This effect, termed RNA interference (RNAi), enables targeted gene silencing in functional genomics studies. Now, QIAGEN offers a custom siRNA oligonucleotide syn...

RNAi Gene Silencing System

Gene silencing by RNA interference (RNAi) in eukaryotic cell cultures requires double-stranded molecules known as short interfering RNA (siRNA). QIAGEN is a licensed supplier of siRNA, offering a custom siRNA oligonucleotide synthesis service , <a href="http://www.biocom...

Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*

Center for Neurologic Diseases, Brigham and Womens Hospital, Harvard Medical School, Boston, MA, USA Genetic manipulations using neuronal cells are notoriously difficult. Therefore, efficient delivery of short interfering RNA (siRNA)...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

One of the RNA silencing products available from Ambion is a kit for the synthesis of siRNA, the <A href="http://www.ambion.com/catalog/CatNum.php?1620">Silencer <A href="http://www.ambion.com/catalog/CatNum.php?16000"> siRNA Construction Kit . While this kit is very cost effective...

RNA Interference and Gene Silencing: History and Overview

May 20, 2002 Post-transcriptional gene silencing (PTGS), which was initially considered a bizarre phenomenon limited to petunias and a few other plant species, is now one of the hottest topics in molecular biology (<A href="#1">1 ). In the last few years, it has become clear that PTGS occurs in both plants...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

Since the first description of RNA interference (RNAi) asa protection mechanism against invasion of foreign genesappeared a few years ago, RNAi has become a powerfultool for analyzing gene function in mammalian cells. RNAiis mediated by short double-stranded RNAs that a...

Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection

In addition to the specificity andefficiency of an siRNA sequencefor a target gene, which are ofhigh importance in post-transcriptionalgene silencing (PTGS), thedelivery of siRNA into cells is crucial.An efficient method to optimizetransfection and gene silenci...

Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation

Quantitative or real time RT-PCR is one of the most commonly used techniques for quantitating changes in gene expression across samples. When working with cell or tissue samples for subsequent use in RT-PCR you must extract...

GENE SILENCING WITH PCR PRODUCTS

siRNA expression vectors offer enormous opportunities to study gene silencing. Unfortunately, the preparation of siRNA vectors is labor and time intensive. Ambion's new Express siRNA Expression Cassette Kits pr...

IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

High Quality Custom Synthesis of siRNAs Cenix Pre-designed siRNAs to Human, Mouse, and Rat Genomes Superior si...

Drive Long-Term Silencing with CMV Vectors

Ambion's newest line of plasmid siRNA expression vectors, p 4.1-CMV, feature polymerase (pol) II promoters that drive transcription of both an siRNA template and an antibiotic resistance gene. The CMV promoter is used to drive siRNA expression. This...

Enhanced siRNA Delivery and Long-term Gene Silencing

5.1 Retro System enables researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. These retroviral vectors make use of H1 or U6 Polymerase III promoters...

Fast, Accurate Assessment of siRNA-induced Gene Silencing

Cells-to-Signal Kit: Small Sample Prep for qRT-PCR Real-time, quantitative RT-PCR (qRT-PCR) is the most sensitive way to detect and quantitate mRNA and is often used to validate techniques that monitor changes in gene expression, including array analyses and RNA...

Superior Gene Silencing Using Adenoviral Vectors

Mediating RNA interference (RNAi) with recombinant adenovirus expressing siRNAs has only recently been described in the literature (1). Recombinant adenoviruses have several advantages over other methods of nucleic acid delivery. Namely, these viruses can infect dividing and...

Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs

siRNA Construction Kit uses an in vitro transcription reaction and column purification to prepare transfection-ready siRNAs. With this kit, multiple siRNAs can be prepared in ~24 hours. Below is one example of how siRNAs prepared by the Silenc...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most common m...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most common m...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

Ambion has developed a system for siRNA synthesis and purification based on in vitro transcription, the siRNA Construction Kit (patent pending). This kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. Here, resear...