Sample

Sample Preparation for Microinjection

Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 m [1]. This capillary is back loaded with the sub...

GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip expression arrays. The GeneChip Sample Cleanup Module was developed as part of an on...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently count...

Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples

MIA (Melanoma Inhibitory Activity) was cloned as asecreted protein from human melanoma cell lines [1]. MIAis expressed in and secreted from malignant melanomasand chondrocytes [2, 3]. As MIA expression in chondrocytesis dependent on the differentiation status of the cel...

Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis

Whereas in the past mass spectrometers (MS) were confined to therealm of small molecules, the development of soft ionization methodssuch as matrix-assisted laser desorption/ionization (MALDI) and electrosprayionization (ESI) revolutionized the analysis of large biomolecule...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

Gene expression profiling of material isolated by microdissection hasbecome a very popular method for analyzing cellular behavior in a microscale and is used in a wide range of research and clinical applications.Laser-assisted microdissection allows isolation of...

A Better Way to Isolate RNA AND Protein From the Same Sample

Isolate nuclear and cytoplasmic RNA and protein from cultured cells Ambion's new PARIS Kit uses a fast, simple procedure based on Ambion's RNAqueous technology to isolate bo...

Isolate Total RNA and Protein From the Same Sample

Vana PARIS Kit combines the technological advances from both the <A href="http://www.ambion.com/catalog/CatNum.php?1560">mir <A href="http://www.a...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

RNAqueous-Micro Kit Ideal for Processing Laser Capture Microdissection Samples Further development of Ambion's RNAqueous-Micro Kit has resulted in a 3-6 fold increase in total RNA yields from LCM samples. In addition, Ambion has introduced a new LCM Staining Kit that has been o...

Preserve Samples for RNA Expression Microarrays RNAlater Around the World

protects cellular RNA within specimens during sample collection and during shipping. Whether you are in the lab, in the OR, or out in the field, you can use this aqueous, non-toxic Tissue Collection:RNA Stabilization Solution from ambient temperatures down to...

RNA from LCM Samples

Juanita Gonzales, Ambion, Inc. Obtaining specific populations of cells from heterogenous samples is a major challenge in studying gene expression profiles of specific cell types. Laser capture microdissection (LCM), developed in a collaborative effort between...

RNA, DNA, and Protein from a Single Sample

Use one reagent to isolate DNA-free RNA, RNA-free DNA, and/or protein Obtain higher yields than traditional guanidine thiocyanate or cesium chloride methods Compatible with many sample sources...

Robust High Throughput RNA Isolation from Blood Samples

Mammalian whole blood is a widely used tissue for molecular assays due to abundant genetic information as well as ease and simplicity of sample collection. RNA isolation from whole blood is challenging, but Ambions MagMAX-96 Blood RNA Isolat...

RNAlater Around the World: The Art of Processing Difficult Samples

Draw on the strength of RNA to protect cellular RNA within specimens during sample collection or shipping. Whether you are in the lab or out in the field, this aqueous, non-toxic Tissue Collection:RNA Stabilization Solution functions from...

Working With Laser Capture Microdissection Samples

Laser Capture Microdissection (LCM) is a method for obtaining pure populations of cells from heterogeneous samples. LCM permits the selection and capture of cells, cell aggregates, and discrete morphological structures from thin tissue sections. For LCM, frozen and OCT embedded, or formalin-fixed and paraffin emb...

Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus

Catalog #732-6370 Yield: Dependent on sample and RNA expression levels Samples may be fresh or frozen. Collect fresh virus and isolate RNA as quickly as possible; store at 18C fo...

Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

and Aiguo Zhang, 1 Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, and Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA...

Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

T Slyker, B Gillece-Castro, M Nguyen, L Sapp, MG Brubacher, and WB Strong Presented by the Authors at The Association of Biomolecular Resource Facilities (ABRF) Denver, Colorado 2-D gel electrophoresis (2DE) is a widely used, proven method for...

Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

Derek Cody and Laura G. Leff, Department of Biological Sciences, Kent StateUniversity, Kent, Ohio Assessing bacterial genetic diversity in natural environments is problematic because of difficulties in culturing native bacteria and the large number of species that may be encountered. The morpho...

Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor

M. Adams and R. Deadman GE Healthcare, Piscataway, NJ, US GE Healthcare offers a range of DNA amplification products to meet all genomic DNA and sequencing template preparation needs. A combination of these kits with the Genesis Freedom™ Robotic Sample Processor (RSP) from Tecan (Fig 1) creates a total solution for high-throu...

Fast Multi-Residue Pesticide Analysis in Soil and Vegetable Samples

The strict detection limits that are required for the quantitation of pesticide residues in food and environmental samples requires the use of very sensitive, selective, and rugged LC/MS/MS technologies. In this application note the benefits of using LC/MS/MS for this type of analysis...

Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS

A very straightforward method for the analysis of the very polar organophosphorus pesticides (OPs), acephate, methamidophos, omethoate, oxydemeton-methyl, vamidothion, and monocrotophos in water has been developed. Large volume (1 ml) water samples were directly injected onto a RP18 HPLC column with a polar endcapping. The compounds were ionized using APCI and detected on a tandem mas...

Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer

. The purpose of this work was to evaluate the abil...

Technical Specifications Accelerating Your Research with the BD FACSCanto High Throughput Sampler Option

Accelerating Your Research with the BD FACSCanto High Throughput Sampler Option The new BD High Throughput Sampler (HTS) provides fully automated and rapid sample acquisition. It is designed to speed through a microtiter plate in 15 minutes* with sample carryover to...

Maintaining High Sensitivity when Working with Small-Volume Samples

20 L, 50 L, 250 L, or 1 mL <P > <br >enzymes amino acids <P > <br>photon-counting <P >Samples can be valuable. Either you dont want to waste them, or you may not have very much with which to start. Biological prot...

Front-face detection for high-concentrate, opaque or solid sample

When working with highly-concentrated, opaque, or solid samples such as blood, paint, optical brighteners, living cells, polymer films, or phosphors, front-face fluorescence detection offers significant advantages for quantitative and qualitative fluorescence measurements. Highly concentrated and opaque liquids typically have problems with self-absorption and complete attenuation of the be...

Screening and classifying hazardous waste samples

Timely and accurate analysis of hazardous materials is fundamental to environmental protection. In addition to the obvious necessity of identifying a suspected contaminant, a detailed analytical profile can help trace the source of a dangerous material and contribute to selecting the best containment and cleanup strategy. <P>A substantia...

Use of On-line Size Exclusion Chromatography (SEC) for Sample Clean-up in LC/MS Analyses

Use of LC/MS has experienced a dramatic increase in applications throughout the drug discovery and development process, from high-throughput screening...

Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

Thermo Electron Corporation, Somerset, NJ, USA Acrylamide has been identified as a potential human carcinogen. This is important not only because acrylamide is a common industrial chemical, but acry...

Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard

Thermo Electron Corporation, San Jose, CA, USA The Deferred Standard (DS) concept was introduced more than 40 years ago with the purpose of monitoring the reliability of process chromatography. The DS consi...

TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds

Using high purity Type B silica, Tosoh has introduced two new columns with differing surface treatment procedures and carbon content levels to provide unique selectivities for a wide range of sample types. In recent years, many new octad...

Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

James F. Eliason, Ph.D., Asterand plc The path to identification of new and improved drugs for the treatment of human diseases often begins with the identification of novel gene targets through RNA expression analysis. Most high throughput gene expression profiling studies use high density microarrays, wh...