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Saccharomyces in Biological Technology

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse Paul Gavin # and Mark Prescott # , Ph.D Daren J. Fyfe, Ph.D* # Department o...

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae : Detection in vivo using the Varian Cary Eclipse Paul Gavin# and Mark Prescott # , Ph.D Daren J. Fyfe, Ph.D * # Department of Bi...

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

Contributed by Alan T. Bakalinsky Department of Food Science and Technology, Oregon State University, Corvallis, OR 97331-6602 Abstract Chromosomal profiles of wine strains of Saccharomyces cerevisiae were compared to those of a stan...

J. Craig Venter Institute Researchers Publish Significant Advance in Genome Assembly Technology

...A segments. During this building process the team found that E. coli had difficulty reproducing the large DNA segments, so they turned to the yeast saccharomyces cerevisiae. They were then able to finish creating the synthetic bacterial genome using a method called homologous recombination (a process that cell...

Brewing better beer: Scientists determine the genomic origins of lager yeasts

...identified the genomic origins of the lager yeast saccharomyces pastorianus , which could help brewers to better c...ds of years, ale-type beers have been brewed with saccharomyces cerevisiae (brewer's or baker's yeast). In contr...ar-round low-temperature fermentations possible. saccharomyces pastorianus , the yeast used in lager brewing, is ...

New yeast trick for eating favorite food: Details in Nature paper

...st discovery of post-transcriptional gene regulation in a key model for gene regulation in higher organisms: the galactose genetic system in the yeast saccharomyces cerevisiae. Molecular biologists have long thought that the primary mechanism for regulating genes is through proteins called transcription fa...

Growing old together: Yeast, worms and people may age by similar mechanisms

...y, a group of researchers led by Drs. Matt Kaeberlein and Brian Kennedy of the University of Washington conducted a genome-wide analysis of the yeast saccharomyces cerevisiae and the nematode worm Caenorhabditis elegans , to identify genes that may regulate aging in humans. Nematodes and humans are more simila...

Venter Institute Scientists Create First Synthetic Bacterial Genome

...hemical assembly, the team found they could use homologous recombination (a process that cells use to repair damage to their chromosomes) in the yeast saccharomyces cerevisiae to rapidly build the entire bacterial chromosome from large subassemblies. "This extraordinary accomplishment is a technological marvel...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

... fermentor at optimum specific-growth rate. Biotechnol. Bioeng., 33, 115-125 Aiba, S., S. Nagai and Y. Nishizaqa. 1976. Fed-batch culture of saccharomyces cerevisiae : a perspective of computer control to enhance the productivity in bakers yeast cultivation. Biotechnol Bioeng., 18, 1001-1011 ...

New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

...lular Weight (g/L) against Cell concentration (cells/mL). Figure 2: NucleoCounter YC-100 raw image of yeast cells in suspension ( saccharomyces cerevisiae ) after Reagent YC-100 treatment using 100-fold dilution. Cell count is approx. 8 x 10 7 /mL. The objects have similar fluorescence intensi...

Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ

... References 1. Ficarro, S. B. et al . Phosphoproteome analysis by mass spectrometry and its application to saccharomyces cerevisiae. Nat. Biotechnol . 20 , 301–305 (2002). 2. Application note: Highly sensitive phosphopeptide analysis using Ett...

Electroporation of Yeast Artificial Chromosomes

...to obtain reasonable efficiencies with larger YACs. Several electroporation protocols have recently been reported for the transformation of saccharomyces cerevisiae with supercoiled plasmids. 3-6 In an attempt to improve the currently used YAC cloning procedure, we investigated th...

Transformation of Filamentous Fungi by Microprojectile Bombardment

... such as obligate plant pathogens. Biolistic transformation of saccharomyces cerevisiae cells was optimized using the PDS-1000/He system (Hei..., J. C. and Johnston, S. A., Biolistic nuclear transformation of saccharomyces cerevisiae and other fungi, Curr. Genet., 17 , 97-103 (1990). ...

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

... Stable transformation of maize, Chlamydomonas reinhardtii, and saccharomyces cerevisiae nuclear genomes, as well as C. reinhardtii chloroplas... Stable transformation of yeast mitochondria. A derivative of saccharomyces cerevisiae strain MCC109 (MATα ade2-101 ura3-52 kar1-1) ...

Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

...expression of a wild-type URA 3 gene in an auxotrophic mutant of saccharomyces cerevisiae. The microparticle variables include the size, amount..., J. C. and Johnston, S. A., Biolistic nuclear transformation of saccharomyces cerevisiae and other fungi, Curr. Genet., 17 , 97-103 (1990). ...

The Bio-Rad Silver Stain, Rev E

...arly mitotic events in a cell-free system, Cell 1, 165175 (1985) 59. Miyajima A et al., Secretion of mature mouse interleukin-2 by saccharomyces cerevisiae: use of a general secretion vector containing promoter and leader sequences of the mating pheromone α-factor, Ge...

Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

...press, Rajamaki and Valkonen 1999). References Armaleo, D., et al., Biolistic nuclear transformation of saccharomyces cerevisiae and other fungi, Curr. Genet., 17 , 97103 (1990) Bartels, R., Potato virus A, CMI/AAB Desc. Pl. Viruses , 54 (1971)...

Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping

... 50 minutes over 55 hours. Under these conditions, the chromosomes of Schizosaccharomyces pombe (closed circles, lanes 4 and 6), saccharomyces cerevisiae (open squares, lanes 3 and 5), lambda phage concatomers (open circles, lane 2), and the larger fragments of Hind III-...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

... Sample material saccharomyces cerevisiae was grown in YPD medium (50 g/l, rich) and SD base me...d with and without amplification Total RNA from saccharomyces cerevisiae grown in rich or minimal medium was labeled with Cy3 and Cy5 b...

RNeasy Mini, Midi, and Maxi Kits

...ified with the RNeasy Maxi Kit. 10 g of total RNA isolated from each source was loaded per lane. All tissues were from mouse. Yeast: saccharomyces cerevisiae; E. coli: HB101. 32P-labeled probes recognized (G) GAPDH; (E) translation elongation factor EF-1a ; and (O) outer membrane ...

Saccharomyces cerevisiae

.... 4308 915.531 12/2001 Microorganism saccharomyces cerevisiae Cell type Yeast Molecules injected Plasmi...

Saccharomyces cerevisiae

.... 4308 915.531 12/2001 Microorganism saccharomyces cerevisiae Cell type Yeast Molecules injected Plasmi...

Saccharomyces cerevisiae

.... 4308 915.531 12/2001 Microorganism saccharomyces cerevisiae Cell type Yeast Molecules injected Plasmi...

Saccharomyces cerevisiae

.... 4308 915.531 12/2001 Microorganism saccharomyces cerevisiae Cell type Yeast Molecules injected Plasmi...

Yeast Protein Production System Features High Yields and One-Step Purification

...1 fragment, which provides an origin of replication for the vector to replicate autonomously in S. pombe; (3) a LEU2-d gene from saccharomyces cerevisiae, which serves as a selection marker for transforming the expression vector into S. pombe cells (strain SPQ01) and (4)...

A New C-Terminal GST Vector for Protein Production in S. pombe

... ars1 fragment, providing the origin of replication for the vector to replicate autonomously in S. pombe; (3) a LEU2-d gene from saccharomyces cerevisiae, serving as a selection marker for transforming the expression vector into S. pombe cells (strain SP-Q01, leu1-32h - ...
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